Patents by Inventor Albert Roder
Albert Roder has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 5094943Abstract: The present invention provides a process for the improved colorimetric determination of hydrogen peroxide as formed by a hydrogen peroxide-producing oxidase, by addition of a chromogenic system and measurement of the colored material formed, wherein superoxide dismutase (E.C. 1.15.1.1) is added to the reagent solution.The present invention also provides a reagent for the improved colorimetric determination of hydrogen peroxide, comprising a hydrogen peroxide-producing oxidase, a chromogenic system, a buffer and optionally adjuvant enzymes, wherein it also contains superoxide dismutase.Type: GrantFiled: January 19, 1988Date of Patent: March 10, 1992Assignee: Boehringer Mannheim GmbHInventors: Joachim Siedel, Albert Roder, Joachim Ziegenhorn
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Patent number: 4914040Abstract: The invention teaches a method for improving the ability to determine a polyvalent substance via use of an immunoaggregate during the assay. The immunoaggregate eliminates substances which can lead to incorrect results otherwise.Type: GrantFiled: March 3, 1988Date of Patent: April 3, 1990Assignee: Boehringer Mannheim GmbHInventors: Helmut Lenz, Ellen Mossner, Werner Stock, Albert Roder, Harald Haug, Robert C. McCarthy
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Patent number: 4816393Abstract: The present invention provides 1-methylhydantoinase, which hydrolyses 1-methylhydantoin in the presence of a nucleoside triphosphate and of polyvalent metal ions.The present invention also provides a process for obtaining 1-methylhydantoinase and a reagent containing it.Furthermore, the present invention provides a process for the determination of creatinine by the conversion of the creatinine with creatinine deiminase (E.C. 3.5.4.21) into 1-methylhydantoin, hydrolysis of the latter with the 1-methylhydantoinase in the presence of nucleoside triphosphate and of polyvalent metal ions and determination(a) of the hydrolysis product formed from 1-methylhydantoin with N-carbamoylsarcosinamidohydrolase with formation of sarcosine and detection of the sarcosine with sarcosine oxidase or sarcosine dehydrogenase or(b) of the simultaneously formed nucleoside diphosphate.Type: GrantFiled: February 25, 1985Date of Patent: March 28, 1989Assignee: Boehringer Mannheim GmbHInventors: Joachim Siedel, Rolf Deeg, Albert Roder, Joachim Ziegenhorn, Hans Mollering, Helmgard Gauhl
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Patent number: 4742159Abstract: The present invention provides a process for the preparation of digitalis antibodies in which appropriate mammals are immunized with a digoxin bound to protein, the animal serum is obtained, the immune globulin-containing protein fraction is separated in known manner, the immunologically-active globulins are adsorbed on an immunologically-active column and separated from the other proteins, the antibodies are again eluted from the column and the Fab fractions are split with papain and purified, wherein, as immunologically-active adsorbent, there is used an inorganic matrix of large surface area to which digitoxin aldehyde is bound via a spacer which cannot be split with papain and the splitting off of the Fab fraction from the antibodies is carried out on the matrix.The present invention also provides digitalis antibodies obtained by this process, which antibodies can be used for the therapy of digitalis intoxications and for preparing immunological reagents for the determination of digitalis glycosides.Type: GrantFiled: December 19, 1983Date of Patent: May 3, 1988Assignee: Boehringer Mannheim GmbHInventors: Hans-Georg Batz, Herbert Jungfer, Helmut Lenz, Albert Roder
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Patent number: 4666832Abstract: The present invention provides a new pyruvate oxidase which decarboxylates pyruvate with the formation of hydrogen peroxide, characterized in that it is active without the addition of FAD, TPP and divalent metal ions.The present invention also provides a process for preparing this new enzyme and a reagent containing it.Type: GrantFiled: February 27, 1984Date of Patent: May 19, 1987Assignee: Boehringer Mannheim GmbHInventors: Erich Elstner, Karl-Heinz Schleifer, Friedrich Gotz, Barbara Sedewitz, Albert Roder, Hans Mollering, Hans Seidel
