Abstract: The invention pertains to method for ribosome profiling at a single cell resolution. The method comprises the steps of i) lysing a single cell; ii) digesting the RNA with a ribonuclease, thereby generating an ribosome footprint containing RNA molecules that are protected against digestion; iii) Inactivating the ribonuclease and releasing the RNA molecules from the ribosomes; iv) end repairing the released RNA; v) constructing an RNA library from the end-repaired RNA molecules; vi) size selecting part of the prepared RNA library for fragments having an insert size of about 20-40 nucleotides; vii) sequencing the size selected RNA library; and viii) determining the translatome of the single cell.

Abstract: Modular microfluidic devices, systems and methods for total RNA analyses The invention relates to microfluidic methods of preparing a sequencing library for analyses of total RNA. The invention also relates to modular microfluidic systems for carrying out these methods.

Abstract: The invention relates to methods for processing an RNA sample and allows for single cell sequencing of full length total RNA. The method includes labeling the RNA sample with at least one of a barcode and a unique molecular identifier.

Abstract: The invention provides methods and compositions relating to high definition fluorescence DNA in situ hybridization (HD-FISH). The probes generated using the methods of the invention demonstrate higher resolution and efficacy than conventional DNA FISH probes.