Abstract: Disclosed herein are chimeric receptors and precursors, chimeric antigen receptors and precursors, universal chimeric receptor cell precursors, chimeric antigen receptor T cells, and methods of constructing and using the same.

Abstract: Some aspects provide genetic circuits to engineer complex and adaptive cellular behaviors. Methods of controlling expression of output sequence(s) are also provided herein using genetic circuits that employ catalytically inactive endonucleases.

Abstract: The invention provides a novel approach to facilitate assembly of DNA molecules. This approach utilizes RNA-guided endonucleases, capable of targeting any DNA sequence, which cleave DNA and generate DNA fragments characterized by single stranded overhangs. After annealing of complementary overhangs, DNA fragments are covalently connected, generating a single DNA molecule. In this way, the present invention combines the reliability of classic restriction-ligation techniques, removes all sequence constraints from the desired final DNA molecule, and expands the number of DNA pieces that can be assembled at once.