Abstract: Methods and systems for extracellular vesicle characterization are provided herein. Embodiments of the methods include inputting measured physical, biological, or chemical aspects of extracted extracellular vesicles. Next, an information architecture that characterizes relationships between biological entities and diseases in humans or other vertebrates is generated. Then, relationships between the measured physical, biological, or chemical aspects of the isolated extracellular vesicles with the information architecture are automatically inferred, thereby characterizing extracellular vesicles.

Abstract: Provided herein is a method of preparation of a therapeutic formulation comprising extracellular vesicles packaged with fenretinide.

Abstract: Methods and systems for extracellular vesicle characterization are provided herein. Embodiments of the methods include inputting measured physical, biological, or chemical aspects of extracted extracellular vesicles. Next, an information architecture that characterizes relationships between biological entities and diseases in humans or other vertebrates is generated. Then, relationships between the measured physical, biological, or chemical aspects of the isolated extracellular vesicles with the information architecture are automatically inferred, thereby characterizing extracellular vesicles.

Abstract: A system for identifying exosome subpopulations and payloads for potential targeted therapeutics comprises a simultaneous extracellular vesicle membrane and enclosed content detecting composition. The extracellular vesicle membrane and enclosed content detecting composition includes: a bead comprising a selective binding agent; an extracellular vesicle membrane protein-specific DNA tag bound to the selective binding agent and comprising a poly-T end; and an extracellular vesicle-derived RNA comprising a poly-A end bound to the membrane protein-specific DNA tag poly-T end. The composition may comprise cDNA bound to the RNA. The selective binding agent may comprise a barcode and a molecular identifier. The selective binding agent may selectively bind the membrane protein identifier DNA present in a droplet. In some embodiments, the bead may be a magnetic bead. In some embodiments, the bead may be a conductive bead.

Abstract: Methods and systems for extracellular vesicle characterization are provided herein. Embodiments of the methods include inputting measured physical, biological, or chemical aspects of extracted extracellular vesicles. Next, an information architecture that characterizes relationships between biological entities and diseases in humans or other vertebrates is generated. Then, relationships between the measured physical, biological, or chemical aspects of the isolated extracellular vesicles with the information architecture are automatically inferred, thereby characterizing extracellular vesicles.