Patents by Inventor Alexander Shenderov
Alexander Shenderov has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9861986Abstract: The invention provides droplet actuators and droplet actuator cassettes including reagent storage capabilities, as well as methods of making and using the droplet actuators and cassettes. The invention also provides continuous flow channel elements and techniques for using electrodes to manipulate droplets in flowing streams. The invention also discloses methods of separating compounds on a droplet actuator. Various other aspects of the invention are also disclosed.Type: GrantFiled: October 3, 2014Date of Patent: January 9, 2018Assignee: Advanced Liquid Logic, Inc.Inventors: Michael G. Pollack, Prasanna Thwar, Vamsee K. Pamula, Vijay Srinivasan, Allen E. Eckhardt, Alexander Shenderov, Dwayne J. Allen
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Publication number: 20170266653Abstract: Methods of concentrating beads in a droplet and/or loading beads on a fluidic device are provided, including among other things, a method of concentrating beads in a droplet, the method comprising: (a) providing a droplet actuator comprising: (i) an interior droplet operations volume; and (ii) a reservoir exterior to the interior volume; (iii) a droplet established in a liquid path extending from the reservoir into the interior volume; (b) providing magnetically responsive beads in the portion of the droplet which is in the reservoir; (c) magnetically attracting the magnetically responsive beads through the liquid path into the portion of the droplet which is in the interior volume; and (d) forming a droplet comprising one or more of the magnetically responsive beads in the interior volume.Type: ApplicationFiled: June 6, 2017Publication date: September 21, 2017Inventors: Michael G. POLLACK, Ramakrishna SISTA, Zhishan HUA, Vamsee K. PAMULA, Arjun SUDARSAN, Vijay SRINIVASAN, Prasanna THWAR, Alexander SHENDEROV, Dwayne ALLEN
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Patent number: 9675972Abstract: Methods of concentrating beads in a droplet and/or loading beads on a fluidic device are provided, including among other things, a method of concentrating beads in a droplet, the method comprising: (a) providing a droplet actuator comprising: (i) an interior droplet operations volume; and (ii) a reservoir exterior to the interior volume; (iii) a droplet established in a liquid path extending from the reservoir into the interior volume; (b) providing magnetically responsive beads in the portion of the droplet which is in the reservoir; (c) magnetically attracting the magnetically responsive beads through the liquid path into the portion of the droplet which is in the interior volume; and (d) forming a droplet comprising one or more of the magnetically responsive beads in the interior volume.Type: GrantFiled: March 9, 2009Date of Patent: June 13, 2017Assignee: ADVANCED LIQUID LOGIC, INC.Inventors: Michael G. Pollack, Ramakrishna Sista, Zhishan Hua, Vamsee K. Pamula, Arjun Sudarsan, Vijay Srinivasan, Prasanna Thwar, Alexander Shenderov, Dwayne Allen
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Patent number: 9511369Abstract: The invention provides a droplet actuator. The droplet actuator may include a base substrate and a top substrate separated to form a gap. The base substrate may include electrodes configured for conducting droplet operations in the gap; and the top substrate may include a glass substrate portion coupled to a non-glass portion, where the non-glass portion may include one or more openings establishing a fluid path extending from an exterior of the droplet actuator and into the gap. The invention also provides related methods of manufacturing the droplet actuator, methods of using the droplet actuator, and methods of loading the droplet actuator.Type: GrantFiled: April 9, 2014Date of Patent: December 6, 2016Assignee: Advanced Liquid Logic, Inc.Inventors: Vijay Srinivasan, Michael G. Pollack, Alexander Shenderov, Zhishan Hua, Arjun Sudarsan
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Patent number: 9452433Abstract: Methods and devices for conducting chemical or biochemical reactions that require multiple reaction temperatures are described. The methods involve moving one or more reaction droplets or reaction volumes through various reaction zones having different temperatures on a microfluidics apparatus. The devices comprise a microfluidics apparatus comprising appropriate actuators capable of moving reaction droplets or reaction volumes through the various reaction zones.Type: GrantFiled: May 29, 2014Date of Patent: September 27, 2016Assignees: Advanced Liquid Logic, Inc., Duke UniversityInventors: Alexander Shenderov, Michael G. Pollack
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Publication number: 20150027889Abstract: The invention provides droplet actuators and droplet actuator cassettes including reagent storage capabilities, as well as methods of making and using the droplet actuators and cassettes. The invention also provides continuous flow channel elements and techniques for using electrodes to manipulate droplets in flowing streams. The invention also discloses methods of separating compounds on a droplet actuator. Various other aspects of the invention are also disclosed.Type: ApplicationFiled: October 3, 2014Publication date: January 29, 2015Applicant: ADVANCED LIQUID LOGIC, INC.Inventors: Michael G. Pollack, Prasanna Thwar, Vamsee K. Pamula, Vijay Srinivasan, Allen E. Eckhardt, Alexander Shenderov, Dwayne J. Allen
