Abstract: A chromatography system comprising at least two pumps, a first pump which is connectable or connected with a liquid reservoir for a first fluid, and a second pump which is connectable or connected with a liquid reservoir for a second fluid, wherein the pump outlet lines from the first pump and the second pump are connected with a connection piece and, viewed in the direction of flow, a chromatography column is provided downstream of this connection piece, wherein, viewed in the direction of flow, an addition unit is provided upstream of the connection piece and a mixer switching valve and a mixer switchable by way of the mixer switching valve are provided between the connection piece and chromatography column, wherein the mixer switching valve has at least two switching positions, wherein the mixer is connectable in a first position and the mixer is bypassable in a second position.

Abstract: The present invention relates to a gas-liquid separator for a chromatography system, comprising: (a) a separating region having an inlet nozzle, a baffle unit and a gas distribution unit; (b) a dividing region having a liquid outlet; and (c) a gas discharge region having a gas outlet; wherein the separating region is connected to the dividing region by a separating opening and the distance of the inlet nozzle from the baffle unit is greater than the smallest longitudinal extension of the separating opening and the inlet nozzle is configured such that a gas-liquid stream directed through the inlet nozzle can act on the baffle unit. The present invention further relates to a chromatography system comprising a separator according to the invention and to a chromatography method wherein the separator is used.

Abstract: The present invention relates to a gas-liquid separator for a chromatography system, comprising: a) a separating region having an inlet nozzle, a baffle unit and a gas distribution unit; (b) a dividing region having a liquid outlet; and (c) a gas discharge region having a gas outlet; wherein the separating region is connected to the dividing region by a separating opening and the distance of the inlet nozzle from the baffle unit is greater than the smallest longitudinal extension of the separating opening and the inlet nozzle is configured such that a gas-liquid stream directed through the inlet nozzle can act on the baffle unit. The present invention further relates to a chromatography system comprising a separator according to the invention and to a chromatography method wherein the separator is used.

Abstract: The present invention describes a method of operating a fractionation collector in chromatography, and a chromatograph system for carrying out the method, wherein a solvent composition is pumped into a chromatography column, which contains at least a portion of a first solvent that is liquid under normal conditions and contains a portion of a second solvent that is gaseous at normal conditions, and the composition flowing out of the chromatography column is at least partially introduced into a detector, wherein a composition containing the first solvent, after leaving the chromatography column, is introduced into a collection vessel of the fraction collector in dependence of the detector signal, and the amount of liquid that is introduced into a collecting vessel of the fraction collector is selected in dependence of the portion of said first solvent.

Abstract: A system for pumping a compressible fluid, a chromatography system comprising the system for pumping a compressible fluid, and a chromatography method using the system for pumping a compressible fluid, the system for pumping a compressible fluid comprising at least two pumps including a first pump and a second pump, respective pump outlet lines of which are brought together in a connection piece and are guided out of this connection piece into a common outlet line, wherein the second pump is controllable by a control unit, wherein the control unit is operatively connected to a flowmeter and the pump output of the second pump is controllable as a function of the flow measurement by the control unit.

Abstract: The present invention describes a method of operating a fractionation collector in chromatography, and a chromatograph system for carrying out the method, wherein a solvent composition is pumped into a chromatography column, which contains at least a portion of a first solvent that is liquid under normal conditions and contains a portion of a second solvent that is gaseous at normal conditions, and the composition flowing out of the chromatography column is at least partially introduced into a detector, wherein a composition containing the first solvent, after leaving the chromatography column, is introduced into a collection vessel of the fraction collector in dependence of the detector signal, and the amount of liquid that is introduced into a collecting vessel of the fraction collector is selected in dependence of the portion of said first solvent.

Abstract: The present invention relates to a gas-liquid separator for a chromatography system, comprising: a) a separating region having an inlet nozzle, a baffle unit and a gas distribution unit; (b) a dividing region having a liquid outlet; and (c) a gas discharge region having a gas outlet; wherein the separating region is connected to the dividing region by a separating opening and the distance of the inlet nozzle from the baffle unit is greater than the smallest longitudinal extension of the separating opening and the inlet nozzle is configured such that a gas-liquid stream directed through the inlet nozzle can act on the baffle unit. The present invention further relates to a chromatography system comprising a separator according to the invention and to a chromatography method wherein the separator is used.

Abstract: A pumping system for compressible fluids comprises a first pump having a first pump outlet and a second pump having a second pump outlet. The first pump outlet and the second pump outlet merge in a junction which is in fluid communication with a main outlet. The pumping system further comprises a control to regulate the output pressure of the second pump on the basis of a measurement of the output pressure of the first pump. The corresponding method of operating a pumping system is also disclosed.

Abstract: A gas-liquid separator is provided having a chamber extending in an axial direction (z) and having at least a flow chamber portion extending into a collecting chamber portion. A deflector is arranged in the flow chamber portion and the deflector has a deflector panel extending radially outwardly (r) and in axial direction (z) to form a narrowing flow channel between the deflector and a side wall of the chamber, wherein the deflector panel has a free edge. There is at least one gas outlet shielded by the deflector panel and being arranged axially offset from the free edge.

Abstract: A pumping system for compressible fluids comprises a first pump (20) having a first pump outlet (21) and a second pump (30) having a second pump outlet (31), wherein the first pump outlet (21) and the second pump outlet (31) merge in a junction (40) which is in fluid communication with a main outlet (48). The pumping system further comprises a control (50) to regulate the output pressure (p2) of the second pump (30) on the basis of a measurement of the output pressure (p1) of the first pump (20). The corresponding method of operating a pumping system is also disclosed.

Abstract: A gas-liquid separator is provided having a chamber extending in an axial direction (z) and having at least a flow chamber portion extending into a collecting chamber portion. A deflector is arranged in the flow chamber portion and the deflector has a deflector panel extending radially outwardly (r) and in axial direction (z) to form a narrowing flow channel between the deflector and a side wall of the chamber, wherein the deflector panel has a free edge. There is at least one gas outlet shielded by the deflector panel and being arranged axially offset from the free edge.

Abstract: A gas-liquid separator is provided having a chamber extending in an axial direction (z) and having at least a flow chamber portion extending into a collecting chamber portion. A deflector is arranged in the flow chamber portion and the deflector has a deflector panel extending radially outwardly (r) and in axial direction (z) to form a narrowing flow channel between the deflector and a side wall of the chamber, wherein the deflector panel has a free edge. There is at least one gas outlet shielded by the deflector panel and being arranged axially offset from the free edge.