Patents by Inventor Alejandro San Martin

Alejandro San Martin has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Genetically encoded probe for quantification of lactate and methods for quantifying metabolic rates and lactate transport
    Patent number: 10466233
    Abstract: A nanosensor for detecting and quantifying lactate in different types of samples, such as tissues, intra-cellular and subcellular compartments, with high spatial and temporal resolution is disclosed. Methods comprising use of the nanosensor for quantifying the activity of lactate transporters, rates of cellular lactate production and cellular lactate consumption, and rate of mitochondrial pyruvate consumption are also disclosed. Methods for quantifying the transformation in energy metabolism that characterizes cancer cells with single-cell resolution and for detecting interference of candidate drugs with mitochondrial energetics are additionally disclosed.
    Type: Grant
    Filed: April 13, 2012
    Date of Patent: November 5, 2019
    Assignees: CENTRO DE ESTUDIOS CIENTIFICOS DE VALDIVIA, CARNEGIE INSTITUTION OF WASHINGTON
    Inventors: Luis Felipe Barros Olmedo, Alejandro San Martin, Sebastian Ceballo Charpentier, Wolf B. Frommer
  • Genetically encoded probe for quantification of pyruvate concentration and methods of using the same
    Patent number: 10302631
    Abstract: The present invention relates to a new optical tool for detecting and quantifying pyruvate in samples, in tissues and in cellular and subcellular compartments, with high spatial and temporal resolution, this is a Forster Resonance Energy Transfer (FRET)-based pyruvate sensor comprising a bacterial PdhR transcription factor between any suitable donor and acceptor fluorescent proteins moieties. The invention also relates to methods of use of this novel optical tool for the quantification of the activity of pyruvate transporters, for the quantification of the rates of cellular pyruvate production and consumption, and for the direct quantification of the rate of mitochondrial pyruvate consumption in intact cells.
    Type: Grant
    Filed: August 20, 2013
    Date of Patent: May 28, 2019
    Assignee: CENTRO DE ESTUDIOS CIENTIFICOS DE VALDIVIA
    Inventors: Luis Felipe Barros, Sebastian Ceballo, Alejandro San Martin
  • GENETICALLY ENCODED PROBE FOR QUANTIFICATION OF PYRUVATE CONCENTRATION AND METHODS OF USING THE SAME
    Publication number: 20160209402
    Abstract: The present invention relates to a new optical tool for detecting and quantifying pyruvate in samples, in tissues and in cellular and subcellular compartments, with high spatial and temporal resolution, this is a Forster Resonance Energy Transfer (FRET)-based pyruvate sensor comprising a bacterial PdhR transcription factor between any suitable donor and acceptor fluorescent proteins moieties. The invention also relates to methods of use of this novel optical tool for the quantification of the activity of pyruvate transporters, for the quantification of the rates of cellular pyruvate production and consumption, and for the direct quantification of the rate of mitochondrial pyruvate consumption in intact cells.
    Type: Application
    Filed: August 20, 2013
    Publication date: July 21, 2016
    Inventors: Luis Felipe BARROS, Sebastian CEBALLO, Alejandro SAN MARTIN
  • GENETICALLY ENCODED PROBE FOR QUANTIFICATION OF LACTATE AND METHODS FOR QUANTIFYING METABOLIC RATES AND LACTATE TRANSPORT
    Publication number: 20150072375
    Abstract: A nanosensor for detecting and quantifying lactate in different types of samples, such as tissues, intra-cellular and subcellular compartments, with high spatial and temporal resolution is disclosed. Methods comprising use of the nanosensor for quantifying the activity of lactate transporters, rates of cellular lactate production and cellular lactate consumption, and rate of mitochondrial pyruvate consumption are also disclosed. Methods for quantifying the transformation in energy metabolism that characterizes cancer cells with single-cell resolution and for detecting interference of candidate drugs with mitochondrial energetics are additionally disclosed.
    Type: Application
    Filed: April 13, 2012
    Publication date: March 12, 2015
    Applicants: CARNEGIE INSTITUTION OF WASHINGTON, CENTRO DE ESTUDIOS CIENTIFICOS DE VALDIVIA
    Inventors: Luis Felipe Barros Olmedo, Alejandro San Martin, Sebastian Ceballo Charpentier, Wolf B. Frommer