Abstract: A device, method, and system for the rapid detection of the tuberculosis mycobacterium dispersed in air is provided which employ a breath analysis device consisting of a replaceable mouthpiece unit, a laser module, an optical system, and a detection unit capable of detecting autofluorescence generated by the tuberculosis mycobacterium when irradiated by the laser, a software capable of analyzing and characterizing the acquired autofluorescence signal and distinguishing the spectrum due to tuberculosis mycobacteria from spectra generated by other bacteria and providing to the user of the breath analysis device an analysis and report on the results of the breath analysis test.