Abstract: Methods are disclosed herein for promoting survival of a donor liver in a recipient subject, wherein the donor liver is homozygous for rs738409:G mutation in the patatin-like phospholipase domain-containing protein 3 (PNPLA3) gene. The method can include administering to the subject glutathione (GSH) and/or a nucleic acid molecule encoding a component of the ferroptosis pathway (such as GPX4).

Abstract: A velocimeter/nephelometer for measuring the three-dimensional velocity and/or size and/or shape of a particle. A set of laser interferometers and a set of photodiode detectors are arranged on a two-dimensional platform. Each laser interferometer produces a laser beam, with the beams intersecting within an inner area of the platform. Two of the laser interferometers produce like-oriented fringe patterns with an angular separation between the propagation direction of their beams of ninety degrees. A third of the laser interferometers produces a beam with the fringe pattern oriented orthogonal to the fringe patterns of the other two laser interferometers. Each detector is positioned and filtered to detect light from an associated laser interferometer, the light having been scattered by a particle as the particle passes through a volume of observation.

Abstract: Methods are disclosed herein for efficiently generating human induced pluripotent stem cells (iPSC) containing a nucleic acid including a doxycycline promoter operably linked to a nucleic acid encoding Cas9. These methods include transfecting a human somatic cell with a nucleic acid molecule comprising a doxycycline promoter operably linked to a nucleic acid encoding a Cas9, and constitutive promoter operably linked to a tetracycline responsive element and inducing the somatic cell to form an iPSC, thereby producing an iPSC that can undergo CRISPR/Cas9-mediated recombination at a high efficiency. The human iPSC, or a cell differentiated therefrom, is cultured in the presence of doxycycline to induce expression of the Cas9. These cells can then be used to target in any gene of interest by introducing nucleic acids encoding sgRNAs. Induced pluripotent stem cells produced by these methods are also disclosed.

Abstract: Methods are disclosed herein for producing human hepatocytes from human induced pluripotent stem cells. Also provided are transgenic rats for the expansion of human hepatocytes, such as those produced using the methods disclosed herein.

Abstract: Disclosed are compositions and methods for treating a liver disease in a subject by increasing transport or retention of HNF4?, a transcriptional factor, into a nucleus of a hepatocyte in the subject. In some embodiments, the method comprises upregulating expression or function of one or more transcription factors selected from the group consisting of PROX1, NR5A2, NROB2, MTF1, SREBP1, EP300, and POM121C, and functional fragments thereof, and/or downregulating expression or function of one or more transcription factors DNAJB 1/F1SP40, ATF6, ATF4, and PERK, and functional fragments thereof.

Abstract: A velocimeter/nephelometer for measuring the three-dimensional velocity and/or size and/or shape of a particle. A set of laser interferometers and a set of photodiode detectors are arranged on a two-dimensional platform. Each laser interferometer produces a laser beam, with the beams intersecting within an inner area of the platform. Two of the laser interferometers produce like-oriented fringe patterns with an angular separation between the propagation direction of their beams of ninety degrees. A third of the laser interferometers produces a beam with the fringe pattern oriented orthogonal to the fringe patterns of the other two laser interferometers. Each detector is positioned and filtered to detect light from an associated laser interferometer, the light having been scattered by a particle as the particle passes through a volume of observation.

Abstract: Methods and apparatuses implement docker containers with an application store involved in deployment of the containers. Implementation of the containers may be performed via remote controlling means, and the containers may be subsequently updated, including firmware updates.

Abstract: One aspect of the invention provides a method for harvesting adult cells from an organ. The method includes perfusing the organ with a perfusate and isolating adult cells from the organ, thereby harvesting the adult cells from the organ. Another aspect of the invention provides a method for rehabilitating an organ. The method includes: dividing the organ into a first portion and a second portion, perfusing the first portion with a decellularization medium, isolating adult cells from the second portion, and recellularizing the first portion with a suspension of the adult cells, thereby rehabilitating the organ.

Abstract: One aspect of the invention provides a method for harvesting adult cells from an organ. The method includes perfusing the organ with a perfusate and isolating adult cells from the organ, thereby harvesting the adult cells from the organ. Another aspect of the invention provides a method for rehabilitating an organ. The method includes: dividing the organ into a first portion and a second portion, perfusing the first portion with a decellularization medium, isolating adult cells from the second portion, and recellularizing the first portion with a suspension of the adult cells, thereby rehabilitating the organ.

