Abstract: A system (200) for supporting a patient organ can include a primary circuit (212b), a secondary circuit (212a), and a controller (214). The primary circuit can include an inlet configured to connect to the patient (54), an outlet configured to connect to the patient, and a primary pump (206a). The secondary circuit can be connected to the primary blood circuit and can include an enclosure (202) configured to support an organ (50) therein in a blood flow, a secondary pump, a gas transfer unit (208), and a secondary sensor (238a). The controller can be configured to operate the gas transfer unit based on the secondary sensor signal. The organ may be a bio-engineered organ. In an example, the organ is a liver and operates to perform functions of a liver in place of or in support of the liver of the patient.

Abstract: Disclosed herein are methods for retention of shape of decellularized tissue or of a portion of an organ can be accomplished through the irradiation of the decellularized tissue or the portion of the organ inside a shaping mold. The enclosure of decellularized tissue or a portion of an organ inside of a mold or other constraining material, such as stainless steel or platinum or polymers such as polytetrafluoroethylene (PTFE) or polycaprolactone (PCL), allows the tissue to take on the shape of the mold or constraint and subsequently retain that shape after it is irradiated. This can result in decellularized extracellular matrix having defined (pre-determined) shapes. The system can include a hollow device which contains the filler or plug. The system may be inserted into the fistula tract and the filler or plug may be deployed by pulling, pushing or otherwise expelling the filler or plug into the tract.

Abstract: Disclosed herein are methods to maintain or inhibit an alteration in volume or shape of hydrated decellularized extracellular matrix from a mammalian organ or tissue or a portion thereof.

Abstract: A system (200) for growing or supporting an organ (50), in particular a biologically engineered organ, comprises: an enclosure (202); a perfusate circuit: a perfusate pump (206); a gas transfer unit (208) and a optionally a gas mixture unit connected to the gas transfer unit; one or more sensors (212a, 212b); an injection system (216) configured to inject nutrients; and a controller (214). The controller can be configured to operate the pump, the gas transfer unit, and the injection system based on sensor signals from the sensor(s) to grow or support the organ in an automated or semi-automated fashion.

Abstract: The disclosure provides for methods of sealing of decellularized and recellularized engineered organ grafts, thereby providing a reinforced (fortified) decellularized and recellularized engineered organ graft.

Abstract: Disclosed herein are compositions and methods to decellularize an isolated organ or portion thereof. Also disclosed herein are compositions and methods for treatment of disease utilizing a decellularized or recellularized organ. Also disclosed herein are methods of improving decellularization and/or recellularization of an isolated organ or portion thereof.

Abstract: A method of extracellular matrix (ECM) particle formation, and compositions produced by the method, are provided.

Abstract: A system for testing and transporting a biologically engineered organ can include a housing, a transportable enclosure, a perfusate circuit, and a pump. The housing can be configured to receive and support a biologically engineered organ therein in a perfusate flow. The housing can include a perfusate inlet and a perfusate outlet to receive the perfusate flow through the housing. The transportable enclosure can surround at least a portion of the housing. The perfusate circuit can be connected to the perfusate inlet and the perfusate outlet and can be configured to transmit perfusate through the system. The pump can be connected to the circuit and can be configured to circulate perfusate through the perfusate circuit.

Abstract: Disclosed herein are methods for retention of shape of decellularized tissue or of a portion of an organ can be accomplished through the irradiation of the decellularized tissue or the portion of the organ inside a shaping mold. The enclosure of decellularized tissue or a portion of an organ inside of a mold or other constraining material, such as stainless steel or platinum or polymers such as polytetrafluoroethylene (PTFE) or polycaprolactone (PCL), allows the tissue to take on the shape of the mold or constraint and subsequently retain that shape after it is irradiated. This can result in decellularized extracellular matrix having defined (pre-determined) shapes. The system can include a hollow device which contains the filler or plug. The system may be inserted into the fistula tract and the filler or plug may be deployed by pulling, pushing or otherwise expelling the filler or plug into the tract.

Abstract: A method to provide an inflated decellularized extracellular matrix of a mammalian organ or a vascularized portion thereof, or a mammalian vascularized tissue or a vascularized portion thereof, an inflated decellularized extracellular matrix of a mammalian organ or a vascularized portion thereof, or a mammalian vascularized tissue or a vascularized portion thereof, and uses thereof, are provided.

Abstract: A method to provide an inflated decellularized extracellular matrix of a mammalian organ or a vascularized portion thereof, or a mammalian vascularized tissue or a vascularized portion thereof, an inflated decellularized extracellular matrix of a mammalian organ or a vascularized portion thereof, or a mammalian vascularized tissue or a vascularized portion thereof, and uses thereof, are provided.