Abstract: Provided herein are IL-2 muteins, IL-2 mutein Fc-fusion molecules, anti-IL-2 antibodies, and complexes comprising an anti IL-2 antibody bound to an IL-2 cytokine that preferentially expand and activate T regulatory cells and are amenable to large scale production. Also provided herein are variant human IgG1 Fc molecules lacking or with highly reduced effector function and high stability despite lacking glycosylation at N297. Also provided herein are linker peptides that are glycosylated when expressed in mammalian cells. Also provided herein are methods of making and using the compositions of the present invention.

Abstract: Patients diagnosed with a cancer harboring an IDH-1 mutation can be treated by the administration of a therapeutically effective amount of a pharmaceutical composition comprising Compound 1, a selective inhibitor of 2-HG production from mIDH-1 enzymes including the R132 mutations R132C, R132H, R132L, R132G, and R132S.

Abstract: A vacuum therapy device for treatment of a defect internal of a human or animal body, the device comprising: a suction tube extending from: (i) a proximal portion for coupling to a source of negative pressure outside the body, to (ii) a distal portion for providing suction proximal to the defect when inserted into the body; a porous medium coupled to the distal portion of the suction tube to provide suction to the defect when inserted into the body for treatment thereof; and a constraining sleeve for selective compression of the porous medium, the constraining sleeve having a proximal end and a distal end, wherein the constraining sleeve is movable relative to the porous medium between: an insertion position in which the proximal and distal ends of the sleeve are arranged at the distal portion of the suction tube so that the sleeve at least partially surrounds the porous medium to retain the porous medium in a compressed state during insertion into the body; and a treatment position in which the porous medium

Abstract: An apparatus for fitting to a wellbore, the apparatus including: a self-propelled downhole tool for deployment within the wellbore, and configured to propel itself along at least part of a length of the wellbore; and a lubricator for fitting to a wellhead of the wellbore via a valve system providing communication between the lubricator and the wellbore, and for housing the self-propelled downhole tool when in a stowed position, wherein the lubricator includes an input port for receiving data from a remote unit, the input port being in electrical communication with the self-propelled downhole tool when in the stowed position, and wherein the received data includes instructions for operating the self-propelled downhole tool, and associated self-propelled downhole tool, lubricator and method.

Abstract: A separator for separating constituents of well fluid produced by a well is provided. The separator includes a vessel for receiving well fluid such that a first fluid constituent overlies a second fluid constituent within the vessel, a collection pipe having an opening for collecting a first fluid constituent from the vessel, and a positioner connected to the collection pipe for rotating the collection pipe about an axis to adjust a height of the opening within well fluid in the vessel. A method and system are also provided.

Abstract: A spark plug, including a metallic shell, an insulator, a center electrode, and a ground electrode. The insulator includes a first outer surface at least partly along an exposed portion and a second outer surface at least partly along an unexposed portion. The first outer surface extends between the exposed-unexposed interface to a tip merge portion adjacent a distal end of the insulator. The first outer surface and the second outer surface meet at a nose surface transition. At the nose surface transition, the first outer surface is disposed at a first angle with respect to a central axis of the spark plug, and the second outer surface is disposed at a second angle with respect to the central axis of the spark plug. The first angle is larger than the second angle, and the nose surface transition is located within the unexposed portion or at the exposed-unexposed interface.

Abstract: A spark plug, including a metallic shell, an insulator, a center electrode, and a ground electrode. The insulator includes a first outer surface at least partly along an exposed portion and a second outer surface at least partly along an unexposed portion. The first outer surface extends between the exposed-unexposed interface to a tip merge portion adjacent a distal end of the insulator. The first outer surface and the second outer surface meet at a nose surface transition. At the nose surface transition, the first outer surface is disposed at a first angle with respect to a central axis of the spark plug, and the second outer surface is disposed at a second angle with respect to the central axis of the spark plug. The first angle is larger than the second angle, and the nose surface transition is located within the unexposed portion or at the exposed-unexposed interface.

Abstract: An assay method and device can perform at least one (e.g., at least two) assays on a single aliquot of a sample liquid. The device can mix a sample liquid with assay reagents including magnetically susceptible particles. The device is configured to create a sample liquid-air interface with the sample liquid. The magnetically susceptible particles can be located (via an applied magnetic field) at the liquid-air interface when a second liquid contacts the interface to form a liquid-liquid interface. The magnetic particles travel across the liquid:liquid interface to the second liquid. The magnetically susceptible particles are configured to transport an analyte across the interface into the second liquid. An assay for the analyte is performed in the second liquid. An assay for another analyte can also be performed in the sample liquid.

Abstract: The present invention relates to stilbene and quinine compounds related to combretastatin A-4 and their use as anticancer compounds and prodrugs. The compounds include those with an alkyl group on the double bond of cis- or trans-stilbenes, compounds with one or more (and preferably 2 or 3) alkyl group substituents on the stilbene A ring, compounds with an alkoxy group other than methoxy at position 3, 4, and/or 5 of the stilbene A ring, compounds (or prodrugs) in which BBOC amino acid esters are formed with the phenolic hydroxyl at the 3-position of the B ring and compounds (or prodrugs) based on a benzoquinone B ring. The present invention further relates to the photochemical reactions of stilbene compounds, either the above compounds disclosed for the first time herein or compounds based on prior stilbenes.

