Abstract: Aspects of the disclosure relate to compositions and methods for multiplexed gene silencing in a cell or subject. In some embodiments, the disclosure provides an isolated nucleic acid or an rAAV encoding a transgene comprising a RNA-guided nuclease (RGN) operably linked to a first promoter, and a second promoter operably linked to a multi guide-RNA (multi-gRNA) expression cassette encoding one or more gRNAs targeting a gene associated with hypercholesterolemia or dyslipidemia. In some embodiments, the disclosure provides methods of treating a subject having hypercholesterolemia or dyslipidemia by administering the compositions.

Abstract: Provided are methods for ride experience enhancements with external services, which can include receiving a request for transportation of a passenger to a service provider, and determining route information corresponding to the request. Some methods described also include providing service information associated with the service provider to the passenger, and receiving at least one requested service from the passenger based on the provided service information. Some methods described also include transmitting the at least one requested service and at least a portion of the route information to the service provider. Systems and computer program products are also provided.

Abstract: Provided are methods for post drop-off passenger assistance, which can include obtaining location data indicative of an environment where an autonomous vehicle is operating, determining a completion parameter indicative of a completion of a ride of the autonomous vehicle, determining, an assistance parameter indicative of an assistance of the autonomous vehicle to a passenger of the autonomous vehicle to be provided after the completion of the ride, and controlling an operation of the autonomous vehicle for assisting the passenger. Some methods described also include obtaining sensor data and controlling operation of the autonomous vehicle based on the sensor data. Systems and computer program products are also provided.

Abstract: Provided herein systems and methods for processing cryopreserved cells without an expansion phase after thawing, including transfecting cells for gene editing purposes and cryopreserving transfected cells. Systems and methods for high-throughput processing of frozen cells are also provided. The systems and methods provided can be used to modify cells, including gene editing, in cell arrays.

Abstract: Provided are methods for completing an errand request prior to fulfilling a ride request. Some methods described include obtaining a ride request from a passenger; obtaining an errand request from the passenger, wherein the errand request comprises performing an activity at a third location prior to pickup of the passenger at the first location; planning a route comprising the third location, the first location, and the second location; navigating the planned route to complete the errand request from the passenger at the third location; and navigating the planned route to transport the passenger from the first location to the second location upon completion of the errand request. Systems and computer program products are also provided.

Abstract: A multi-tubing pinch valve assembly including a receiving space defined between a pinch protrusion and tubing contact body. The multi-tubing pinch valve assembly is moveable between a retracted state and an extended state. In the extended state, multiple tubes disposed in the receiving space are pinched, restricting flow therethrough. In the retracted state, multiple tubes are capable of being loaded into and/or out of the receiving space.

Abstract: Provided herein systems and methods for processing cryopreserved cells without an expansion phase after thawing, including transfecting cells for gene editing purposes and cryopreserving transfected cells. Systems and methods for high-throughput processing of frozen cells are also provided. The systems and methods provided can be used to modify cells, including gene editing, in cell arrays.

Abstract: Disclosed is an improved motion planner that safely and proactively considers worst-case agent behavior by generating a worst-case homotopy for every nominal homotopy. In some embodiments, a method comprises: generating a first set of maneuvers to be performed by a vehicle in a scenario, the first set of maneuvers based on an expected behavior of at least one agent proximate to the vehicle; generating a second set of maneuvers to be performed by the vehicle, the second set of maneuvers based on worst case behavior of the at least one agent proximate to the vehicle; generating a set of candidate trajectories based on the first set of maneuvers and the second set of maneuvers; selecting a trajectory from the set of candidate trajectories; and generating, with the at least one processor, at least one control signal to operate the vehicle based on the selected trajectory.

Abstract: Provided are methods for attribute verification to enable destination which can include obtaining a ride request requesting a ride for an item from a starting location to a destination, obtaining, credential data indicative of one or more credentials indicative of the item, verifying the one or more credentials indicative of the item, and generating, route data indicative of a route indicative of the ride by an autonomous vehicle based on the ride request. Systems and computer program products are also provided.

Abstract: Disclosed is a method including obtaining, using at least one processor, a user destination; obtaining, using the at least one processor, preference data indicative of a user route preference; determining, using the at least one processor, based on the user destination and the preference data, a set of routes towards a set of destinations, wherein at least one route of the set of routes is associated with one or more operational metrics; ranking, using the at least one processor, based on the preference data, the routes of the set of routes; and controlling, using the at least one processor, based on at least one of the ranked routes, navigation of an autonomous vehicle Systems and computer program products are also provided.

