Abstract: Expressing guide nucleic acids (e.g., gRNA) from the same oligonucleotide that contains donor sequence permits the high efficiency, simultaneous transformation of a population of cells with both substrates. Using oligonucleotide chip array technology, one can construct thousands of oligonucleotides with customized gRNA and donor sequence in a cost effective manner. In combination, one can efficiently modify endogenous and exogenous genes.

Abstract: A system, method, and device for configuring an optical aiming device for ballistic drop compensation (BDC). The optical aiming device can include a housing with a reticle pane defining a reticle display field viewable by a user and indicating a zero point, the housing further including a plurality of axially spaced lenses and defining an optical path therethrough. In various embodiments the system includes a display device configured to project an image generated from a display, a processor, and a non-transitory computer readable storage medium. The computer readable data storage medium can include instructions executable by the processor to receive a first set of ballistics input data indicating a first type of ammunition, determine a BDC pattern including at least two holdover marks corresponding to at least two ranges for the first type of ammunition, and project the BDC pattern onto the reticle display field.

Abstract: Optical sights for mounting on a firearm are provided, each of the sights having a base defining a barrel end and a rear end; a frame extending upwardly from the base at the barrel end and defining an opening; an optical element received in the opening; and an illumination device operable to display an aiming point on the optical element to generate said aiming point.

Abstract: The present disclosure provides methods and compositions of modulating expression of a target nucleic acid sequence in a cell. The method comprises introducing into the cell a nucleic acid sequence encoding a Cas9 fusion protein and a guide RNA, wherein the Cas9 fusion protein and the guide RNA are expressed and co-localize at a target site and modulate the expression of the target nucleic acid sequence.

Abstract: A system, method, and device for configuring an optical aiming device for ballistic drop compensation (BDC). The optical aiming device can include a housing with a reticle pane defining a reticle display field viewable by a user and indicating a zero point, the housing further including a plurality of axially spaced lenses and defining an optical path therethrough. In various embodiments the system includes a display device configured to project an image generated from a display, a processor, and a non-transitory computer readable storage medium. The computer readable data storage medium can include instructions executable by the processor to receive a first set of ballistics input data indicating a first type of ammunition, determine a BDC pattern including at least two holdover marks corresponding to at least two ranges for the first type of ammunition, and project the BDC pattern onto the reticle display field.

Abstract: The present disclosure provides methods and compositions of modulating expression of a target nucleic acid sequence in a cell. The method comprises introducing into the cell a nucleic acid sequence encoding a Cas9 fusion protein and a guide RNA, wherein the Cas9 fusion protein and the guide RNA are expressed and co-localize at a target site and modulate the expression of the target nucleic acid sequence.

Abstract: A gunsight for aiming a firearm may comprise a display and an eyepiece optic positioned rearward of the display for allowing a user to view the display through the eyepiece optic and a motion sensing module. The eyepiece optic may comprise one or more eyepiece lenses. Circuitry of the gunsight is operatively coupled to the microbolometer and the display. The circuitry may comprise one or more processors and a non-transitory computer readable medium storing one or more instruction sets. In some embodiments, the one or more instruction sets include instructions configured to be executed by the one or more processors to receive a stream of motion signals from the motion sensing module and display an image to the user in response to the stream of motion signals.

Abstract: The present disclosure provides methods and compositions of modulating expression of a target nucleic acid in a eukaryotic cell. The methods include providing to the cell a guide RNA complementary to the target nucleic acid sequence, providing to the cell a fusion protein, wherein the fusion protein comprises a nuclease null Cas9 protein and a transcriptional effector domain, wherein the nuclease null Cas9 protein interacts with the guide RNA and binds to the target nucleic acid sequence in a site specific manner and wherein the transcriptional effector domain modulates expression of the target nucleic acid.

