Abstract: The present invention provides methods, systems and kits for performing microwell analysis in sealed small volumes. The microwells of the invention are sealed through the pairing of microwell and bead geometry wherein the diameter of the beads is greater than the diameter of the bottom of the microwells. Loading the beads into the microwells seals samples within an analytical space between the bottom of a nested bead and the bottom of the subject microwell.

Abstract: The invention provides a method of detecting a target polynucleotide in a sample comprising: (a) contacting the sample with a guide polynucleotide that binds to a sequence in the target polynucleotide and a polynucleotide-guided effector protein, wherein the guide polynucleotide and polynucleotide-guided effector protein form a complex with any target polynucleotide present in the sample; (b) contacting the sample with a membrane comprising a transmembrane pore; (c) applying a potential to the membrane; and (d) monitoring for the presence or absence of an effect resulting from the interaction of the complex with the transmembrane pore to determine the presence or absence of the complex, thereby detecting the target polynucleotide in the sample.

Abstract: The invention provides a method of detecting a target polynucleotide in a sample comprising: (a) contacting the sample with a guide polynucleotide that binds to a sequence in the target polynucleotide and a polynucleotide-guided effector protein, wherein the guide polynucleotide and polynucleotide-guided effector protein form a complex with any target polynucleotide present in the sample; (b) contacting the sample with a membrane comprising a transmembrane pore; (c) applying a potential to the membrane; and (d) monitoring for the presence or absence of an effect resulting from the interaction of the complex with the transmembrane pore to determine the presence or absence of the complex, thereby detecting the target polynucleotide in the sample.

Abstract: Accelerated data acquisition using two-dimensional (“2D”) radio frequency (“RF”) pulse segments as virtual receivers for a parallel image reconstruction technique, such as GRAPPA, is provided. Data acquisition is accelerated using segmented RF pulses for excitation, refocusing, or both, and undersampling k-space along a dimension of the RF pulse segments. In this way, parallel image reconstruction techniques, such as GRAPPA, can be adapted to work with a single RF receive coil. By undersampling the data acquisition and finding correlations between the data from different segments, unsampled data can be recovered. This shortens scan times, yielding the advantages of segmented pulses without the formerly required long scans.

Abstract: An automated method for generating a final setup for orthodontic treatment. The method includes receiving a digital 3D model of teeth in an initial state. The method computes a scoring function based upon metrics related to the initial state to generate a corresponding score, perturbs the state of the teeth through translations and rotations of a tooth or set of teeth to generate a perturbed state of the teeth, and updates the state of the teeth based upon the score and the perturbed state of the teeth to generate one or more final setups representing a final state of the teeth after the orthodontic treatment.

Abstract: The invention provides a method of detecting a target polynucleotide in a sample comprising: (a) contacting the sample with a guide polynucleotide that binds to a sequence in the target polynucleotide and a polynucleotide-guided effector protein, wherein the guide polynucleotide and polynucleotide-guided effector protein form a complex with any target polynucleotide present in the sample; (b) contacting the sample with a membrane comprising a transmembrane pore; (c) applying a potential to the membrane; and (d) monitoring for the presence or absence of an effect resulting from the interaction of the complex with the transmembrane pore to determine the presence or absence of the complex, thereby detecting the target polynucleotide in the sample.

Abstract: Accelerated data acquisition using two-dimensional (“2D”) radio frequency (“RF”) pulse segments as virtual receivers for a parallel image reconstruction technique, such as GRAPPA, is provided. Data acquisition is accelerated using segmented RF pulses for excitation, refocusing, or both, and undersampling k-space along a dimension of the RF pulse segments. In this way, parallel image reconstruction techniques, such as GRAPPA, can be adapted to work with a single RF receive coil. By undersampling the data acquisition and finding correlations between the data from different segments, unsampled data can be recovered. This shortens scan times, yielding the advantages of segmented pulses without the formerly required long scans.

Abstract: The invention provides a method of detecting a target polynucleotide in a sample comprising: (a) contacting the sample with a guide polynucleotide that binds to a sequence in the target polynucleotide and a polynucleotide-guided effector protein, wherein the guide polynucleotide and polynucleotide-guided effector protein form a complex with any target polynucleotide present in the sample; (b) contacting the sample with a membrane comprising a transmembrane pore; (c) applying a potential to the membrane; and (d) monitoring for the presence or absence of an effect resulting from the interaction of the complex with the transmembrane pore to determine the presence or absence of the complex, thereby detecting the target polynucleotide in the sample.

Abstract: The invention relates to a new method of characterising a target polynucleotide using a pore. The method involves controlling the formation of secondary structure by the target polynucleotide after the polynucleotide has moved through the pore.