Abstract: Provided are methods for ride experience enhancements with external services, which can include receiving a request for transportation of a passenger to a service provider, and determining route information corresponding to the request. Some methods described also include providing service information associated with the service provider to the passenger, and receiving at least one requested service from the passenger based on the provided service information. Some methods described also include transmitting the at least one requested service and at least a portion of the route information to the service provider. Systems and computer program products are also provided.

Abstract: A method that includes obtaining, using at least one processor, sensor data associated with an environment in which an autonomous vehicle (AV) is in operation. The method includes determining, using the at least one processor, an intended action of the AV based on the environment in which the AV is in operation. The method includes determining, using the at least one processor, an action for the AV based on the intended action of the AV and an agent prediction.

Abstract: Provided are methods for completing an errand request prior to fulfilling a ride request. Some methods described include obtaining a ride request from a passenger; obtaining an errand request from the passenger, wherein the errand request comprises performing an activity at a third location prior to pickup of the passenger at the first location; planning a route comprising the third location, the first location, and the second location; navigating the planned route to complete the errand request from the passenger at the third location; and navigating the planned route to transport the passenger from the first location to the second location upon completion of the errand request. Systems and computer program products are also provided.

Abstract: Provided are methods for post drop-off passenger assistance, which can include obtaining location data indicative of an environment where an autonomous vehicle is operating, determining a completion parameter indicative of a completion of a ride of the autonomous vehicle, determining, an assistance parameter indicative of an assistance of the autonomous vehicle to a passenger of the autonomous vehicle to be provided after the completion of the ride, and controlling an operation of the autonomous vehicle for assisting the passenger. Some methods described also include obtaining sensor data and controlling operation of the autonomous vehicle based on the sensor data. Systems and computer program products are also provided.

Abstract: A multi-tubing pinch valve assembly including a receiving space defined between a pinch protrusion and tubing contact body. The multi-tubing pinch valve assembly is moveable between a retracted state and an extended state. In the extended state, multiple tubes disposed in the receiving space are pinched, restricting flow therethrough. In the retracted state, multiple tubes are capable of being loaded into and/or out of the receiving space.

Abstract: Disclosed is a method including obtaining, using at least one processor, a user destination; obtaining, using the at least one processor, preference data indicative of a user route preference; determining, using the at least one processor, based on the user destination and the preference data, a set of routes towards a set of destinations, wherein at least one route of the set of routes is associated with one or more operational metrics; ranking, using the at least one processor, based on the preference data, the routes of the set of routes; and controlling, using the at least one processor, based on at least one of the ranked routes, navigation of an autonomous vehicle Systems and computer program products are also provided.

Abstract: Disclosed herein are cartridges for transfecting cells and/or generating clonal populations of cells comprising: a) a first compartment configured for performing cell transfection, wherein the first compartment comprises a first inlet configured for introduction of a cell sample; b) a second compartment configured for performing cell selection, wherein an inlet of the second compartment is operably coupled to an outlet of the first compartment, and wherein the second compartment further comprises at least one optically-transparent wall and an outlet that is operably coupled to an intermediate cell removal port; and c) a third compartment configured for performing cell expansion, wherein an inlet of the third compartment is operably coupled to the outlet of the second compartment.

Abstract: Systems and methods for accessing account information are provided. For example, an indication to launch an application that may provide account information may be received. A determination may be made regarding whether the indication is an initial interaction with the application. If the indication is an initial interaction, one or more credentials may be received via an interface that may be displayed via the application. If the indication is not the initial interaction, a token may be accessed. A request that may include the credentials or token may then be generated and transmitted such that credentials or token may be used to authenticate a device that includes the application and a user thereof, a new token may be generated, and a response with the new token and/or account information may be transmitted. The account information may then be displayed by an interface of the application.

Abstract: Aspects of the disclosure relate to compositions and methods for multiplexed gene silencing in a cell or subject. In some embodiments, the disclosure provides an isolated nucleic acid or an rAAV encoding a transgene comprising a RNA-guided nuclease (RGN) operably linked to a first promoter, and a second promoter operably linked to a multi guide-RNA (multi-gRNA) expression cassette encoding one or more gRNAs targeting a gene associated with hypercholesterolemia or dyslipidemia. In some embodiments, the disclosure provides methods of treating a subject having hypercholesterolemia or dyslipidemia by administering the compositions.

Abstract: Aspects of the disclosure relate to integration of a transgene packaged into recombinant adeno-associated virus (rAAV) by nuclease-assisted vector integration (NAVI).