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Publication number: 20140329307Abstract: Methods and devices for conducting chemical or biochemical reactions that require multiple reaction temperatures are described. The methods involve moving one or more reaction droplets or reaction volumes through various reaction zones having different temperatures on a microfluidics apparatus. The devices comprise a microfluidics apparatus comprising appropriate actuators capable of moving reaction droplets or reaction volumes through the various reaction zones.Type: ApplicationFiled: May 29, 2014Publication date: November 6, 2014Applicants: DUKE UNIVERSITY, ADVANCED LIQUID LOGIC, INC.Inventors: Alexander Shenderov, Michael G. Pollack
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Patent number: 8852952Abstract: The invention provides droplet actuators and droplet actuator cassettes including reagent storage capabilities, as well as methods of making and using the droplet actuators and cassettes. The invention also provides continuous flow channel elements and techniques for using electrodes to manipulate droplets in flowing streams. The invention also discloses methods of separating compounds on a droplet actuator. Various other aspects of the invention are also disclosed.Type: GrantFiled: May 4, 2009Date of Patent: October 7, 2014Assignee: Advanced Liquid Logic, Inc.Inventors: Michael G. Pollack, Prasanna Thwar, Vamsee K. Pamula, Allen Eckhardt, Alexander Shenderov, Dwayne Allen, Vijay Srinivasan
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Publication number: 20140251807Abstract: A series of microactuators for manipulating small quantities of liquids, and methods of using these for manipulating liquids, are disclosed. The microactuators are based on the phenomenon of electrowetting and contain no moving parts. The force acting on the liquid is a potential-dependent gradient of adhesion energy between the liquid and a solid insulating surface.Type: ApplicationFiled: May 19, 2014Publication date: September 11, 2014Applicant: ADVANCED LIQUID LOGIC, INC.Inventor: Alexander Shenderov
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Publication number: 20140216932Abstract: The invention provides a droplet actuator. The droplet actuator may include a base substrate and a top substrate separated to form a gap. The base substrate may include electrodes configured for conducting droplet operations in the gap; and the top substrate may include a glass substrate portion coupled to a non-glass portion, where the non-glass portion may include one or more openings establishing a fluid path extending from an exterior of the droplet actuator and into the gap. The invention also provides related methods of manufacturing the droplet actuator, methods of using the droplet actuator, and methods of loading the droplet actuator.Type: ApplicationFiled: April 9, 2014Publication date: August 7, 2014Applicant: ADVANCED LIQUID LOGIC, INC.Inventors: Vijay Srinivasan, Michael G. Pollack, Alexander Shenderov, Zhishan Hua, Arjun Sudarsan
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Patent number: 8734629Abstract: A series of microactuators for manipulating small quantities of liquids, and methods of using these for manipulating liquids, are disclosed. The microactuators are based on the phenomenon of electrowetting and contain no moving parts. The force acting on the liquid is a potential-dependent gradient of adhesion energy between the liquid and a solid insulating surface.Type: GrantFiled: May 5, 2011Date of Patent: May 27, 2014Assignee: Advanced Liquid Logic, Inc.Inventor: Alexander Shenderov
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Patent number: 8702938Abstract: The invention provides a droplet actuator comprising a base substrate and a top substrate separated to form a gap, wherein: (a) the base substrate comprises electrodes configured for conducting droplet operations in the gap; and (b) the top substrate comprises a first portion coupled to second portion, where the second portion comprises one or more openings establishing a fluid path extending from an exterior of the droplet actuator and into the gap. The invention also provides related methods of manufacturing the droplet actuator, methods of using the droplet actuator, and methods of loading the droplet actuator.Type: GrantFiled: September 4, 2008Date of Patent: April 22, 2014Assignee: Advanced Liquid Logic, Inc.Inventors: Vijay Srinivasan, Michael G. Pollack, Alexander Shenderov, Zhishan Hua, Arjun Sudarsan
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Patent number: 8460528Abstract: On-board reagent storage, handling, and dispensing apparatuses and methods for droplet actuator systems are provided. In one embodiment, a fluidic analyzer is provided and includes a droplet actuator including a substrate including a plurality of electrodes configured to actuate a droplet and a reagent storage component in selective fluid communication with the droplet actuator and including a reagent configured to combine with a solvent at a time of use. In another embodiment, a method of conducting fluidic analysis is provided and includes storing a reagent within a reagent storage component in fluid communication with a droplet actuator including a plurality of electrodes configured to actuate a droplet and combining the reagent with a solvent at a time for use within the droplet actuator.Type: GrantFiled: October 14, 2008Date of Patent: June 11, 2013Assignee: Advanced Liquid Logic Inc.Inventors: Michael G. Pollack, Alexander Shenderov, Philip Paik, Vijay Srinivasan, Vamsee K. Pamula
