Patents by Inventor Alex T. Nielsen

Alex T. Nielsen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Increased isoprene production using the archaeal lower mevalonate pathway
    Patent number: 9464301
    Abstract: The invention features methods for producing isoprene from cultured cells using a feedback-resistant mevalonate kinase polypeptide, such as an archaeal mevalonate kinase polypeptide. The resulting isoprene compositions may have increased yields and/or purity of isoprene.
    Type: Grant
    Filed: July 3, 2014
    Date of Patent: October 11, 2016
    Assignees: DANISCO US INC., THE GOODYEAR TIRE & RUBBER COMPANY
    Inventors: Zachary Q. Beck, Anthony R. Calabria, Michael C. Miller, Alex T. Nielsen, Dmitrii V. Vaviline
  • Enhanced production of isoprene using host cells having decreased activity
    Patent number: 9388431
    Abstract: This invention relates to recombinant microorganisms capable of producing isoprene and isoprene production with the use of such recombinant microorganism with good efficiency. In this invention, functional activity of the ispA gene is altered to reduce the production of isoprenoid molecules in recombinant cells engineered to produce isoprene or in cells otherwise susceptible to isoprenoid accumulation during fermentation. This decreased ispA gene functional activity enables enhanced synthesis of isoprene in a host microorganism.
    Type: Grant
    Filed: January 9, 2015
    Date of Patent: July 12, 2016
    Assignees: Danisco US Inc., The Goodyear Tire & Rubber Company
    Inventors: Joseph C. McAuliffe, Rachel E. Muir, Alex T. Nielsen, Caroline M. Peres, Dmitrii V. Vaviline, Derek H. Wells
  • Compositions and methods of PGL for the increased production of isoprene
    Patent number: 9353375
    Abstract: Provided herein are improved compositions and methods for the increased production of isoprene. Also provided herein are improved compositions and methods for the increased production of heterologous polypeptides capable of biological activity.
    Type: Grant
    Filed: October 7, 2014
    Date of Patent: May 31, 2016
    Assignees: DANISCO US INC., THE GOODYEAR TIRE & RUBBER COMPANY
    Inventors: Zachary Q. Beck, Marguerite A. Cervin, Alex T. Nielsen, Caroline M. Peres
  • ENHANCED PRODUCTION OF ISOPRENE USING HOST CELLS HAVING DECREASED ISPA ACTIVITY
    Publication number: 20150218590
    Abstract: This invention relates to recombinant microorganisms capable of producing isoprene and isoprene production with the use of such recombinant microorganism with good efficiency. In this invention, functional activity of the ispA gene is altered to reduce the production of isoprenoid molecules in recombinant cells engineered to produce isoprene or in cells otherwise susceptible to isoprenoid accumulation during fermentation. This decreased ispA gene functional activity enables enhanced synthesis of isoprene in a host microorganism.
    Type: Application
    Filed: January 9, 2015
    Publication date: August 6, 2015
    Inventors: Joseph C. MCAULIFFE, Rachel E. Muir, Alex T. Nielsen, Caroline M. Peres, Dmitrii V. Vaviline, Derek H. Wells
  • INCREASED ISOPRENE PRODUCTION USING THE ARCHAEAL LOWER MEVALONATE PATHWAY
    Publication number: 20150203873
    Abstract: The invention features methods for producing isoprene from cultured cells using a feedback-resistant mevalonate kinase polypeptide, such as an archaeal mevalonate kinase polypeptide. The resulting isoprene compositions may have increased yields and/or purity of isoprene.
    Type: Application
    Filed: July 3, 2014
    Publication date: July 23, 2015
    Inventors: Zachary Q. BECK, Anthony R. CALABRIA, Michael C. MILLER, Alex T. NIELSEN, Dmitrii V. VAVILINE
  • COMPOSITIONS AND METHODS OF PGL FOR THE INCREASED PRODUCTION OF ISOPRENE
    Publication number: 20150104853
    Abstract: Provided herein are improved compositions and methods for the increased production of isoprene. Also provided herein are improved compositions and methods for the increased production of heterologous polypeptides capable of biological activity.