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Patent number: 4645739Abstract: The present invention provides a process for the determination of N-carbamoylsarcosine, wherein a sample solution containing N-carbamoylsarcosine is reacted with N-carbamoylsarcosine-amidohydrolase to give sarcosine, which is then determined.The present invention also provides the enzyme N-carbamoylsarcosine-amidohydrolase, a process for obtaining it and a reagent containing it.Type: GrantFiled: December 16, 1983Date of Patent: February 24, 1987Assignee: Boehringer Mannheim GmbHInventors: Rolf Deeg, Albert Roder, Joachim Siedel, Helmgard Gauhl, Joachim Ziegenhorn
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Patent number: 4550079Abstract: A L(+)-tartrate dehydrogenase with L(+)-tartrate dehydrogenase and D(+)-malate dehydrogenase (decarboxylating) activity, which can be obtained from Rhodopseudomonas sphaeroides, and also a process for the production thereof.Furthermore, a process and a reagent for the determination of L(+)-tartrate and of D(+)-malate.Type: GrantFiled: March 4, 1983Date of Patent: October 29, 1985Assignee: Boehringer Mannheim GmbHInventors: Albert Roder, Hans Seidel, Friedrich Giffhorn
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Patent number: 4544630Abstract: The present invention provides a process for the specific determination of the cholesterol of the LDL fraction in the presence of the HDL fraction of the lipoproteins of serum by the action of cholesterol esterase for the liberation of the cholesterol and oxidation of the liberated cholesterol with cholesterol oxidase and oxygen with the formation of hydrogen peroxide and cholestenone and kinetic measurement of the change of one of the reaction components of the oxidase reaction, especially the formation of hydrogen peroxide, wherein the measurement is carried out in a predetermined period of time, the reaction solution having a tenside concentration of 0.01 to 1.5 mmol/liter, a cholesterol esterase concentration of 0.1 to 30 U/ml. and a pH value of 6.5 to 8.0.The present invention also provides a reagent for carrying out this process.Type: GrantFiled: February 22, 1983Date of Patent: October 1, 1985Assignee: Boehringer Mannheim GmbHInventors: Joachim Ziegenhorn, Albert Roder, Knut Bartl, Gunter Wehmeyer
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Patent number: 4416983Abstract: The present invention provides a process for the determination of NAD(P)H or of salicylate, wherein, in a NAD(P)H-dependent reaction, salicylate is decarboxylated by salicylate hydroxylase and a colored material is formed from the decarboxylation product in the presence of tyrosinase by oxidative coupling with an appropriate colored material component, the colored material formed then being determined photometrically.The present invention also provides a reagent for the determination of NADH or NADPH, wherein it contains salicylate, a chromogenic hydrazone or amine, salicylate hydroxylase, tyrosinase and buffer, as well as a reagent for the determination of salicylate, wherein it contains NAD(P)H, a chromogenic hydrazone or amine, salicylate hydroxylase, tyrosinase and buffer.Type: GrantFiled: December 7, 1981Date of Patent: November 22, 1983Assignee: Boehringer Mannheim GmbHInventors: Albert Roder, Joachim Siedel, Hans Mollering, Hans Seidel, Helmgard Gauhl
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Patent number: 4409328Abstract: For the determination of glycerol, the latter is incubated with galactose oxidase in an aqueous medium in the presence of oxygen and either the oxygen consumption or the amount of H.sub.2 O.sub.2 or glyceraldehyde that is formed is determined. A reagent suitable for this purpose consists of galactose oxidase and a system for the determination of H.sub.2 O.sub.2 or a system for the determination of glyceraldehyde. It can additionally contain an agent for the saponification of esterified glycerol.Type: GrantFiled: September 16, 1981Date of Patent: October 11, 1983Assignee: Boehringer Mannheim GmbHInventors: Joachim Ziegenhorn, Knut Bartl, Albert Roder, Gunter Lang, Hans Mollering, Ulrich Nagele
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Patent number: 4399218Abstract: For the determination of glycerin by oxidation with oxygen in the presence of glycerinoxidase and measurement of the oxygen consumption or of the H.sub.2 O.sub.2 formation, a glycerinoxidase from Aspergillus spec. DSM 1729 is used. A reagent suitable for this method consists of glycerinoxidase from Aspergillus spec. DSM 1729 and a system for the determination of H.sub.2 O.sub.2 and contains additionally, if desired, an agent for the saponification of esterified glycerin.Type: GrantFiled: January 23, 1981Date of Patent: August 16, 1983Assignee: Boehringer Mannheim GmbHInventors: Helmgard Gauhl, Hans Seidel, Gunter Lang, Albert Roder, Joachim Ziegenhorn
