Patents by Inventor Alexander Vlassov

Alexander Vlassov has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20150166992
    Abstract: The invention relates generally to compositions and methods for inhibiting the function of target nucleic acids by sequence specific binding. The compositions and methods can be used for inhibition of micro RNAs and other relatively short non-coding RNAs.
    Type: Application
    Filed: December 9, 2014
    Publication date: June 18, 2015
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: CHRISTOPHER BURNETT, NITIN PURI, SUSAN MAGDALENO, ALEXANDER VLASSOV
  • Publication number: 20150104801
    Abstract: Disclosed are methods, compositions and kits for the isolation of exosomes from biological fluids and tissues. Volume-excluding polymers are used to precipitate exosomes from biological samples thereby allowing exosome isolation by low-speed (benchtop) centrifugation or filtration. Further fractionation of exosomes after precipitation is also described.
    Type: Application
    Filed: October 24, 2014
    Publication date: April 16, 2015
    Inventors: Alexander VLASSOV, Mu LI, Emily ZERINGER, Richard CONRAD
  • Patent number: 8901284
    Abstract: Disclosed are methods, compositions and kits for the isolation of exosomes from biological fluids and tissues. Volume-excluding polymers are used to precipitate exosomes from biological samples thereby allowing exosome isolation by low-speed (benchtop) centrifugation or filtration. Further fractionation of exosomes after precipitation is also described.
    Type: Grant
    Filed: February 26, 2014
    Date of Patent: December 2, 2014
    Assignee: Life Technologies Corporation
    Inventors: Alexander Vlassov, Mu Li, Emily Zeringer, Richard Conrad
  • Publication number: 20140178888
    Abstract: Disclosed are methods, compositions and kits for the isolation of exosomes from biological fluids and tissues. Volume-excluding polymers are used to precipitate exosomes from biological samples thereby allowing exosome isolation by low-speed (benchtop) centrifugation or filtration. Further fractionation of exosomes after precipitation is also described.
    Type: Application
    Filed: February 26, 2014
    Publication date: June 26, 2014
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Alexander VLASSOV, Mu LI, Emily ZERINGER, Richard CONRAD
  • Publication number: 20140094501
    Abstract: Modification formats having modified nucleotides are provided for siRNA. Short interfering RNA having modification formats and modified nucleotides provided herein reduce off-target effects in RNA interference of endogenous genes. Further modification formatted siRNAs are demonstrated to be stabilized to nuclease-rich environments. Unexpectedly, increasing or maintaining strand bias, while necessary to maintain potency for endogenous RNA interference, is not sufficient for reducing off-target effects in cell biology assays.
    Type: Application
    Filed: August 23, 2013
    Publication date: April 3, 2014
    Applicant: Applied Biosystems, LLC
    Inventors: Nitin PURI, Irudaya CHARLES, Susan MAGDALENO, Alexander VLASSOV, Christopher BURNETT
  • Publication number: 20130273544
    Abstract: Disclosed are methods, compositions and kits for the isolation of exosomes from biological fluids and tissues. Volume-excluding polymers are used to precipitate exosomes from biological samples thereby allowing exosome isolation by low-speed (benchtop) centrifugation or filtration. Further fractionation of exosomes after precipitation is also described.
    Type: Application
    Filed: February 12, 2013
    Publication date: October 17, 2013
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Alexander VLASSOV, Mu Li, Emily Zeringer, Richard Conrad
  • Publication number: 20130244327
    Abstract: Modification formats having modified nucleotides are provided for siRNA. Short interfering RNA having modification formats and modified nucleotides provided herein reduce off-target effects in RNA interference of endogenous genes. Further modification formatted siRNAs are demonstrated to be stabilized to nuclease-rich environments. Unexpectedly, increasing or maintaining strand bias, while necessary to maintain potency for endogenous RNA interference, is not sufficient for reducing off-target effects in cell biology assays.
    Type: Application
    Filed: March 1, 2013
    Publication date: September 19, 2013
    Inventors: Nitin PURI, Irudaya CHARLES, Susan MAGDALENO, Alexander VLASSOV, Chris BURNETT
  • Patent number: 8524681
    Abstract: Modification formats having modified nucleotides are provided for siRNA. Short interfering RNA having modification formats and modified nucleotides provided herein reduce off-target effects in RNA interference of endogenous genes. Further modification formatted siRNAs are demonstrated to be stabilized to nuclease-rich environments. Unexpectedly, increasing or maintaining strand bias, while necessary to maintain potency for endogenous RNA interference, is not sufficient for reducing off-target effects in cell biology assays.
    Type: Grant
    Filed: March 19, 2010
    Date of Patent: September 3, 2013
    Assignee: Applied Biosystems, LLC
    Inventors: Nitin Puri, Irudaya Charles, Susan Magdaleno, Alexander Vlassov, Chris Burnett
  • Publication number: 20110251261
    Abstract: The invention relates generally to compositions and methods for inhibiting the function of target nucleic acids by sequence specific binding. The compositions and methods can be used for inhibition of micro RNAs and other relatively short non-coding RNAs.
    Type: Application
    Filed: April 13, 2011
    Publication date: October 13, 2011
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: CHRISTOPHER BURNETT, NITIN PURI, SUSAN MAGDALENO, ALEXANDER VLASSOV
  • Publication number: 20100222414
    Abstract: Modification formats having modified nucleotides are provided for siRNA. Short interfering RNA having modification formats and modified nucleotides provided herein reduce off-target effects in RNA interference of endogenous genes. Further modification formatted siRNAs are demonstrated to be stabilized to nuclease-rich environments. Unexpectedly, increasing or maintaining strand bias, while necessary to maintain potency for endogenous RNA interference, is not sufficient for reducing off-target effects in cell biology assays.
    Type: Application
    Filed: March 19, 2010
    Publication date: September 2, 2010
    Applicant: APPLIED BIOSYSTEMS, LLC
    Inventors: Nitin PURI, Irudaya CHARLES, Susan MAGDALENO, Alexander VLASSOV, Chris BURNETT
  • Publication number: 20070149470
    Abstract: The invention provides methods, compositions, and kits comprising small interfering RNA (shRNA or siRNA) that are useful for inhibition of viral-mediated gene expression. Small interfering RNAs as described herein can be used in methods of treatment of HCV infection. ShRNA and siRNA constructs targetING the internal ribosome entry site (IRES) sequence of HCV are described.
    Type: Application
    Filed: June 1, 2006
    Publication date: June 28, 2007
    Inventors: Roger Kaspar, Heini Ilves, Attila Seyhan, Alexander Vlassov, Brian Johnston
  • Publication number: 20060051789
    Abstract: Methods of preparing gene-specific oligonucleotide libraries are disclosed. In one embodiment a double-stranded RNA corresponding to both sense and antisense strands of mRNA is digested by ribonuclease to produce short RNA fragments. In subsequent ligation steps, flanking oligoribonucleotides of defined sequences may be attached to the 3- and 5-ends of each fragment by RNA ligase (such as T4 RNA ligase). The products of ligation can be reverse transcribed and PCR amplified (RT-PCR) using the oligonucleotides attached to the gene-derived sequences as primer-binding sites. Various methods for incorporating libraries into expression vectors allowing expression of either siRNAs or shRNAs are also disclosed.
    Type: Application
    Filed: July 1, 2005
    Publication date: March 9, 2006
    Inventors: Sergei Kazakov, Alexander Vlassov, Anne Dallas, Attila Seyhan, Levente Egry, Heini Ilves, Roger Kaspar, Brian Johnston