Abstract: A system, method, and device for configuring an optical aiming device for ballistic drop compensation (BDC). The optical aiming device can include a housing with a reticle pane defining a reticle display field viewable by a user and indicating a zero point, the housing further including a plurality of axially spaced lenses and defining an optical path therethrough. In various embodiments the system includes a display device configured to project an image generated from a display, a processor, and a non-transitory computer readable storage medium. The computer readable data storage medium can include instructions executable by the processor to receive a first set of ballistics input data indicating a first type of ammunition, determine a BDC pattern including at least two holdover marks corresponding to at least two ranges for the first type of ammunition, and project the BDC pattern onto the reticle display field.

Abstract: The present invention relates to in vitro genetic manipulation. In particular, it relates to RNA templated genome editing.

Abstract: Optical sights for mounting on a firearm are provided, each of the sights having a base defining a barrel end and a rear end; a frame extending upwardly from the base at the barrel end and defining an opening; an optical element received in the opening; and an illumination device operable to display an aiming point on the optical element to generate said aiming point.

Abstract: The invention relates to a method that allows a set of embryos to be ranked on the basis of ploidy potential and/or pregnancy generation potential, to aid the process of selecting embryos for transfer in an in-vitro fertilisation procedure. The method measures properties or characteristics of the entire blastocyst; extracts characteristics by identifying different cell types, mainly blastocyst structures and patterns, without extracting characteristics of the first cell divisions and the behaviour thereof over time; and predicts the prognosis of pregnancy and/or ploidy (result of genetic study and successful implantation), using micrographs standardised for the management thereof and by means of sequential preprocessing and machine learning algorithms implemented in a computer in order to rank the potential of a set of embryos, to obtain a successful, live, full-term pregnancy.

Abstract: A system, method, and device for configuring an optical aiming device for ballistic drop compensation (BDC). The optical aiming device can include a housing with a reticle pane defining a reticle display field viewable by a user and indicating a zero point, the housing further including a plurality of axially spaced lenses and defining an optical path therethrough. In various embodiments the system includes a display device configured to project an image generated from a display, a processor, and a non-transitory computer readable storage medium. The computer readable data storage medium can include instructions executable by the processor to receive a first set of ballistics input data indicating a first type of ammunition, determine a BDC pattern including at least two holdover marks corresponding to at least two ranges for the first type of ammunition, and project the BDC pattern onto the reticle display field.

Abstract: The present invention relates to high throughput in vitro genetic manipulation. In particular, it relates to scalable CRISPR gene insertions in mammalian cells.

Abstract: An enhanced display reticle for a range finding device, the reticle including a display in communication with a rangefinder and an inclinometer, the display defining a live angle meter and a power meter, the live angle meter including a plurality of incline hashes, the power meter including a plurality of strength hashes; a processor configured to illuminate one or more of the plurality of incline hashes in response to a signal from the inclinometer; illuminate one or more of the plurality of strength hashes in response to a signal received from the laser rangefinder.

Abstract: CRISPR/Cas Systems are provided where a tuned guide RNA is used to discriminate between two protospacer sequences of same length that differ by one nucleotide.

Abstract: The present invention is providing a system based on artificial vision, and artificial intelligence that is capable of assisting the embryologist to select the best spermatozoa to be injected during an ICSI procedure, requiring the selection of a single sperm. It can identify the best spermatozoa from a sample, in real-time, based on their morphological and motility characteristics which are observed under the microscope at magnifications equal to or above 20×. Is an automatic analysis of sequences of images produced by a digital camera attached to a microscope in real-time. It uses computer vision algorithms to automatically detect and track each of the sperms present on every image of a video and compute a number of features related to the morphological characteristics and motility parameters. The features of each sperm are processed and then evaluated using a mathematical model which determines the quality of each sperm and ranks them accordingly.

Abstract: Methods of making mutant Cas9 proteins are described.

Abstract: A nucleic acid construct is provided that encodes two or more or a plurality of spacer sequences separated by restriction endonuclease recognition site. A plurality of such nucleic acid sequences are provided as a library for making guide RNAs for use with CRISPR/Cas systems.