Abstract: When reading and writing DRAM (dynamic random-access memory), the latency and bandwidth is often unpredictable with large variations. One reason is because all the DRAM memory banks require periodic refreshes and maintenance cycles that interrupt these accesses. DRAM refresh and maintenance cycles are synchronized with the read/write accesses in a mutually exclusive manner, hence, preventing the accesses from being interfered with by a refresh or maintenance cycle resulting in predictable latency and bandwidth performance during read/write operations.

Abstract: Methods and other embodiments are described for enabling clock waveform synthesis for, in some embodiments, tensor or graphical processors that enable shorter runtime latency, higher computational job throughput, more efficient power management, and a lower implementation cost than alternative clock waveform methods. This Abstract and the independent Claims are concise signifiers of embodiments of the claimed inventions. The Abstract does not limit the scope of the claimed inventions.

Abstract: When reading and writing DRAM (dynamic random-access memory), the latency and bandwidth is often unpredictable with large variations. One reason is because all the DRAM memory banks require periodic refreshes and maintenance cycles that interrupt these accesses. DRAM refresh and maintenance cycles are synchronized with the read/write accesses in a mutually exclusive manner, hence, preventing the accesses from being interfered with by a refresh or maintenance cycle resulting in predictable latency and bandwidth performance during read/write operations.

Abstract: Provided herein, in some aspects, is a multiplex RNA targeting system that enables live cell imaging and/or modification of multiple RNA targets. Specifically, the disclosure provides a method of live cell imaging of ribonucleic acid (RNA), or targeting RNA in a live cell, comprising: (a) delivering to a cell an RNA-editing complex that comprises a catalytically inactive Cas13 (dCas13) nuclease, a Cas 13 guide RNA (gRNA) comprising an RNA aptamer sequence, and a detectable molecule linked to an RNA-binding domain (RBD), or an RNA effector molecule linked to an RBD sequence that specifically binds to the RNA aptamer sequence; and (b) imaging the detectable molecule or RNA aptamer and RBD binding.

Abstract: Provided herein are, inter alia, compositions and methods for demethylating and methylating a target DNA sequences in a mammalian cell. The compositions and methods are, inter alia, useful for modulating the expression of a target gene, or to create a gene regulatory network.

Abstract: A network interface controller (NIC) capable of efficient management of non-idempotent operations is provided. The NIC can be equipped with a network interface, storage management logic block, and an operation management logic block. During operation, the network interface can receive a request for an operation from a remote device. The storage management logic block can store, in a local data structure, outcome of operations executed by the NIC. The operation management logic block can determine whether the NIC has previously executed the operation. If the NIC has previously executed the operation, the operation management logic block can obtain an outcome of the operation from the data structure and generate a response comprising the obtained outcome for responding to the request.

Abstract: Provided herein are, inter alia, compositions and methods for demethylating and methylating a target DNA sequences in a mammalian cell. The compositions and methods are, useful for activity modulation of a targeted gene, or to create a gene regulatory network.

Abstract: The invention described herein provides compositions and reagents for integrating large sequences (e.g., 150 bp or more) into a selected target DNA sequence via prime editing.

Abstract: The present disclosure provides methods of inducing EBV early lytic cycle genes with high specificity. These methods slow or stop cancer cell growth in vitro and in vivo.

Abstract: The invention described herein provides compositions and reagents for assembling a tripartite complex at a specific location of a target DNA. The invention also provides methods for using the complex to, for example, label a specific genomic locus, to regulate the expression of a target gene, or to create a gene regulatory network.

Abstract: Provided herein are methods of imaging non-repetitive genomic loci using unique guide ribonucleic acids (gRNAs), an RNA-guided nuclease, and a detectable conjugate.

Abstract: The invention described herein provides compositions and reagents for assembling a tripartite complex at a specific location of a target DNA. The invention also provides methods for using the complex to, for example, label a specific genomic locus, to regulate the expression of a target gene, or to create a gene regulatory network.

Abstract: A network interface controller (NIC) capable of efficient management of non-idempotent operations is provided. The NIC can be equipped with a network interface, storage management logic block, and an operation management logic block. During operation, the network interface can receive a request for an operation from a remote device. The storage management logic block can store, in a local data structure, outcome of operations executed by the NIC. The operation management logic block can determine whether the NIC has previously executed the operation. If the NIC has previously executed the operation, the operation management logic block can obtain an outcome of the operation from the data structure and generate a response comprising the obtained outcome for responding to the request.

Abstract: Provided herein, in some aspects, are compositions and methods for artificially modulating alternative splicing, for example, inducing exon inclusion and/or exon exclusion events. In some embodiments, a catalytically inactive programmable nuclease, such as dCasRx, is fused to an RNA-binding protein (or fragment or isoform thereof) and, when guided to a target of interest by a specific guide RNA (gRNA), can regulate alternative splicing in eukaryotic cells.

Abstract: There is provided a system and method for the synchronizing on-air commercial programming with interactive applications, providing interactive content synchronized with on-air television programming. There is provided a system comprising a client device with a display device and an input device and running a synchronization application. The client device receives digital data transmitted from a server, and transmits user feedback information generated from user interactions with the digital data back to the server. The digital data are interactive advertisements, such as online social games, that increase users' awareness of the advertised products or messages. The digital data are delivered to the user's client device in synchronization with the broadcasting of on-air television commercials.

Abstract: The present disclosure, in some embodiments, provides sequence detection systems (sequence detectors) for the detection of specific nucleotides sequences present in the genome of live cells (e.g., single live cells) to achieve, for example, in vivo and in situ imaging, cell selection, and/or cell ablation.

Abstract: The present disclosure provides a split intein selectable marker system for the production and selection of transgenic cells.

Abstract: Provided herein are, inter alia, compositions and methods for demethylating and methylating a target DNA sequences in a mammalian cell. The compositions and methods are, useful for activity modulation of a targeted gene, or to create a gene regulatory network.

Abstract: A multiprocessor computer system comprises a dragonfly processor interconnect network that comprises a plurality of processor nodes and a plurality of routers. The routers are operable to route data by selecting from among a plurality of network paths from a target node to a destination node in the dragonfly network based on one or more routing tables.

Abstract: Provided herein are, inter alia, compositions and methods for the delivery of enhanced demethylation activity to target DNA sequences in a mammalian cell. The compositions and methods are, useful for activity modulation of a targeted gene, or to create a gene regulatory network.