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Patent number: 4368261Abstract: A method for the determination of triglycerides by ester cleavage utilizing lipase and optionally esterase with the formation of fatty acids and glycerol, phosphorylation of the glycerol with adnosine triphosphate in the presence of glycerol kinase with the formation of glycerol-1-phosphate and adenosine diphosphate and determination of one of the latter two products. The glycerol kinase used is from Bacillus stearothermophilis and acts in combination with at least one activator selected from the group consisting of detergents, phenol derivatives and aniline derivative.Type: GrantFiled: December 5, 1980Date of Patent: January 11, 1983Assignee: Boehringer Mannheim GmbHInventors: Sigmar Klose, Albert Roder, Walter Schneider
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Patent number: 4343897Abstract: A reconstitutable dry reagent for the turbidimetric determination of lipase, which reagent forms an emulsion upon adding water and comprises substrate oil, protective colloid, emulsifier and activator; and the process for making said dry reagent comprising preparing an aqueous emulsion of a triglyceride containing bile acid salt, colipase, at least 10% by weight of protective colloid, and at least a part of a preserving agent, lyophilizing the emulsion, mixing the lyophilizate with a buffer substance and urea, and recovering said dry reagent.Type: GrantFiled: January 28, 1980Date of Patent: August 10, 1982Assignee: Boehringer Mannheim GmbHInventors: Ulrich Neumann, Karl-Wolfgang Knitsch, Joachim Ziegenhorn, Albert Roder, Werner Zwez, Werner Kramer
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Patent number: 4229529Abstract: Formate, or compounds convertible into formate, is determined by reacting same in the presence of formate dehydrogenase (FDH) and of a hydrogen acceptor, e.g., NAD, wherein the formate dehydrogenase is from Candida boidinii DSM 941, to result in stoichiometric reactions. The process can be used for the removal of formate from solutions containing same.Type: GrantFiled: February 23, 1978Date of Patent: October 21, 1980Assignee: Boehringer Mannheim GmbHInventors: Gerhard Michal, Rolf Laube, Albert Roder, Walter Schneider
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Patent number: 4188465Abstract: Stabilized urease compositions are provided comprising urease and, as a stabilizing agent, a mixture of glutathione, ethylenediamine-tetraacetic acid, and citrate, preferably in a weight ratio of 1:0.5-5:0.5-3:0.5-3.Type: GrantFiled: March 25, 1977Date of Patent: February 12, 1980Assignee: Boehringer Mannheim GmbHInventors: Walter Schneider, Albert Roder, Hans Mollering, Ingeborg Gutmann
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Patent number: 4186052Abstract: A novel peroxidase for reduced nicotinamide-adenine-dinucleotide is provided which peroxidase oxidizes reduced nicotinamide-adenine-dinucleotide with hydrogen peroxide to give nicotinamide-adenine-dinucleotide and water and is characterized by a Michaelis constant K.sub.Mto hydrogen peroxide of 2.8.times.10.sup.-5 M andto reduced nicotinamide-adenine-dinucleotide of 1.7.times.10.sup.-5 M,measured at 25.degree. C. in 0.2M tris buffer of pH 6.0, containing 0.1M potassium acetate. The peroxidase can be prepared by liberating the enzyme from Streptococcus faecalis ATCC 8043 by digestion or by treatment with a surface-active agent and isolating same from the enzyme solution obtained. Hydrogen peroxide is determined in a simple reaction or in a coupled reaction with a specific oxidase by contacting the said peroxidase with the H.sub.2 O.sub.2 producing reaction mixture at a pH of from 6.0 to 9.0 and measuring the change of extinction as a measure of H.sub.2 O.sub.Type: GrantFiled: June 29, 1977Date of Patent: January 29, 1980Assignee: Boehringer Mannheim GmbHInventors: Albert Roder, Hans Mollering, Wolfgang Gruber, Klaus Beaucamp, Hans Seidel, Peter Stahl, Detlef von Hoerschelmann
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Patent number: 3951744Abstract: NADP-dependent dehydrogenases are separated and purified by subjecting mixtures containing it to affinity chromatography using as the affinity chromatography material a nucleotide with a phosphate group in the 2'- or 3'-position, bound to a solid carrier.Type: GrantFiled: July 16, 1974Date of Patent: April 20, 1976Assignee: Boehringer Mannheim G.m.b.H.Inventors: Albert Roder, Klaus Beaucamp, Gunter Weimann, Walter Schneider, Klaus Muhlegger