Patents by Inventor Alexander J. MASTROIANNI
Alexander J. MASTROIANNI has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11964275Abstract: Microfluidic devices having an electrowetting configuration and an optimized droplet actuation surface are provided. The devices include a conductive substrate having a dielectric layer, a hydrophobic layer covalently bonded to the dielectric layer, and a first electrode electrically coupled to the dielectric layer and configured to be connected to a voltage source. The microfluidic devices also include a second electrode configured to be connected to the voltage source. The hydrophobic layer features self-associating molecules covalently bonded to a surface of the dielectric layer in a manner that produces a densely-packed monolayer that resists intercalation and or penetration by polar molecules or species.Type: GrantFiled: August 27, 2020Date of Patent: April 23, 2024Assignee: Berkeley Lights, Inc.Inventors: Randall D. Lowe, Jr., Shao Ning Pei, Jian Gong, Alexander J. Mastroianni, Jason M. McEwen, Justin K. Valley
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Publication number: 20230375447Abstract: The present disclosure relates to methods for assaying and controlling micro-objects in a microfluidic device. In situ-generated hydrogel barriers are provided for dividing a microfluidic chamber into areas where assaying a cell may be performed without interference from the presence of the cell itself.Type: ApplicationFiled: March 27, 2023Publication date: November 23, 2023Inventors: Alexander J. MASTROIANNI, Peyton SHIEH, Kellen C. MOBILIA, Eric K. SACKMANN, Ke-Chih LIN, Or GADISH, Patrick N. INGRAM, Eric Chun-Jen SHIUE, Grayson Thomas WAWRZYN, Volker L.S. KURZ, Nathan J. VER HEUL, Randall D. LOWE, JR., Sara TAFOYA
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Patent number: 11801508Abstract: In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces to provide improved or altered performance with biomaterials.Type: GrantFiled: January 29, 2021Date of Patent: October 31, 2023Assignee: Berkeley Lights, Inc.Inventors: Randall D. Lowe, Jr., Alexander J. Mastroianni, Mark P. White, Gregory G. Lavieu, Kristin G. Beaumont
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Publication number: 20230313107Abstract: Cartridge for manufacturing a population of cells suitable for formulation as a cellular therapeutic are disclosed herein, along with systems for operating the cartridges and performing methods to generate the population of cells suitable for formulation as a cellular therapeutic. The population of cells suitable for formulation as a cellular therapeutic can be T-cells, including CAR T-cells. The systems and methods can be largely automated.Type: ApplicationFiled: December 28, 2022Publication date: October 5, 2023Applicant: BERKELEY LIGHTS, INC.Inventors: Andrew W. McFarland, Peter J. Beemiller, Guido K. Stadler, Alexander J. Mastroianni, Joshua J. Cardiel Rivera, Darcy K. Kelly-Greene, Jonathan Cloud Dragon Hubbard, Natalie C. Marks, Long Van Le, Ke-Chih Lin
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Publication number: 20230201827Abstract: In biosciences and related fields, it can be useful to study cells in isolation so that cells having unique and desirable properties can be identified within a heterogenous mixture of cells. Processes and methods disclosed herein provide for encapsulating cells within a microfluidic device and assaying the encapsulated cells. Encapsulation can, among other benefits, facilitate analyses of cells that generate secretions of interest which would otherwise rapidly diffuse away or mix with the secretions of other cells.Type: ApplicationFiled: February 10, 2023Publication date: June 29, 2023Inventors: Volker L.S. KURZ, Jason M. MCEWEN, Kellen C. MOBILIA, Alexander J. MASTROIANNI, Joshua J. CARDIEL RIVERA
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Patent number: 11612890Abstract: In biosciences and related fields, it can be useful to study cells in isolation so that cells having unique and desirable properties can be identified within a heterogenous mixture of cells. Processes and methods disclosed herein provide for encapsulating cells within a microfluidic device and assaying the encapsulated cells. Encapsulation can, among other benefits, facilitate analyses of cells that generate secretions of interest which would otherwise rapidly diffuse away or mix with the secretions of other cells.Type: GrantFiled: October 28, 2021Date of Patent: March 28, 2023Assignee: Berkeley Lights, Inc.Inventors: Volker L. S. Kurz, Jason M. McEwen, Kellen C. Mobilia, Alexander J. Mastroianni, Joshua J. Cardiel Rivera
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Publication number: 20220325240Abstract: Cartridges for manufacturing a population of cells suitable for formulation as a cellular therapeutic are disclosed herein, along with systems and instruments for operating the cartridges and performing methods to generate the population of cells suitable for formulation as a cellular therapeutic. The population of cells suitable for formulation as a cellular therapeutic can be immunological cells, such as T lymphocytes, including endogenous T cells (ETCs), tumor infiltrating lymphocytes (TILs), CAR T-cells, TCR engineered T-cells, or otherwise engineered T-cells. The systems and methods can be largely automated.Type: ApplicationFiled: January 12, 2022Publication date: October 13, 2022Inventors: Andrew W. McFarland, Peter J. Beemiller, Guido K. Stadler, Alexander J. Mastroianni, Joshua J. Cardiel Rivera, Darcy K. Kelly-Greene, Jonathan Cloud Dragon Hubbard, Natalie C. Marks, Long Van Le, Ke-Chih Lin
