Abstract: A bioreactor with an upward flowing impeller system for controlling a mammalian cell culture process is provided. The disclosed system enables control of the cell culture process by controlling the level of dissolved carbon dioxide in the cell culture media and prevent increases in the osmolality level is achieved by enhanced stripping of carbon dioxide via surface gas exchange with little or no damage to the mammalian cells. The use of an upward flow impeller combined with vertical baffles converts the swirling motions of the cell culture media into a largely vertical flow and promotes the removal of dissolved carbon dioxide via surface gas exchange with a sweep gas flowing over the top surface of the cell culture media within the bioreactor vessel.

Abstract: A bioreactor with an upward flowing impeller system for controlling a mammalian cell culture process is provided. The disclosed system enables control of the cell culture process by controlling the level of dissolved carbon dioxide in the cell culture media and prevent increases in the osmolality level is achieved by enhanced stripping of carbon dioxide via surface gas exchange with little or no damage to the mammalian cells. The use of an upward flow impeller combined with vertical baffles converts the swirling motions of the cell culture media into a largely vertical flow and promotes the removal of dissolved carbon dioxide via surface gas exchange with a sweep gas flowing over the top surface of the cell culture media within the bioreactor vessel.

Abstract: A system and method for controlling a mammalian cell culture process are provided. Such control of the cell culture process involves control of the level of dissolved carbon dioxide in the cell culture media and the resulting ability to prevent increases in the osmolality level is achieved by enhanced stripping of carbon dioxide with little or no damage to the mammalian cells. The disclosed methods and systems of dissolved carbon dioxide stripping include enhanced surface gas exchange mechanisms within the bioreactor vessel through the use of an upward flow impeller combined with vertical baffles to convert swirling motions of the liquid into a largely vertical flow.

Abstract: Methods for controlling the level of dissolved carbon dioxide and limiting osmolality in a mammalian cell culture process to enhance cell growth, viability and density, and increase biologic product concentration and yield are provided. Such control of the level of dissolved carbon dioxide and pH as well as the resulting ability to limit osmolality in a mammalian cell culture process is achieved by adopting alternative pH control strategies and CO2 stripping techniques during a mammalian cell culture process. Such pH control techniques and carbon dioxide stripping occur without foam and with little or no damage to the mammalian cells.

Abstract: Methods for controlling the level of dissolved carbon dioxide and limiting osmolality in a mammalian cell culture process to enhance cell growth, viability and density, and increase biologic product concentration and yield are provided. Such control of the level of dissolved carbon dioxide and pH as well as the resulting ability to limit osmolality in a mammalian cell culture process is achieved by adopting alternative pH control strategies and CO2 stripping techniques during a mammalian cell culture process. Such pH control techniques and carbon dioxide stripping occur without foam and with little or no damage to the mammalian cells.

Abstract: Methods for controlling the level of dissolved carbon dioxide and limiting osmolality in a mammalian cell culture process to enhance cell growth, viability and density, and increase biologic product concentration and yield are provided. Such control of the level of dissolved carbon dioxide and pH as well as the resulting ability to limit osmolality in a mammalian cell culture process is achieved by adopting alternative pH control strategies and CO2 stripping techniques during a mammalian cell culture process. Such pH control techniques and carbon dioxide stripping occur with little or no damage to the mammalian cells.

Abstract: A system and method for controlling a mammalian cell culture process are provided. Such control of the cell culture process involves control of the level of dissolved carbon dioxide in the cell culture media and the resulting ability to prevent increases in the osmolality level is achieved by enhanced stripping of carbon dioxide with little or no damage to the mammalian cells. The disclosed methods and systems of dissolved carbon dioxide stripping include enhanced surface gas exchange mechanisms within the bioreactor vessel through the use of an upward flow impeller combined with vertical baffles to convert swirling motions of the liquid into a largely vertical flow.

Abstract: A system and method for sterilizing a cryogen is provided. In the disclosed embodiment a liquid cryogen is first vaporized and subsequently compressed prior to undergoing sterilization using biological filters. The compressed, sterilized cryogen vapor is then pre-cooled, preferably with the cryogen being vaporized, to produce a partially condensed, cool, sterile cryogen stream and then further condensed using the liquid cryogen to produce a sterilized liquid cryogen.

Abstract: A system for the recovery of volatile compounds comprising a direct contact condenser and an indirect heat exchanger, wherein the heat exchanger is driven by cryogenic fluid, and a heat exchange fluid, having the same composition as the volatile compound, recirculates between the condenser and the heat exchanger.

Abstract: A vent gas stream containing volatile organic compounds is passed continuously through a condenser in counter-current flow with respect to cold clean vent gas combined with a cryogenic refrigerant. The volatile organic compounds are condensed at various temperatures inside the condenser, and are separately recovered at very low levels of refrigerant utilization.

Abstract: A gas injection and heating device and method for the bubbling of a gas into a body of hot liquid to be interacted with the gas. The device comprises an elongate heat exchange gas container, designed to be immersed in the hot liquid to pre-heat the gas in situ by heat exchange with the liquid. Cold gas is supplied to the elongate gas container, circulated therethrough to become heated to the liquid temperature, and then released from a nozzle into the depth of the liquid in the form of small bubbles of hot gas having a large liquid interfacial mass transfer area.

Abstract: The invention relates to a process for recovering heat from deodorized edible oils and stabilizing the same comprising: introducing or injecting non-condensible inert gas into hot deodorized edible oil as the hot deodorized edible oil is cooled by indirectly heat exchanging with crude oil to be deodorized.

Abstract: The invention relates to a process for deodorizing edible oils and/or fats comprising: heating edible oil and/or fat to an elevated temperature; introducing or injecting non-condensible inert gas into said edible oil and/or fat to strip or remove substances that impart disagreeable odor and taste to said edible oil and/or fat; and recovering the resulting deodorized edible oil and/or fat, wherein an amount of said non-condensible inert gas introduced or injected is substantially less than the theoretically required amount for deodorizing said edible oil and/or fat. The condensible gas may be preheated before its introduction into the edible oil and/or fat.

Abstract: The invention relates to a process for recovering heat from deodorized edible oils and stabilizing the same comprising: introducing or injecting non-condensible inert gas into hot deodorized edible oil as the hot deodorized edible oil is cooled by indirectly heat exchanging with crude oil to be deodorized.

Abstract: The invention relates to a process for deodorizing edible oils and/or fats comprising: heating edible oil and/or fat to an elevated temperature; introducing or injecting non-condensible inert gas into said edible oil and/or fat to strip or remove substances that impart disagreeable odor and taste to said edible oil and/or fat; and recovering the resulting deodorized edible oil and/or fat, wherein an amount of said non-condensible inert gas introduced or injected is substantially less than the theoretically required amount for deodorizing said edible oil and/or fat. The condensible gas may be preheated before its introduction into the edible oil and/or fat.