Abstract: The present invention generally relates to inhibitors of DUOX1. In some aspects, an inhibitor may be applied to a subject having or being at risk for asthma or other conditions. The inhibitor may be applied by various techniques, such as pulmonary or topical delivery. In some embodiments, the inhibitor may include a peptide or other moiety having a reactive electrophile. The reactive electrophile can target cysteine or other residues within DUOX1 to inhibit its activity, e.g., by covalently binding to the residue. Other non-limiting examples of suitable inhibitors include hydroxynonenal, curcumin, sulforaphane, cinnamaldehyde, dimethyl fumarate, or phenyl vinyl sulfonate. Other aspects of the invention are generally directed to methods of making or using such inhibitors, kits involving such inhibitors, devices or formulations containing such inhibitors, or the like.

Abstract: The present invention generally relates to inhibitors of DUOX1. In some aspects, an inhibitor may be applied to a subject having or being at risk for asthma or other conditions. The inhibitor may be applied by various techniques, such as pulmonary or topical delivery. In some embodiments, the inhibitor may include a peptide or other moiety having a reactive electrophile. The reactive electrophile can target cysteine or other residues within DUOX1 to inhibit its activity, e.g., by covalently binding to the residue. Other non-limiting examples of suitable inhibitors include hydroxynonenal, curcumin, sulforaphane, cinnamaldehyde, dimethyl fumarate, or phenyl vinyl sulfonate. Other aspects of the invention are generally directed to methods of making or using such inhibitors, kits involving such inhibitors, devices or formulations containing such inhibitors, or the like.

Abstract: A transfer apparatus for transferring an item includes a rail (3) forming an endless track (4) along which the item (100) is transferred, a transporter (41, 42) which is movable along the rail and includes at least one gripper (10) for gripping the item, a driver (7) for moving the transporter along the rail, wherein the transporter includes at least two carriers (8) engaging the rail and located at a distance from each other, neighboring carriers are coupled to each other by a coupler (9), at least one coupler is carrying at least one gripper, the rail includes a first face (12) and a second face (13), each carrier includes a first engage member (14) and a second engage (15) member (15) engaging the first face (12) and a third engage member (16) engaging the second face (13), the engage members of each carrier are positioned relative to each other such that the engage members form a triangle.

Abstract: A transfer apparatus for transferring an item includes a rail (3) forming an endless track (4) along which the item (100) is transferred, a transporter (41, 42) which is movable along the rail and includes at least one gripper (10) for gripping the item, a driver (7) for moving the transporter along the rail, wherein the transporter includes at least two carriers (8) engaging the rail and located at a distance from each other, neighbouring carriers are coupled to each other by a coupler (9), at least one coupler is carrying at least one gripper, the rail includes a first face (12) and a second face (13), each carrier includes a first engage member (14) and a second engage (15) member (15) engaging the first face (12) and a third engage member (16) engaging the second face (13), the engage members of each carrier are positioned relative to each other such that the engage members form a triangle.

Abstract: The present invention, in some aspects, relates to systems and methods for determining oxidized proteins, including nitrosylated proteins such as S-nitrosylated proteins. The systems and methods of the invention can be used in vitro (e.g., in cell or tissue culture) or in vivo. For instance, in some cases, the invention can be used to spatially determine the location and/or concentration of oxidized proteins within cells and/or tissues (e.g., through visual detection). In one set of embodiments, a nitrosylated or otherwise oxidized moiety on a protein may be reacted with a detection entity, which may be, for example, fluorescent, radioactive, electron-dense, able to bind to a signaling entity or a binding partner in order to produce a signal, etc. In some embodiments, other moieties on the protein may be altered or blocked before reaction of the protein with the detection entity.

Abstract: The present invention, in some aspects, relates to systems and methods for determining oxidized proteins, including nitrosylated proteins such as S-nitrosylated proteins. The systems and methods of the invention can be used in vitro (e.g., in cell or tissue culture) or in vivo. For instance, in some cases, the invention can be used to spatially determine the location and/or concentration of oxidized proteins within cells and/or tissues (e.g., through visual detection). In one set of embodiments, a nitrosylated or otherwise oxidized moiety on a protein may be reacted with a detection entity, which may be, for example, fluorescent, radioactive, electron-dense, able to bind to a signaling entity or a binding partner in order to produce a signal, etc. As a specific example, a nitrosothiol moiety on a nitrosylated protein may be reacted with an alkylating agent to form an alkylthio moiety; the alkylthio moiety may include a detection entity or otherwise be able to interact with a signaling entity.