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Publication number: 20220161255Abstract: In biosciences and related fields, it can be useful to study cells in isolation so that cells having unique and desirable properties can be identified within a heterogenous mixture of cells. Processes and methods disclosed herein provide for encapsulating cells within a microfluidic device and assaying the encapsulated cells. Encapsulation can, among other benefits, facilitate analyses of cells that generate secretions of interest which would otherwise rapidly diffuse away or mix with the secretions of other cells.Type: ApplicationFiled: October 28, 2021Publication date: May 26, 2022Inventors: Volker L.S. KURZ, Jason M. MCEWEN, Kellen C. MOBILIA, Alexander J. MASTROIANNI, Joshua J. CARDIEL RIVERA
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Publication number: 20220033758Abstract: Methods for laser-assisted repositioning of a micro-object and for culturing an attachment-dependent biological cell within a microfluidic device are described herein. Laser illumination is used to controllably create a bubble which repositions the micro-object. Further, methods of culturing an attachment-dependent biological cell are described, where the methods may include laser-assisted repositioning.Type: ApplicationFiled: August 13, 2021Publication date: February 3, 2022Inventors: Eric K. SACKMANN, Nathan J. VER HEUL, Ryan M. COOPER, Alexander J. MASTROIANNI, Annamaria MOCCIARO, Vincent Haw Tien PAI, Randall D. LOWE, JR., Hector D. NEIRA-QUINTERO, Patrick N. INGRAM, Smriti SRIDHAR
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Publication number: 20210291171Abstract: In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces to provide improved or altered performance with biomaterials.Type: ApplicationFiled: January 29, 2021Publication date: September 23, 2021Applicant: Berkeley Lights, Inc.Inventors: Randall D. Lowe, JR., Alexander J. Mastroianni, Mark P. White, Gregory G. Lavieu, Kristin G. Beaumont
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Patent number: 11007520Abstract: In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces to provide improved or altered performance with biomaterials.Type: GrantFiled: November 20, 2018Date of Patent: May 18, 2021Assignee: Berkeley Lights, Inc.Inventors: Randall D. Lowe, Jr., Alexander J. Mastroianni, Mark P. White, Gregory G. Lavieu, Kristin G. Beaumont
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Publication number: 20210114020Abstract: Microfluidic devices having an electrowetting configuration and an optimized droplet actuation surface are provided. The devices include a conductive substrate having a dielectric layer, a hydrophobic layer covalently bonded to the dielectric layer, and a first electrode electrically coupled to the dielectric layer and configured to be connected to a voltage source. The microfluidic devices also include a second electrode configured to be connected to the voltage source. The hydrophobic layer features self-associating molecules covalently bonded to a surface of the dielectric layer in a manner that produces a densely-packed monolayer that resists intercalation and or penetration by polar molecules or species.Type: ApplicationFiled: August 27, 2020Publication date: April 22, 2021Inventors: Randall D. LOWE, Jr., Shao Ning PEI, Jian GONG, Alexander J. MASTROIANNI, Jason M. MCEWEN, Justin K. VALLEY
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Patent number: 10799865Abstract: Microfluidic devices having an electrowetting configuration and an optimized droplet actuation surface are provided. The devices include a conductive substrate having a dielectric layer, a hydrophobic layer covalently bonded to the dielectric layer, and a first electrode electrically coupled to the dielectric layer and configured to be connected to a voltage source. The microfluidic devices also include a second electrode, optionally included in a cover, configured to be connected to the voltage source. The hydrophobic layer features self-associating molecules covalently bonded to a surface of the dielectric layer in a manner that produces a densely-packed monolayer that resists intercalation and or penetration by polar molecules or species.Type: GrantFiled: October 27, 2016Date of Patent: October 13, 2020Assignee: Berkeley Lights, Inc.Inventors: Randall D. Lowe, Jr., Shao Ning Pei, Jian Gong, Alexander J. Mastroianni, Jason M. McEwen, Justin K. Valley
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Publication number: 20200299351Abstract: In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces to activate T Lymphocytes.Type: ApplicationFiled: January 15, 2020Publication date: September 24, 2020Applicant: Berkeley Lights, Inc.Inventors: Peter J. Beemiller, Alexander J. Mastroianni, Shao Ning Pei, Randall D. Lowe, JR., Annamaria Mocciaro, Kevin D. Loutherback, Yelena Bronevetsky, Guido K. Stadler, Andrew W. McFarland, Kevin T. Chapman, Duane Smith, Natalie C. Marks, Amanda L. Goodsell
