Abstract: A method for nucleic acid sequencing includes receiving a plurality of observed or measured signals indicative of a parameter observed or measured for a plurality of defined spaces; determining, for at least some of the defined spaces, whether the defined space comprises one or more sample nucleic acids; processing, for at least some of the defined spaces, the observed or measured signal to improve a quality of the observed or measured signal; generating, for at least some of the defined spaces, a set of candidate sequences of bases for the defined space using one or more metrics adapted to associate a score or penalty to the candidate sequences of bases; and selecting the candidate sequence leading to a highest score or a lowest penalty as corresponding to the correct sequence for the one or more sample nucleic acids in the defined space.

Abstract: Provided are methods and compositions for obtaining functionally enhanced derivative effector cells obtained from directed differentiation of genomically engineered iPSCs. The derivative cells provided herein have stable and functional genome editing that delivers improved or enhanced therapeutic effects. Also provided are therapeutic compositions and uses thereof comprising the functionally enhanced derivative effector cells alone, or with antibodies or checkpoint inhibitors in combination therapies.

Abstract: An axle assembly for a working vehicle is provided. The axle assembly comprises an axle comprising a casing and a driveshaft located within the casing. The axle assembly also comprises a wheel hub for mounting a wheel thereto, wherein the wheel hub is located at an outboard end of the axle and comprises a wheel driving element which is coupled to the driveshaft by a gear arrangement. The axle assembly also comprises a brake system comprising a service brake configured to apply a braking force to the driveshaft, and an auxiliary brake configured to apply a braking force to the wheel driving element.

Abstract: A transmission system for a working vehicle includes a hydraulic drive motor 38 and a transmission 100. The transmission includes an output member 102; a first input member 104 connectable to a prime mover, wherein the transmission is operable to transmit drive from the first input member via a first drive path 108 to the output member in a mechanical drive mode; and a second input member 106 drivable by the operation of the hydraulic drive motor, wherein the transmission is operable to transmit drive from the second input member via a second drive path 110 to the output member in a hydrostatic drive mode. The transmission is configured so that there is a fixed ratio of rotation speed of the first input member to rotation speed of the output member in the mechanical drive mode.

Abstract: A leverage device for use with a hand truck, the leverage device includes a body extending from a top end to a bottom end and from a first side end to a second side end, the body having a front surface and a back surface; and an opening extending from the first side end to the second side end; the opening receives an axle of the hand truck; and the front surface receives a user's foot to apply leverage to the hand truck.

Abstract: A method for nucleic acid sequencing includes receiving a plurality of signals indicative of a parameter measured for a plurality of defined spaces, at least some of the defined spaces including one or more sample nucleic acids, the signals being responsive to a plurality of nucleotide flows introducing nucleotides to the defined spaces; determining, for at least some of the defined spaces, whether the defined space includes a sample nucleic acid; processing, for at least some of the defined spaces determined to include a sample nucleic acid, the received signals to improve a quality of the received signals; and predicting a plurality of nucleotide sequences corresponding to respective sample nucleic acids for the defined spaces based on the processed signals and the nucleotide flows.

Abstract: Provided are methods and compositions for obtaining functionally enhanced derivative effector cells obtained from directed differentiation of genomically engineered iPSCs. Embodiments of derivative cells provided herein have stable and functional genome editing that delivers improved or enhanced therapeutic effects. Also provided are therapeutic compositions and the use thereof comprising functionally enhanced derivative effector cells alone, or with antibodies or checkpoint inhibitors in combination therapies.

Abstract: A method for nucleic acid sequencing includes receiving a plurality of signals indicative of a parameter measured for a plurality of defined spaces, at least some of the defined spaces including one or more sample nucleic acids, the signals being responsive to a plurality of nucleotide flows introducing nucleotides to the defined spaces; determining, for at least some of the defined spaces, whether the defined space includes a sample nucleic acid; processing, for at least some of the defined spaces determined to include a sample nucleic acid, the received signals to improve a quality of the received signals; and predicting a plurality of nucleotide sequences corresponding to respective sample nucleic acids for the defined spaces based on the processed signals and the nucleotide flows.

Abstract: There is provided a control system for controlling two or more illuminates of a multi-modality ophthalmic imaging system that are arranged to generate light for imaging an eye, the control system comprising: a processor which selects, from the illuminates and based on a selected imaging modality of the ophthalmic imaging system, one or more illuminates that are to be used to image the eye, and to generate an input signal identifying the selected one or more illuminates. The control system further comprises a logic block, which receives the input signal and comprises logic that determines a respective operating power for each illuminate of the selected one or more illuminates, based on the received input signal. The logic block also generates a respective control signal for each illuminate of the selected one or more illuminates, each control signal indicating the respective operating power determined for the respective illuminate.

Abstract: An improved air compressor is provided. The air compressor includes a cylinder block having a head deck that defines a piston bore for reciprocal movement of a piston therein. The head deck further includes first and second ramped surfaces that slope downward toward the peripheral edge of the piston bore. A valve plate is in direct contact with the upper surface of the head deck, the valve plate having a leaf valve. During an intake stroke of the piston, the leaf valve deflects downward into direct engagement with the first and second ramped surfaces. Because the air compressor lacks a lower gasket between the valve plate and the head deck, the cost and assembly time associated with the lower gasket are eliminated.

