Abstract: The present disclosure provides assay and method for using assay for streamlined assay preparation and testing. These assay are useful for pathogen (e.g., viral or bacterial) detection. The present disclosure also provides assay assemblies and means to prevent or minimize the formation of bubbles when using puncturing elements in fluidic devices or chambers.

Abstract: An expandable device for attachment to a foldable electronic device having first and second surfaces includes a button and a pivotable frame member. The button has a top side, a bottom side, and a coupling portion and is movable between an expanded configuration and a collapsed configuration. The pivotable frame member is operably coupled to the button and includes a device coupling portion that engages a portion of the foldable electronic device. The pivotable frame member is pivotable between a first configuration whereby the button is positioned along at least one of the first surface or the second surface of the foldable electronic device and a second configuration whereby the button is positioned away from the foldable electronic device.