Abstract: The present invention relates to means and methods for protecting proteins and protein-type compounds in industrial and other applications. In particular, the invention provides a composition comprising at least one protein or protein-type compound immobilized at the surface of a solid carrier embedded in a protective material. Further, the present invention relates to methods for producing such a composition and to the use thereof in, for example, therapeutic applications. In particular, the system may be used to immobilize and protect enzymes on the surface of a carrier to generate a biocatalytical composition with increased resistance to various types of stresses.

Abstract: A method of producing nanobiocatalysts includes: suspending a support material in a liquid medium; functionalizing a surface of the support material by mixing the suspended support material with a functionalizer for a first predefined time at a first predefined temperature, wherein a first broth results; applying a protein directly in the first broth after the first predefined time; mixing the protein with the first broth and incubating the protein for a second predefined time at a second predefined temperature, wherein a second broth results; immobilizing the incubated protein onto the functionalized surface of the support material, wherein a third broth results; filtering the third broth; and gathering a retentate of the filtering. By directly processing the first and second broths allows for providing a continuous process and production of the nanobiocatalysts, which can be optimized regarding time, water, chemical and energy consumption. Also, problems related to thixotrophy can be minimized.

Abstract: The present invention relates to means and methods for protecting proteins and protein-type compounds in industrial and other applications. In particular, the invention provides a composition comprising at least one protein or protein-type compound immobilized at the surface of a solid carrier embedded in a protective material. Further, the present invention relates to methods for producing such a composition and to the use thereof in, for example, therapeutic applications. In particular, the system may be used to immobilize and protect enzymes on the surface of a carrier to generate a biocatalytical composition with increased resistance to various types of stresses.

Abstract: A method of producing nanobiocatalysts includes: suspending a support material in a liquid medium; functionalizing a surface of the support material by mixing the suspended support material with a functionalizer for a first predefined time at a first predefined temperature, wherein a first broth results; applying a protein directly in the first broth after the first predefined time; mixing the protein with the first broth and incubating the protein for a second predefined time at a second predefined temperature, wherein a second broth results; immobilizing the incubated protein onto the functionalized surface of the support material, wherein a third broth results; filtering the third broth; and gathering a retentate of the filtering. By directly processing the first and second broths allows for providing a continuous process and production of the nanobiocatalysts, which can be optimized regarding time, water, chemical and energy consumption. Also, problems related to thixotrophy can be minimized.

Abstract: A method for preparation of a molecular recognition element comprising the steps of binding a template to a surface of a carrier material, providing a recognition material to the surface of the carrier material, initiating polymerization of the recognition material on the surface of the carrier material, stopping the polymerization of the recognition material on the surface of the carrier material, and releasing the template from the surface of the carrier material and the polymerized recognition material. The method is characterized as an aim size of individual imprints is predefined, and the polymerization of the recognition material on the surface of the carrier material is stopped when a size of individual imprints of the polymerized recognition material essentially equals the predefined aim size. This method is readily applicable for preparation of a molecular recognition element useful as a drug, catalyst, competitive affinity ligand inhibitor, competitor, agonist, antagonist or diagnostic agent.

Abstract: A method for preparation of a molecular recognition element comprising the steps of binding a template to a surface of a carrier material, providing a recognition material to the surface of the carrier material, initiating polymerization of the recognition material on the surface of the carrier material, stopping the polymerization of the recognition material on the surface of the carrier material, and releasing the template from the surface of the carrier material and the polymerized recognition material. The method is characterized as an aim size of individual imprints is predefined, and the polymerization of the recognition material on the surface of the carrier material is stopped when a size of individual imprints of the polymerized recognition material essentially equals the predefined aim size. This method is readily applicable for preparation of a molecular recognition element useful as a drug, catalyst, competitive affinity ligand inhibitor, competitor, agonist, antagonist or diagnostic agent.