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Publication number: 20200139362Abstract: Proto-antigen-presenting surfaces and related kits, methods, and uses are provided. The proto-antigen-presenting surface can comprise a plurality of primary activating molecular ligands comprising a major histocompatibility complex (MHC) molecule configured to bind to a T cell receptor (TCR) of a T cell and a plurality of of co-activating molecular ligands each including a TCR co-activating molecule or an adjunct TCR activating molecule, wherein an exchange factor is bound to the MHC molecules. Exchange factors include, e.g., dipeptides such as GL, GF, GR, etc. Proto-antigen-presenting surfaces can be used to rapidly prepare antigen-presenting surfaces comprising one or more peptide antigens of interest by contacting the proto-antigen-presenting surface with one or more peptide antigens so as to displace the exchange factor. As such, the disclosure facilitates rapid evaluation of the immunogenicity of peptide antigens for activating T lymphocytes.Type: ApplicationFiled: October 17, 2019Publication date: May 7, 2020Applicant: Berkeley Lights, Inc.Inventors: Peter J. BEEMILLER, Alexander J. MASTROIANNI, Shao Ning PEI, Randall D. LOWE, Jr., Annamaria MOCCIARO, Kevin D. LOUTHERBACK, Yelena BRONEVETSKY, Guido K. STADLER, Andrew W. MCFARLAND, Kevin T. CHAPMAN, Duane SMITH, Natalie C. MARKS, Amanda L. GOODSELL
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Publication number: 20200123491Abstract: In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects.Type: ApplicationFiled: September 20, 2019Publication date: April 23, 2020Inventors: Peter J. BEEMILLER, Alexander J. MASTROIANNI, Randall D. LOWE, JR., Yelena BRONEVETSKY
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Publication number: 20200115680Abstract: Methods of expanding T lymphocytes in a microfluidic device are provided. The methods can include introducing one or more T lymphocytes into a microfluidic device; contacting the one or more T lymphocytes with an activating agent; and perfusing culture medium through the microfluidic device for a period of time sufficient to allow the one or more T lymphocytes to undergo at least one round of mitotic cell division. The expansion can be non-specific or antigen-specific. T lymphocytes produced according to the disclosed methods are also provided, along with methods of treating cancer in a subject. The methods of treating cancer can include isolating T lymphocytes from a tissue sample obtained from the subject; expanding the isolated T lymphocytes in a microfluidic device; exporting the expanded T lymphocytes from the microfluidic device; and reintroducing the expanded T lymphocytes into the subject.Type: ApplicationFiled: July 19, 2019Publication date: April 16, 2020Applicant: Berkeley Lights, Inc.Inventors: Yelena Bronevetsky, Xiaohua Wang, Peter J. Beemiller, Kristin G. Beaumont, Randall D. Lowe, JR., Alexander J. Mastroianni, Kevin T. Chapman, Natalie C. Marks
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Publication number: 20190275516Abstract: In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces to provide improved or altered performance with biomaterials.Type: ApplicationFiled: November 20, 2018Publication date: September 12, 2019Applicant: Berkeley Lights, Inc.Inventors: Randall D. Lowe, JR., Alexander J. Mastroianni, Mark P. White, Gregory G. Lavieu, Kristin G. Beaumont
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Publication number: 20180135011Abstract: Methods of expanding T lymphocytes in a microfluidic device are provided. The methods can include introducing one or more T lymphocytes into a microfluidic device; contacting the one or more T lymphocytes with an activating agent; and perfusing culture medium through the microfluidic device for a period of time sufficient to allow the one or more T lymphocytes to undergo at least one round of mitotic cell division. The expansion can be non-specific or antigen-specific. T lymphocytes produced according to the disclosed methods are also provided, along with methods of treating cancer in a subject. The methods of treating cancer can include isolating T lymphocytes from a tissue sample obtained from the subject; expanding the isolated T lymphocytes in a microfluidic device; exporting the expanded T lymphocytes from the microfluidic device; and reintroducing the expanded T lymphocytes into the subject.Type: ApplicationFiled: November 2, 2017Publication date: May 17, 2018Inventors: Yelena Bronevetsky, Xiaohua Wang, Peter J. Beemiller, Kristin G. Beaumont, Randall D. Lowe, JR., Alexander J. Mastroianni, Kevin T. Chapman
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Publication number: 20170173580Abstract: Microfluidic devices having an electrowetting configuration and an optimized droplet actuation surface are provided. The devices include a conductive substrate having a dielectric layer, a hydrophobic layer covalently bonded to the dielectric layer, and a first electrode electrically coupled to the dielectric layer and configured to be connected to a voltage source. The microfluidic devices also include a second electrode, optionally included in a cover, configured to be connected to the voltage source. The hydrophobic layer features self-associating molecules covalently bonded to a surface of the dielectric layer in a manner that produces a densely-packed monolayer that resists intercalation and or penetration by polar molecules or species.Type: ApplicationFiled: October 27, 2016Publication date: June 22, 2017Applicant: Berkeley Lights, Inc.Inventors: Randall D. LOWE, JR., Shao Ning PEI, Jian GONG, Alexander J. MASTROIANNI, Jason M. MCEWEN, Justin K. VALLEY