Abstract: Injury-specific resistive exoskeleton devices and iris-based mechanisms utilized therein. The devices assist those currently in rehabilitation or those who have lost partial or all motion to specific muscles. The device can be used for directly driving a limb or providing resistance to partially impaired muscles. For example, the device can provide a resistive force during contraction of an affected/injured muscle and release the resistive force during contraction of an unaffected/healthy muscle. The iris-based joint mechanism generally includes a hub, an iris mechanism, and a plurality of teeth disposed therebetween. When the teeth are engaged with the hub, the mechanism is locked and the servo outputs a rotational force that affects rotation of the user's limb. When the teeth are disengaged with the hub, the mechanism is unlocked and the user has full range of motion of the underlying hinge joint.

Abstract: Methods are disclosed herein for producing human hepatocytes from human induced pluripotent stem cells. Also provided are transgenic rats for the expansion of human hepatocytes, such as those produced using the methods disclosed herein.

Abstract: Methods are disclosed herein for efficiently generating human induced pluripotent stem cells (iPSC) containing a nucleic acid including a doxycycline promoter operably linked to a nucleic acid encoding Cas9. These methods include transfecting a human somatic cell with a nucleic acid molecule comprising a doxycycline promoter operably linked to a nucleic acid encoding a Cas9, and constitutive promoter operably linked to a tetracycline responsive element and inducing the somatic cell to form an iPSC, thereby producing an iPSC that can undergo CRISPR/Cas9-mediated recombination at a high efficiency. The human iPSC, or a cell differentiated therefrom, is cultured in the presence of doxycycline to induce expression of the Cas9. These cells can then be used to target in any gene of interest by introducing nucleic acids encoding sgRNAs. Induced pluripotent stem cells produced by these methods are also disclosed.

Abstract: The presently disclosed invention is directed to the discovery that hepatocyte nuclear factor 4 alpha (HNF4?; also known as NR2A1), a transcription factor, reverses hepatocyte dysfunction in an animal model of cirrhosis, resulting in improvement in hepatic function, treatment of cirrhosis, and prolonged survival.

Abstract: Provided herein are methods or making and using whole or partial organ ECM structures comprising an anticoagulant. Also provided are organ structures prepared according to those methods.

Abstract: One aspect of the invention provides a method for harvesting adult cells from an organ. The method includes perfusing the organ with a perfusate and isolating adult cells from the organ, thereby harvesting the adult cells from the organ. Another aspect of the invention provides a method for rehabilitating an organ. The method includes: dividing the organ into a first portion and a second portion, perfusing the first portion with a decellularization medium, isolating adult cells from the second portion, and recellularizing the first portion with a suspension of the adult cells, thereby rehabilitating the organ.

Abstract: The presently disclosed invention is directed to the discovery that hepatocyte nuclear factor 4 alpha (HNF4?; also known as NR2A1), a transcription factor, reverses hepatocyte dysfunction in an animal model of cirrhosis, resulting in improvement in hepatic function, treatment of cirrhosis, and prolonged survival.

Abstract: One aspect of the invention provides a method for harvesting adult cells from an organ. The method includes perfusing the organ with a perfusate and isolating adult cells from the organ, thereby harvesting the adult cells from the organ. Another aspect of the invention provides a method for rehabilitating an organ. The method includes: dividing the organ into a first portion and a second portion, perfusing the first portion with a decellularization medium, isolating adult cells from the second portion, and recellularizing the first portion with a suspension of the adult cells, thereby rehabilitating the organ.

Abstract: A food product based on dehydrated fruit or vegetable, and a method and equipment assembly for its production thereof, and use of said product as a tidbit or snack or as an additional or complementary ingredient in meals or in various food products such as cereals. The food product is comprised of substantially dry clusters of dehydrated and puffed fruit or vegetable pieces having an appealing shape and size, a crunchy texture and a palatable consistence. To produce the product, prepared fruit or vegetable pieces are dehydrated and puffed, the pieces are then homogeneously mixed with a liquid binder suitable for human or animal consumption and formed into clusters by compression-molding; then the clusters are dried to the desired end moisture contents. The main part of the equipment assembly for producing the product is a cluster forming device.