    Type: Application
    Filed: October 7, 2014
    Publication date: April 16, 2015
    Inventors: Zachary Q. BECK, Marguerite A. Cervin, Alex T. Nielsen, Caroline M. Peres
  • Enhanced production of isoprene using host cells having decreased ispA activity
    Patent number: 8975051
    Abstract: This invention relates to recombinant microorganisms capable of producing isoprene and isoprene production with the use of such recombinant microorganism with good efficiency. In this invention, functional activity of the ispA gene is altered to reduce the production of isoprenoid molecules in recombinant cells engineered to produce isoprene or in cells otherwise susceptible to isoprenoid accumulation during fermentation. This decreased ispA gene functional activity enables enhanced synthesis of isoprene in a host microorganism.
    Type: Grant
    Filed: December 21, 2012
    Date of Patent: March 10, 2015
    Assignees: Danisco US Inc., The Goodyear Tire & Rubber Company
    Inventors: Joseph C. McAuliffe, Rachel E. Muir, Alex T. Nielsen, Caroline M. Peres, Dmitrii V. Vaviline, Derek H. Wells
  • MEMBRANE BIOREACTOR FOR INCREASED PRODUCTION OF ISOPRENE GAS
    Publication number: 20150031105
    Abstract: The invention provides improved methods for the production of isoprene from biological materials.
    Type: Application
    Filed: June 3, 2014
    Publication date: January 29, 2015
    Inventors: Anthony R. CALABRIA, Gopal K. CHOTANI, Robin FONG, Alex T. NIELSEN, Karl J. SANFORD
  • Use of recombinant PGL for increased production of isoprene
    Patent number: 8889387
    Abstract: Provided herein are improved compositions and methods for the increased production of isoprene. Also provided herein are improved compositions and methods for the increased production of heterologous polypeptides capable of biological activity.
    Type: Grant
    Filed: May 6, 2013
    Date of Patent: November 18, 2014
    Assignees: Danisco US Inc., The Goodyear Tire & Rubber Company
    Inventors: Zachary Q. Beck, Marguerite A. Cervin, Alex T. Nielsen, Caroline M. Peres
  • METHODS FOR INCREASING MICROBIAL PRODUCTION OF ISOPRENE, ISOPRENOIDS, AND ISOPRENOID PRECURSOR MOLECULES USING GLUCOSE AND ACETATE CO-METABOLISM
    Publication number: 20140335576
    Abstract: Provided herein are methods for the increased production of intracellular acetyl-CoA, mevalonate, isoprenoid precursors, isoprene and/or isoprenoids by recombinant microorganisms via co-metabolism of substrates with varied oxidation levels.
    Type: Application
    Filed: May 15, 2014
    Publication date: November 13, 2014
    Applicants: Danisco US Inc., The Goodyear Tire & Rubber Company
    Inventors: Gopal K. CHOTANI, Alex T. NIELSEN, Dmitrii V. VAVILINE
  • Increased isoprene production using the archaeal lower mevalonate pathway
    Patent number: 8815548
    Abstract: The invention features methods for producing isoprene from cultured cells using a feedback-resistant mevalonate kinase polypeptide, such as an archaeal mevalonate kinase polypeptide. The resulting isoprene compositions may have increased yields and/or purity of isoprene.
    Type: Grant
    Filed: December 28, 2012
    Date of Patent: August 26, 2014
    Assignees: Danisco US Inc., The Goodyear Tire & Rubber Company
    Inventors: Zachary Q. Beck, Anthony R. Calabria, Michael C. Miller, Alex T. Nielsen, Dmitrii V. Vaviline
  • Methods of producing isoprene comprising a membrane bioreactor
    Patent number: 8778647
    Abstract: The invention provides improved methods for the production of isoprene from biological materials using a membrane bioreactor to culture isoprene-producing cells.
    Type: Grant
    Filed: December 22, 2010
    Date of Patent: July 15, 2014
    Assignees: Danisco US Inc., The Goodyear Tire & Rubber Company
    Inventors: Anthony R. Calabria, Gopal K. Chotani, Robin Fong, Alex T. Nielsen, Karl J. Sanford
  • COMPOSITIONS AND METHODS OF PGL FOR THE INCREASED PRODUCTION OF ISOPRENE
    Publication number: 20130295633
    Abstract: Provided herein are improved compositions and methods for the increased production of isoprene. Also provided herein are improved compositions and methods for the increased production of heterologous polypeptides capable of biological activity.
