Patents by Inventor Alexander V. Loboda
Alexander V. Loboda has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11967496Abstract: The present invention relates to the high resolution imaging of samples using imaging mass spectrometry (IMS) and to the imaging of biological samples by imaging mass cytometry (IMC™) in which labelling atoms are detected by IMS. LA-ICP-MS (a form of IMS in which the sample is ablated by a laser, the ablated material is then ionised in an inductively coupled plasma before the ions are detected by mass spectrometry) has been used for analysis of various substances, such as mineral analysis of geological samples, analysis of archaeological samples, and imaging of biological substances. However, traditional LA-ICP-MS systems and methods may not provide high resolution. Described herein are methods and systems for high resolution IMS and IMC.Type: GrantFiled: January 21, 2022Date of Patent: April 23, 2024Assignees: University of OttawaInventors: Paul Corkum, Alexander V. Loboda
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Publication number: 20220223398Abstract: The present invention relates to the high resolution imaging of samples using imaging mass spectrometry (IMS) and to the imaging of biological samples by imaging mass cytometry (IMC™) in which labelling atoms are detected by IMS. LA-ICP-MS (a form of IMS in which the sample is ablated by a laser, the ablated material is then ionised in an inductively coupled plasma before the ions are detected by mass spectrometry) has been used for analysis of various substances, such as mineral analysis of geological samples, analysis of archaeological samples, and imaging of biological substances. However, traditional LA-ICP-MS systems and methods may not provide high resolution. Described herein are methods and systems for high resolution IMS and IMC.Type: ApplicationFiled: January 21, 2022Publication date: July 14, 2022Applicant: Fluidigm Canada Inc.Inventors: Paul Corkum, Alexander V. Loboda
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Publication number: 20220178809Abstract: The invention relates to methods and devices for analysis of samples using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The invention provides methods and devices in which individual ablation plumes are distinctively captured and transferred to the ICP, followed by analysis by mass cytometry.Type: ApplicationFiled: July 22, 2021Publication date: June 9, 2022Inventor: Alexander V. Loboda
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Patent number: 11264221Abstract: The present invention relates to the high resolution imaging of samples using imaging mass spectrometry (IMS) and to the imaging of biological samples by imaging mass cytometry (IMCTM) in which labelling atoms are detected by IMS. LA-ICP-MS (a form of IMS in which the sample is ablated by a laser, the ablated material is then ionised in an inductively coupled plasma before the ions are detected by mass spectrometry) has been used for analysis of various substances, such as mineral analysis of geological samples, analysis of archaeological samples, and imaging of biological substances. However, traditional LA-ICP-MS systems and methods may not provide high resolution. Described herein are methods and systems for high resolution IMS and IMC.Type: GrantFiled: June 18, 2019Date of Patent: March 1, 2022Assignee: FLUIDIGM CANADA INC.Inventors: Paul Corkum, Alexander V. Loboda
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Publication number: 20210404968Abstract: We describe in this application the analysis of samples using elemental or mass spectrometry and the analysis of samples, such as biological samples by suspension mass cytometry or imaging mass cytometry and an inductively coupled plasma torch with reverse vortex flow for elemental analysis and a method of operating an ICP torch configured to interface with a spectrometer.Type: ApplicationFiled: October 28, 2019Publication date: December 30, 2021Applicant: Fluidigm Canada Inc.Inventors: Alexander V. Loboda, Adam Carew
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Publication number: 20210373313Abstract: We describe in this application systems and methods for autofocusing in imaging mass spectrometry. The present application describes improvements over current IMS and IMC apparatus and methods through an autofocus component including a plurality of apertures in the autofocus system, such as a plurality of apertures arranged in 2 dimensions. As a plurality of apertures is used, the autofocus system provides redundancy in the event that measurement of focus on the sample from the illuminating radiation passed through one or more of the apertures fails so as to reduce the number of unsuccessful autofocus attempts.Type: ApplicationFiled: September 10, 2019Publication date: December 2, 2021Applicant: Fluidigm Canada Inc.Inventors: DAAF SANDKUIJL, Alexander V. Loboda, ADAM CAREW, KHASHAYAR ASKARPOUR
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Publication number: 20210333173Abstract: This disclosure relates to systems and methods for high speed modulation sample imaging. Disclosed herein are systems and methods for performing imaging mass cytometry, including analysis of labelling atoms by elemental (e.g., atomic) mass spectrometry. Aspects include a sampling system having, and method of using, a femtosecond (fs) laser and/or laser scanning. Alternatively or in addition, aspects include systems and methods for co-registering other imaging modalities with imaging mass cytometry.Type: ApplicationFiled: September 10, 2019Publication date: October 28, 2021Applicant: Fluidigm Canada Inc.Inventors: DAAF SANDKUIJL, Alexander V. Loboda, ADAM CAREW, MINALINI LAKSHMAN
