Abstract: The present invention relates to systems and methods for tissue engineering. In particular, the invention is directed toward systems and methods for conditioning tubular biological structures. For example, an aspect of the present invention comprises systems and methods for uncoupling local mechanical parameters (e.g., circumferential stress, shear stress, and axial stress) from global mechanical parameters (e.g. lumen pressure, flow rate of perfusate, and longitudinal stretch of the construct) to control of local mechanical parameters for conditioning a tubular biological construct.