    Type: Application
    Filed: May 6, 2013
    Publication date: November 7, 2013
    Inventors: Zachary Q. BECK, Marguerite A. CERVIN, Alex T. NIELSEN, Caroline M. PERES
  • REDUCTION OF CARBON DIOXIDE EMISSION DURING ISOPRENE PRODUCTION BY FERMENTATION
    Publication number: 20130280778
    Abstract: The present invention provides methods for increasing the amount of isoprene produced by cultured cells with only a minimal increase in carbon dioxide emitted, thereby resulting in process having a greater yield of isoprene relative to carbon dioxide. In addition, the present invention provides compositions that include the cultured cells or isoprene produced there from.
    Type: Application
    Filed: May 21, 2013
    Publication date: October 24, 2013
    Inventors: Gopal K. CHOTANI, Alex T. NIELSEN, Karl J. SANFORD
  • INCREASED ISOPRENE PRODUCTION USING THE ARCHAEAL LOWER MEVALONATE PATHWAY
    Publication number: 20130280769
    Abstract: The invention features methods for producing isoprene from cultured cells using a feedback-resistant mevalonate kinase polypeptide, such as an archaeal mevalonate kinase polypeptide. The resulting isoprene compositions may have increased yields and/or purity of isoprene.
    Type: Application
    Filed: December 28, 2012
    Publication date: October 24, 2013
    Inventors: Zachary Q. BECK, Anthony R. CALABRIA, Michael C. MILLER, Alex T. NIELSEN, Dmitrii V. VAVILINE
  • METHODS FOR INCREASING MICROBIAL PRODUCTION OF ISOPRENE, ISOPRENOIDS, AND ISOPRENOID PRECURSOR MOLECULES USING GLUCOSE AND ACETATE CO-METABOLISM
    Publication number: 20130273625
    Abstract: Provided herein are methods for the increased production of intracellular acetyl-CoA, mevalonate, isoprenoid precursors, isoprene and/or isoprenoids by recombinant microorganisms via co-metabolism of substrates with varied oxidation levels.
    Type: Application
    Filed: October 5, 2012
    Publication date: October 17, 2013
    Inventors: Gopal K. CHOTANI, Alex T. NIELSEN, Dmitrii V. VAVILINE
  • Compositions and methods of PGL for the increased production of isoprene
    Patent number: 8455236
    Abstract: Provided herein are improved compositions and methods for the increased production of isoprene. Also provided herein are improved compositions and methods for the increased production of heterologous polypeptides capable of biological activity.
    Type: Grant
    Filed: December 23, 2010
    Date of Patent: June 4, 2013
    Assignee: Danisco US Inc.
    Inventors: Zachary Q. Beck, Marguerite A. Cervin, Alex T. Nielsen, Caroline M. Peres
  • Increased isoprene production using the archaeal lower mevalonate pathway
    Patent number: 8361762
    Abstract: The invention features methods for producing isoprene from cultured cells using a feedback-resistant mevalonate kinase polypeptide, such as an archaeal mevalonate kinase polypeptide. The resulting isoprene compositions may have increased yields and/or purity of isoprene.
    Type: Grant
    Filed: September 15, 2009
    Date of Patent: January 29, 2013
    Assignees: Danisco US Inc., The Goodyear Tire & Rubber Company
    Inventors: Zachary Q. Beck, Anthony R. Calabria, Michael C. Miller, Dmitrii V. Vaviline, Alex T. Nielsen
  • MEMBRANE BIOREACTOR FOR INCREASED PRODUCTION OF ISOPRENE GAS
    Publication number: 20110195472
    Abstract: The invention provides improved methods for the production of isoprene from biological materials.
    Type: Application
    Filed: December 22, 2010
    Publication date: August 11, 2011
    Inventors: Anthony R. CALABRIA, Gopal K. CHOTANI, Robin FONG, Alex T. NIELSEN, Karl J. SANFORD
  • COMPOSITIONS AND METHODS OF PGL FOR THE INCREASED PRODUCTION OF ISOPRENE
    Publication number: 20110159557
    Abstract: Provided herein are improved compositions and methods for the increased production of isoprene. Also provided herein are improved compositions and methods for the increased production of heterologous polypeptides capable of biological activity.
    Type: Application
    Filed: December 23, 2010
    Publication date: June 30, 2011
    Applicant: Danisco US Inc.
    Inventors: Zachary Q. Beck, Marguerite A. Cervin, Alex T. Nielsen, Caroline M. Peres