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Publication number: 20210239707Abstract: Embodiments of the present invention relate to reagents and their use for elemental imaging mass spectrometry of biological samples. The embodiments comprising methods for quantifying one or more analytes within a sample, comprising the steps of: (a) providing the sample, wherein the one or more analytes are immobilized to a sample carrier, wherein the sample has been labelled with one or more mass tags comprising one or more labelling atoms, (b) performing mass cytometry on the sample to determine the level of the one or more labelling atoms, wherein the level of the one or more labelling atoms corresponds to the copy number of the one or more analytes.Type: ApplicationFiled: April 26, 2019Publication date: August 5, 2021Applicant: Fluidigm Canada Inc.Inventors: Alexander V. Loboda, Olga Ornatsky, Vladimir Baranov, Nikita Zabinyakov, Anastasia Mavropoulos, Daniel Majonis, Bedilu Allo, Taunia Closson
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Publication number: 20210118661Abstract: The present invention relates to the high resolution imaging of samples using imaging mass spectrometry (IMS) and to the imaging of biological samples by imaging mass cytometry (IMCTM) in which labelling atoms are detected by IMS. LA-ICP-MS (a form of IMS in which the sample is ablated by a laser, the ablated material is then ionised in an inductively coupled plasma before the ions are detected by mass spectrometry) has been used for analysis of various substances, such as mineral analysis of geological samples, analysis of archaeological samples, and imaging of biological substances. However, traditional LA-ICP-MS systems and methods may not provide high resolution. Described herein are methods and systems for high resolution IMS and IMC.Type: ApplicationFiled: June 18, 2019Publication date: April 22, 2021Applicant: Fluidigm Canada Inc.Inventors: Paul Corkum, Alexander V. Loboda
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Patent number: 10847357Abstract: Apparatus and methods for delivering biological samples to an ICP source of a mass cytometer are disclosed. Biological material is disposed on a plurality of discrete sites on a carrier. The plurality of discrete sites are configured to retain biological material and to release the biological material upon application of energy. The carrier is positioned in proximity to a gas conduit and upon release from the discrete sites, the biological material becomes entrained in a gas flow, which delivers discrete portions of biological material through the conduit to the ICP source for analysis by mass cytometry. The apparatus and methods can provide a continuous stream of discrete portions of biological material to a mass cytometer or mass spectrometer.Type: GrantFiled: October 2, 2019Date of Patent: November 24, 2020Assignee: FLUIDIGM CANADA INC.Inventor: Alexander V. Loboda
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Publication number: 20200140800Abstract: Apparatus and methods for delivering biological samples to an ICP source of a mass cytometer are disclosed. Biological material is disposed on a plurality of discrete sites on a carrier. The plurality of discrete sites are configured to retain biological material and to release the biological material upon application of energy. The carrier is positioned in proximity to a gas conduit and upon release from the discrete sites, the biological material becomes entrained in a gas flow, which delivers discrete portions of biological material through the conduit to the ICP source for analysis by mass cytometry. The apparatus and methods can provide a continuous stream of discrete portions of biological material to a mass cytometer or mass spectrometer.Type: ApplicationFiled: October 2, 2019Publication date: May 7, 2020Applicant: Fluidigm Canada Inc.Inventor: Alexander V. Loboda
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Patent number: 10622199Abstract: The invention relates to methods and devices for analysis of samples using laser ablation imaging mass cytometry and mass spectrometry. The invention provides methods and devices in which individual ablation plumes are distinctively captured and rapidly transferred to the ionization system, followed by analysis by mass spectrometry. A transfer conduit can be used to convey ablation plumes to an ionization system. The transfer conduit can include an asymmetric cone. The transfer conduit can be tapered. A flow sacrificing system can be adapted to divert a part of the sheath flow out a sacrificial outlet while the core of the sheath flow containing ablation plumes enters the ionization system.Type: GrantFiled: August 2, 2017Date of Patent: April 14, 2020Assignee: FLUIDIGM CANADA INC.Inventor: Alexander V. Loboda
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Patent number: 10598584Abstract: The inventors have improved mass cytometer to facilitate its use for the analysis of particles.Type: GrantFiled: October 19, 2018Date of Patent: March 24, 2020Assignee: FLUIDIGM CANADA INC.Inventors: Alexander V. Loboda, Dmitry R. Bandura, Vladimir I. Baranov