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Publication number: 20110104816Abstract: The invention provides droplet actuators and droplet actuator cassettes including reagent storage capabilities, as well as methods of making and using the droplet actuators and cassettes. The invention also provides continuous flow channel elements and techniques for using electrodes to manipulate droplets in flowing streams. The invention also discloses methods of separating compounds on a droplet actuator. Various other aspects of the invention are also disclosed.Type: ApplicationFiled: May 4, 2009Publication date: May 5, 2011Applicant: ADVANCED LIQUID LOGIC, INC.Inventors: Michael G. Pollack, Prasanna Thwar, Vamsee K. Pamula, Vijay Srinivasan, Allen Eckhardt, Alexander Shenderov, Dwayne Allen
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Publication number: 20110104747Abstract: Methods of concentrating beads in a droplet and/or loading beads on a fluidic device are provided, including among other things, a method of concentrating beads in a droplet, the method comprising: (a) providing a droplet actuator comprising: (i) an interior droplet operations volume; and (ii) a reservoir exterior to the interior volume; (iii) a droplet established in a liquid path extending from the reservoir into the interior volume; (b) providing magnetically responsive beads in the portion of the droplet which is in the reservoir; (c) magnetically attracting the magnetically responsive beads through the liquid path into the portion of the droplet which is in the interior volume; and (d) forming a droplet comprising one or more of the magnetically responsive beads in the interior volume.Type: ApplicationFiled: March 9, 2009Publication date: May 5, 2011Applicant: ADVANCED LIQUID LOGIC, INC.Inventors: Michael G. Pollack, Ramakrishna Sista, Zhishan Hua, Vamsee K. Pamula, Arjun Sudarsan, Vijay Srinivasan, Prasanna Thwar, Alexander Shenderov, Dwayne Allen
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Publication number: 20100282609Abstract: On-board reagent storage, handling, and dispensing apparatuses and methods for droplet actuator systems are provided. In one embodiment, a fluidic analyzer is provided and includes a droplet actuator including a substrate including a plurality of electrodes configured to actuate a droplet and a reagent storage component in selective fluid communication with the droplet actuator and including a reagent configured to combine with a solvent at a time of use. In another embodiment, a method of conducting fluidic analysis is provided and includes storing a reagent within a reagent storage component in fluid communication with a droplet actuator including a plurality of electrodes configured to actuate a droplet and combining the reagent with a solvent at a time for use within the droplet actuator.Type: ApplicationFiled: October 14, 2008Publication date: November 11, 2010Applicant: ADVANCED LIQUID LOGIC, INC.Inventors: Michael G. Pollack, Alexander Shenderov, Philip Paik, Vijay Srinivasan, Vamsee K. Pamula
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Publication number: 20100282608Abstract: The invention provides a droplet actuator comprising a base substrate and a top substrate separated to form a gap, wherein: (a) the base substrate comprises electrodes configured for conducting droplet operations in the gap; and (b) the top substrate comprises a first portion coupled to second portion, where the second portion comprises one or more openings establishing a fluid path extending from an exterior of the droplet actuator and into the gap. The invention also provides related methods of manufacturing the droplet actuator, methods of using the droplet actuator, and methods of loading the droplet actuator.Type: ApplicationFiled: September 4, 2008Publication date: November 11, 2010Applicant: ADVANCED LIQUID LOGIC, INC.Inventors: Vijay Srinivasan, Michael G. Pollack, Alexander Shenderov, Zhishan Hua, Arjun Sudarsan
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Publication number: 20100206094Abstract: An apparatus and a method for concentrating a target substance in a liquid has been invented. The apparatus comprises a first reservoir with inlet openings for introducing a fluid comprising a target substance into the first reservoir and a second reservoir separated from the first reservoir by a partition permeable to permit the fluid to traverse the partition while retaining the target substance in the first reservoir.Type: ApplicationFiled: April 23, 2008Publication date: August 19, 2010Applicant: ADVANCED LIQUID LOGIC, INC.Inventor: Alexander Shenderov
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Publication number: 20100130369Abstract: Various methods, such as a method of detecting SNPs, involving (a) introducing onto a droplet actuator a solution comprising genomic DNA, extension oligos and suspension array beads; (b) dispensing on the droplet actuator one bead per droplet; (c) cleaving DNA from the bead in each droplet; (d) amplifying the cleaved DNA; (e) detecting SNP signals and barcode signals from the amplified DNA.Type: ApplicationFiled: March 24, 2008Publication date: May 27, 2010Applicant: ADVANCED LIQUID LOGIC, INC.Inventors: Alexander Shenderov, Richard Kouri, Varvara Grichko
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Publication number: 20100087012Abstract: An aerosol sample collector with an air flow path comprising: (i) at a first segment thereof, a particle charging device, and (i) at a second segment thereof, deflection plates configured to focus particles of a desired charge into a preselected cross-section of the air flow path. The air flow path also includes charged substrates arranged at an outflow portion of the air flow path to collect charged particles on a collection surface of the charged substrates; and an exit path for flowing particles not in the preselected cross-section of the air flow path away from the charged substrates. Related methods are also provided.Type: ApplicationFiled: April 23, 2008Publication date: April 8, 2010Applicant: ADVANCED LIQUID LOGIC, INC.Inventor: Alexander Shenderov