Abstract: The present invention discloses a method to construct eukaryotic cells having a target sequence in a chromosomal DNA sequence replaced by a replacement sequence of interest comprising: modifying a parent eukaryotic cell with a preference for NHR to provide a eukaryotic cell having an increased HR/NHR ratio as compared to the parent cell, providing two sets of DNA molecules of which the first set comprises DNA molecules each comprising a first non-functional fragment of the replacement sequence of interest flanked at its 5?-side by a DNA sequence substantially homologous to a sequence of the chromosomal DNA flanking the target sequence and the second set comprises DNA molecules each comprising a second non-functional fragment of the DNA replacement sequence of interest overlapping with the first non-functional fragment and flanked at its 3?-side by a DNA sequence substantially homologous to a sequence of the chromosomal DNA flanking the target sequence, wherein the first and second non-functional fragments

Abstract: The disclosure includes a method of and associated system for placing nodes in a wireless local area network (WLAN). The method includes receiving user-specified parameters regarding the network. The parameters can include a layout of a building or other space, and requirements for the WLAN. An algorithm then employs these parameters to automatically create and optimized layout of multiple wireless access points for the WLAN. The method can display the layout and provide various types of information to the user.

Abstract: A method and apparatus are provided for automatically targeting and modifying an Internet ad. In one example, the method includes receiving a page and an ad to be placed on the page, rendering the page into a graphical format, computing descriptive features about the graphical format, mapping the descriptive features to parameters for rendering the ad, and rendering the ad based on the parameters.

Abstract: A comparative sizing method in which at least one well-fitting source item is received having at least one source item property associated therewith and a target item having at least one associated target item property for which a resultant comparative sizing recommendation is being requested based on the at least one source item as specified by its at least one source item property associated therewith. Each source item and the target item is correlated with each database item having associated database item properties in a database based on source-database item matches and target database item matches. The resultant comparative sizing recommendation is determined based on the correlated source-database item matches and target-database item matches.

Abstract: The disclosure includes a method of and associated system for placing nodes (104-109) in a wireless local area network (WLAN). The method includes receiving user-specified parameters regarding the network. The parameters can include a layout of a building or other space, and requirements for the WLAN. An algorithm then employs these parameters to automatically create an optimized layout of multiple wireless access points (104-109) for the WLAN. The method can display the layout and provide various types of information to the user.

Abstract: The invention relates to a cooling system for an internal combustion engine (12) on a vehicle, preferably for an agricultural or industrial commercial vehicle, in particular, for a tractor. The cooling system (10) comprises a high temperature circuit (14) and a low-temperature circuit (20). The high temperature circuit (14) is provided for cooling the internal combustion engine (12) and comprises at least one cooler (18). The low temperature circuit (20) is provided for cooling a charge air cooler (22) and, optionally, an oil cooler (24) and comprises at least one cooler (26). The charge air cooler (22) is embodied with at least two parts or in two stages.

Abstract: The disclosure includes a method of and associated system for placing nodes (104-109) in a wireless local area network (WLAN). The method includes receiving user-specified parameters regarding the network. The parameters can include a layout of a building or other space, and requirements for the WLAN. An algorithm then employs these parameters to automatically create an optimized layout of multiple wireless access points (104-109) for the WLAN. The method can display the layout and provide various types of information to the user.

Abstract: A system and method for providing access to a communication network includes providing a radio node comprising a first set of access point components including a radio component, and providing a physically separated controller node in communication with the radio node. The access point controller comprises a second set of access point components distinct from the first set of access point components, creating a distributed access point. A system controller may also be used to control at least one of the radio node and the controller node. The radio node, the controller node, and the system controller communicate over a communication link, such as a wireless or wired link.

Abstract: Apparatus and method for facilitating a search for gem settings are described. In one embodiment, a computer-readable medium includes a gem search module to facilitate a search for a gem to be included in a jewelry item. The computer-readable medium also includes a gem setting search module to facilitate a search for a gem setting to be included in the jewelry item, and the gem setting search module is configured to provide a visual representation of the jewelry item as being worn.

Abstract: A process for producing collagen sponge which is insoluble but highly swellable in water, the sponge having a velour-like surface and being particularly suited for medical and cosmetic applications.

Abstract: A method is disclosed for making a water-soluble hydrolyzate of a keratinaceous starting material which comprises first subjecting said starting material to acid treatment at a pH of 2 or below and at an elevated temperature above 80.degree. C. to effect mild hydrolysis thereof and then enzymatically degrading said acid-treated material in an aqueous bath in the presence of urea with an alkaline proteinase having an activity optimum in a range between pH 9 and pH 13, the initial pH of the enzymatic treatment being within the pH range optimum for the enzyme employed.

Abstract: A method for converting blood, animal parts, bone waste, or meat scraps into useful products, which method comprises hydrolyzing such a substrate with a proteinase, preferably in the presence of urea, in an aqueous hydrolysis medium at a pH in a region in which the proteinase displays sufficient activity, subsequently inactivating the enzyme, and working up the hydrolyzate.

Abstract: A method for conditioning a collagen-containing raw material to render it adaptable to hot-water extraction of collagen and collagen degradation products therefrom, which comprises incubating said raw material in an aqueous bath containing a neutral or alkaline protease at a pH between 6.5 and 13 in the presence of a member selected from the group consisting of urea, guanidine, or an acid addition salt of quanidine.

Abstract: A process for the preparation of collagen fibers, especially suited for medicinal use, comprising subjecting animal skin, hide or tendons to digestion with a strong alkali until the material has an amide nitrogen content of approximately 0.20 to 0.40 mole per gram, swelling the mass by thorough intermixing with acid, mechanically separating collagen fibers from the mass, de-swelling the collagen fibers with salts or organic solvents, and dehydrating the collagen fibers to approximately 20 to 40 weight % moisture based on the dry weight of the fibers. A plasticizer may be applied to the fibrous mass before dehydration.

Abstract: A water-soluble hydrolyzate of elastin, suitable for cosmetological use, is disclosed as is a method for making a water-soluble elastin hydrolyzate from starting materials containing elastin which comprises first subjecting said starting material to acid treatment at a pH below about 4 and at an elevated temperature and then enzymatically degrading the acid-treated material, while in comminuted form, in an aqueous bath in the presence of urea, with an alkaline proteinase having an activity optimum in a range between pH 9 and pH 13, the initial pH of the enzymatic treatment being within the pH range optimum for the enzyme employed.