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Patent number: 10472600Abstract: Apparatus and methods for delivering biological samples to an ICP source of a mass cytometer are disclosed. Biological material is disposed on a plurality of discrete sites on a carrier. The plurality of discrete sites are configured to retain biological material and to release the biological material upon application of energy. The carrier is positioned in proximity to a gas conduit and upon release from the discrete sites, the biological material becomes entrained in a gas flow, which delivers discrete portions of biological material through the conduit to the ICP source for analysis by mass cytometry. The apparatus and methods can provide a continuous stream of discrete portions of biological material to a mass cytometer or mass spectrometer.Type: GrantFiled: April 30, 2018Date of Patent: November 12, 2019Assignee: FLUIDIGM CANADA INC.Inventor: Alexander V. Loboda
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Publication number: 20190180996Abstract: The invention relates to methods and devices for analysis of samples using laser ablation imaging mass cytometry and mass spectrometry. The invention provides methods and devices in which individual ablation plumes are distinctively captured and rapidly transferred to the ionization system, followed by analysis by mass spectrometry. A transfer conduit can be used to convey ablation plumes to an ionization system. The transfer conduit can include an asymmetric cone. The transfer conduit can be tapered. A flow sacrificing system can be adapted to divert a part of the sheath flow out a sacrificial outlet while the core of the sheath flow containing ablation plumes enters the ionization system.Type: ApplicationFiled: August 2, 2017Publication date: June 13, 2019Applicant: Fluidigm Canada Inc.Inventor: Alexander V. LOBODA
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Publication number: 20190120745Abstract: The inventors have improved mass cytometer to facilitate its use for the analysis of particles.Type: ApplicationFiled: October 19, 2018Publication date: April 25, 2019Inventors: Alexander V. Loboda, Dmitry R. Bandura, Vladimir I. Baranov
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Patent number: 10139332Abstract: The inventors have improved mass cytometer to facilitate its use for the analysis of particles.Type: GrantFiled: December 29, 2015Date of Patent: November 27, 2018Assignee: Fluidigm Canada Inc.Inventors: Alexander V. Loboda, Dmitry R. Bandura, Vladimir I. Baranov
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Publication number: 20180312794Abstract: Apparatus and methods for delivering biological samples to an ICP source of a mass cytometer are disclosed. Biological material is disposed on a plurality of discrete sites on a carrier. The plurality of discrete sites are configured to retain biological material and to release the biological material upon application of energy. The carrier is positioned in proximity to a gas conduit and upon release from the discrete sites, the biological material becomes entrained in a gas flow, which delivers discrete portions of biological material through the conduit to the ICP source for analysis by mass cytometry. The apparatus and methods can provide a continuous stream of discrete portions of biological material to a mass cytometer or mass spectrometer.Type: ApplicationFiled: April 30, 2018Publication date: November 1, 2018Applicant: Fluidigm Canada Inc.Inventor: Alexander V. Loboda
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Patent number: 9963667Abstract: Apparatus and methods for delivering biological samples to an ICP source of a mass cytometer are disclosed. Biological material is disposed on a plurality of discrete sites on a carrier. The plurality of discrete sites are configured to retain biological material and to release the biological material upon application of energy. The carrier is positioned in proximity to a gas conduit and upon release from the discrete sites, the biological material becomes entrained in a gas flow, which delivers discrete portions of biological material through the conduit to the ICP source for analysis by mass cytometry. The apparatus and methods can provide a continuous stream of discrete portions of biological material to a mass cytometer or mass spectrometer.Type: GrantFiled: December 31, 2015Date of Patent: May 8, 2018Assignee: Fluidigm Canada Inc.Inventor: Alexander V. Loboda
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Patent number: 9632079Abstract: The invention relates to methods and devices for molecular cytometry. The molecular cytometer is for use in the analysis of molecular tag labeled particles. In the molecular cytometer, volatile molecular tags attached to the particles, such as cells, are released as groups of molecular tags and ionized by the method of soft ionization to produce corresponding groups of molecular ions. The molecular cytometer has two detectors, one to detect the presence of the groups of molecular ions and the other to detect mobility separated molecular ions. The mobility separated molecular ions are synchronized to correspond with the groups of molecular ions.Type: GrantFiled: May 22, 2014Date of Patent: April 25, 2017Assignee: Fluidigm Canada Inc.Inventors: Dmitry R. Bandura, Vladimir I. Baranov, Alexander V. Loboda, Olga Ornatsky, Scott D. Tanner