Abstract: The present invention relates to compounds of formula (I): wherein A=formula: (a) or formula: (b); X is oxygen or methylene; Y is C3-6 alkyl or aryl; Z is oxygen or C1-3 alkyl; R1 is hydrogen or C1-3 alkyl; R2 is hydrogen or C1-3 alkyl; the or each R3 is separately C1-3 alkyl or halo; the or each R4 is separately C1-3 alkyl or halo; p is 0 to 4; q is 0 to 4; m is 0 or 1; and n is 1 to 3. These compounds are useful as kinase inhibitors for the treatment of cancer and other diseases.

Abstract: The present invention relates to stilbene and quinine compounds related to combretastatin A-4 and their use as anticancer compounds and prodrugs. The compounds include those with an alkyl group on the double bond of cis- or trans-stilbenes, compounds with one or more (and preferably 2 or 3) alkyl group substituents on the stilbene A ring, compounds with an alkoxy group other than methoxy at position 3, 4, and/or 5 of the stilbene A ring, compounds (or prodrugs) in which BBOC amino acid esters are formed with the phenolic hydroxyl at the 3 -position of the B ring and compounds (or prodrugs) based on a benzoquinone B ring. The present invention further relates to the photochemical reactions of stilbene compounds, either the above compounds disclosed for the first time herein or compounds based on prior stilbenes.

Abstract: A method of determining the presence or amount of analyte in a fluid sample, which comprises: contacting a fluid sample with a binding reagent that comprises a plurality of cleavable species and wherein said species, when cleaved, are detectable using electrochemical means; separating any binding reagent-analyte complex that forms from the unbound binding reagent; cleaving the cleavable species from the immobilized binding reagent-analyte complex; and detecting the cleaved species using electrochemical means.

Abstract: The present invention relates to stilbene and quinone compounds related to combretastatin A-4 and their use as anticancer compounds and prodrugs. The compounds include those with an alkyl group on the double bond of cis or trans-stilbenes, compounds with one or more (and preferably 2 or 3) alkyl group substituents on the stilbene A ring, compounds with an alkoxy group other than methoxy at position 3, 4, and/or 5 of the stilbene A ring, compounds (or prodrugs) in which BOC amino acid esters are formed with the phenolic hydroxyl at the 3-position of the B ring and compounds (or prodrugs) based on a benzoquinone B ring. The present invention further relates to the photochemical reactions of stilbene compounds, either the above compounds disclosed for the first time herein or compounds based on prior art stilbenes.

Abstract: The disclosed system, apparatuses, methods, and media prompt a person through a process of identifying and filling out a form(s) required to complete a task. An automated interrogation stage questions the user to determine a change of status that the user desires to effect with the organization. The interrogation stage can be dynamic and interactive, so that questions and forms provided to the user are dependent upon answers previously submitted by the user. The user is thus guided through the process of identifying the tasks and forms required of the user to affect a change of status with the organization.

Abstract: This invention relates to methods and apparatus for detecting the presence of analyte in a sample, in particular to luminescence-based methods for such detection.

Abstract: The present invention relates to stilbene and quinone compounds related to combretastatin A-4 and their use as anticancer compounds and prodrugs. The compounds include those with an alkyl group on the double bond of cis or trans-stilbenes, compounds with one or more (and preferably 2 or 3) alkyl group substituents on the stilbene A ring, compounds with an alkoxy group other than methoxy at position 3, 4, and/or 5 of the stilbene A ring, compounds (or prodrugs) in which BOC amino acid esters are formed with the phenolic hydroxyl at the 3-position of the B ring and compounds (or prodrugs) based on a benzoquinone B ring. The present invention further relates to the photochemical reactions of stilbene compounds, either the above compounds disclosed for the first time herein or compounds based on prior art stilbenes.

Abstract: The present invention relates materials and methods for synthesizing stilbenes, and in particular to processes for the synthesis of substituted stilbenes such as combretastatin A4. The present invention relates in particular to methods which are stereoselective for either the cis or the trans isomer of the substituted stilbene, using a Perkin-type condensation of an arylacetic acid and a substituted benzaldehyde, followed by a decarboxylation reaction to produce the substituted cis-stilbenes or a Suzuki-type reaction involving a Z or E-ethenyl halide and a substituted boronic acid in the presence of a palladium catalyst to produce specifically either the Z or E-isomer of substituted stilbenes.

Abstract: An improved software protection mechanism is disclosed whereby both the “asset files” of a software program and the core executable program itself are transformed in such a way that any redistribution of the modified executable program would also require redistribution of the corresponding transformed asset files. In operation, a file transformation module is used to intercept any file activity from the operating system. The file transformation module will perform the required reverse transformation only if the invoking request to the operating system is identified as being for transformed data bound to or from the modified executable program. A protection module containing asset lists, calling process identification information, transformation keys, and optionally other information such as transformation algorithms is used in association with the file transformation module.