Abstract: A method and apparatus that imprints protein binding membranes with predetermined patterns, determined by (a) a pattern cut in a porous foam, or (b) a circular pattern achieved by contacting a drop of protein containing solution. The apparatus and process are particularly well adapted to forming the active surface for devices that detect antigens with antibodies.

Abstract: The performance of a waste-water reclamation system is monitored by introducing a non-pathogenic marker virus, bacteriophage F.sub.2, into the waste-water prior to treatment and, thereafter, testing the reclaimed water for the presence of the marker virus. A test sample is first concentrated by absorbing any marker virus onto a cellulose acetate filter in the presence of a trivalent cation at low pH and then flushing the filter with a limited quantity of a glycine buffer solution to desorb any marker virus present on the filter. Photo-optical detection of indirect passive immune agglutination by polystyrene beads indicates the performance of the water reclamation system in removing the marker virus. A closed system provides for concentrating any marker virus, initiating and monitoring the passive immune agglutination reaction, and then flushing the system to prepare for another sample. Peristaltic pumps are provided for volumetric control and for positive fluid displacement.