Abstract: A method for nucleic acid sequencing includes receiving a plurality of observed or measured signals indicative of a parameter observed or measured for a plurality of defined spaces; determining, for at least some of the defined spaces, whether the defined space comprises one or more sample nucleic acids; processing, for at least some of the defined spaces, the observed or measured signal to improve a quality of the observed or measured signal; generating, for at least some of the defined spaces, a set of candidate sequences of bases for the defined space using one or more metrics adapted to associate a score or penalty to the candidate sequences of bases; and selecting the candidate sequence leading to a highest score or a lowest penalty as corresponding to the correct sequence for the one or more sample nucleic acids in the defined space.

Abstract: An improved air compressor is provided. The air compressor includes a cylinder block having a head deck that defines a piston bore for reciprocal movement of a piston therein. The head deck further includes first and second ramped surfaces that slope downward toward the peripheral edge of the piston bore. A valve plate is in direct contact with the upper surface of the head deck, the valve plate having a leaf valve. During an intake stroke of the piston, the leaf valve deflects downward into direct engagement with the first and second ramped surfaces. Because the air compressor lacks a lower gasket between the valve plate and the head deck, the cost and assembly time associated with the lower gasket are eliminated.

Abstract: A method for nucleic acid sequencing includes receiving a plurality of observed or measured signals indicative of a parameter observed or measured for a plurality of defined spaces; determining, for at least some of the defined spaces, whether the defined space comprises one or more sample nucleic acids; processing, for at least some of the defined spaces, the observed or measured signal to improve a quality of the observed or measured signal; generating, for at least some of the defined spaces, a set of candidate sequences of bases for the defined space using one or more metrics adapted to associate a score or penalty to the candidate sequences of bases; and selecting the candidate sequence leading to a highest score or a lowest penalty as corresponding to the correct sequence for the one or more sample nucleic acids in the defined space.

Abstract: A method for nucleic acid sequencing includes receiving a plurality of signals indicative of a parameter measured for a plurality of defined spaces, at least some of the defined spaces including one or more sample nucleic acids, the signals being responsive to a plurality of nucleotide flows introducing nucleotides to the defined spaces; determining, for at least some of the defined spaces, whether the defined space includes a sample nucleic acid; processing, for at least some of the defined spaces determined to include a sample nucleic acid, the received signals to improve a quality of the received signals; and predicting a plurality of nucleotide sequences corresponding to respective sample nucleic acids for the defined spaces based on the processed signals and the nucleotide flows.

Abstract: Systems and methods providing for zone-based evacuation management. The systems and methods involve allowing a user to select zones from a user interface. The zones properties may be modified by the user. The user may create evacuation pre-plans for selected zones for different types of incidents. Different scenarios may be used in the creation of the plan. The scenarios may use simulation models to determine the affected zones and the rate of spread of the incident. The systems and methods may also generate and send notifications and alerts to the people residing within the affected zones as well as police departments and fire departments with jurisdiction over the affected areas.

Abstract: A method for nucleic acid sequencing includes receiving a plurality of signals indicative of a parameter measured for a plurality of defined spaces, at least some of the defined spaces including one or more sample nucleic acids, the signals being responsive to a plurality of nucleotide flows introducing nucleotides to the defined spaces; determining, for at least some of the defined spaces, whether the defined space includes a sample nucleic acid; processing, for at least some of the defined spaces determined to include a sample nucleic acid, the received signals to improve a quality of the received signals; and predicting a plurality of nucleotide sequences corresponding to respective sample nucleic acids for the defined spaces based on the processed signals and the nucleotide flows.

Abstract: Methods and software products for analysis of alternative splicing are disclosed. In general the methods involve normalizing probe set or exon intensity to an expression level measurement of the gene. The methods may be used to identify tissue-specific alternative splicing events.

Abstract: A method for nucleic acid sequencing includes receiving a plurality of signals indicative of a parameter measured for a plurality of defined spaces, at least some of the defined spaces including one or more sample nucleic acids, the signals being responsive to a plurality of nucleotide flows introducing nucleotides to the defined spaces; determining, for at least some of the defined spaces, whether the defined space includes a sample nucleic acid; processing, for at least some of the defined spaces determined to include a sample nucleic acid, the received signals to improve a quality of the received signals; and predicting a plurality of nucleotide sequences corresponding to respective sample nucleic acids for the defined spaces based on the processed signals and the nucleotide flows.

Abstract: A method for nucleic acid sequencing includes receiving a plurality of observed or measured signals indicative of a parameter observed or measured for a plurality of defined spaces; determining, for at least some of the defined spaces, whether the defined space comprises one or more sample nucleic acids; processing, for at least some of the defined spaces, the observed or measured signal to improve a quality of the observed or measured signal; generating, for at least some of the defined spaces, a set of candidate sequences of bases for the defined space using one or more metrics adapted to associate a score or penalty to the candidate sequences of bases; and selecting the candidate sequence leading to a highest score or a lowest penalty as corresponding to the correct sequence for the one or more sample nucleic acids in the defined space.

Abstract: Methods and software products for analysis of alternative splicing are disclosed. In general the methods involve normalizing probe set or exon intensity to an expression level measurement of the gene. The methods may be used to identify tissue-specific alternative splicing events.