Abstract: The present disclosure provides variant OmpG polypeptides, compositions comprising the OmpG variant polypeptides, and methods for using the variant OmpG polypeptides as nanopores for determining the sequence of single stranded nucleic acids. The variant OmpG nanopores reduce the ionic current noise versus the parental OmpG polypeptide from which they are derived and thereby enable sequencing of polynucleotides with single nucleotide resolution. The reduced ionic current noise also provides for the use of these OmpG nanopore variants in other single molecule sensing applications, e.g., protein sequencing.

Abstract: A proning device includes a conformable structure that adapts to the shape of a subject, surrounded by a shell that imparts a substantially elliptical cross-sectional shape to an exterior of the device to facilitate rotation of the subject within the device when the device is wrapped around the subject. A low-friction interface may also be provided to further facilitate rotation of the subject. The device advantageously reduces staffing needs for proning a subject while facilitating extended proning and periodic re-orientation of the subject. Also disclosed herein are methods for making and using such a proning device.

Abstract: Needle drivers and methods of the present disclosure are generally directed at intracorporeal suture tying using a winding technique. For example, a needle driver may include an elongate shaft having a magnet section between a proximal section and a distal section. A clamp coupled to the distal section may grasp a first end portion of a suture. A needle coupled to a second end portion of the suture may be magnetically secured to the magnet section, and the magnet section may then be rotated to form a loop in the suture. Forming a knot in the suture may include moving the loop over the first end portion of the suture grasped in the clamp. As compared to other intracorporeal suture tying techniques, intracorporeal knot tying carried out using the needle drivers and methods of the present disclosure may reduce the time and complexity associated with laparoscopic procedures.

Abstract: The fastener assemblies, systems, and methods of the present disclosure are generally directed to a first T-fastener and a second T-fastener securable to one another through the use of a suture extending therebetween and deliverable to an anatomical location using a minimally invasive technique. For example, a cannula of a needle assembly may be percutaneously deliverable to a treatment site and, through actuation of a hub of the needle assembly, the first T-fastener and the second T-fastener may be deliverable relative to biological tissue to be fastened at the treatment site. The suture may intracorporeally fasten the first T-fastener and the second T-fastener relative to one another for robust securement biological tissue therebetween. As compared to securement using external fixation, the fastener assemblies, systems, and methods of the present disclosure may facilitate intracorporeally fastening tissue while reducing or eliminating certain requirements associated with postoperative care.

Abstract: An electrocautery device with a retractable suction tube is described. The electrocautery device includes a suction tube assembly that can move between an extended or retracted position using vacuum force generated by a suction source. In some embodiments, the suction tube assembly automatically locks into the extended position when extended such that axial force cannot cause the suction tube assembly to retract. The device may be suitable for use in laparoscopic procedures. The suction tube may be coaxially aligned with the electrocautery tip.

Abstract: The present disclosure provides variant OmpG polypeptides, compositions comprising the OmpG variant polypeptides, and methods for using the variant OmpG polypeptides as nanopores for determining the sequence of single stranded nucleic acids. The variant OmpG nanopores reduce the ionic current noise versus the parental OmpG polypeptide from which they are derived and thereby enable sequencing of polynucleotides with single nucleotide resolution. The reduced ionic current noise also provides for the use of these OmpG nanopore variants in other single molecule sensing applications, e.g., protein sequencing.

Abstract: A method of using a catheter assembly for inserting in a fluid filled space in a body includes providing a main body having a first end portion and a second end portion. The first end portion is positioned within the fluid filled space. The second end portion is adjusted to extend outwardly from the fluid filled space when the first end portion is positioned within the fluid filled space. A catheter tip is connected to the second end portion of the main body. The catheter tip includes a housing having a cavity defined therein and a rotating element positioned within the fluid filled space. The rotating element is rotated within the cavity of the housing to impart movement of the first end portion of the main body within the fluid filled space.

Abstract: A method of using a catheter assembly for inserting in a fluid filled space in a body includes providing a main body having a first end portion and a second end portion. The first end portion is positioned within the fluid filled space. The second end portion is adjusted to extend outwardly from the fluid filled space when the first end portion is positioned within the fluid filled space. A catheter tip is connected to the second end portion of the main body. The catheter tip includes a housing having a cavity defined therein and a rotating element positioned within the fluid filled space. The rotating element is rotated within the cavity of the housing to impart movement of the first end portion of the main body within the fluid filled space.

Abstract: The present disclosure provides variant OmpG polypeptides, compositions comprising the OmpG variant polypeptides, and methods for using the variant OmpG polypeptides as nanopores for determining the sequence of single stranded nucleic acids. The variant OmpG nanopores reduce the ionic current noise versus the parental OmpG polypeptide from which they are derived and thereby enable sequencing of polynucleotides with single nucleotide resolution. The reduced ionic current noise also provides for the use of these OmpG nanopore variants in other single molecule sensing applications, e.g., protein sequencing.

Abstract: The present disclosure provides variant OmpG polypeptides, compositions comprising the OmpG variant polypeptides, and methods for using the variant OmpG polypeptides as nanopores for determining the sequence of single stranded nucleic acids. The variant OmpG nanopores reduce the ionic current noise versus the parental OmpG polypeptide from which they are derived and thereby enable sequencing of polynucleotides with single nucleotide resolution. The reduced ionic current noise also provides for the use of these OmpG nanopore variants in other single molecule sensing applications, e.g., protein sequencing.

Abstract: A method of using a catheter assembly for inserting in a fluid filled space in a body includes providing a main body having a first end portion and a second end portion. The first end portion is positioned within the fluid filled space. The second end portion is adjusted to extend outwardly from the fluid filled space when the first end portion is positioned within the fluid filled space. A catheter tip is connected to the second end portion of the main body. The catheter tip includes a housing having a cavity defined therein and a rotating element positioned within the fluid filled space. The rotating element is rotated within the cavity of the housing to impart movement of the first end portion of the main body within the fluid filled space.

Abstract: A catheter assembly for inserting in a fluid filled space in a body includes a main body having a first end portion and a second end portion. The first end portion is positionable within the fluid filled space and the second end portion is adapted to extend outward from the fluid filled space when the first end portion is positioned within the fluid filled space. The catheter assembly also includes a catheter tip that is connected to the second end portion. The catheter tip includes a housing having a cavity defined therein. The catheter tip also includes a rotating element positioned within the cavity. The rotating element is configured to rotate within the cavity to facilitate movement of the first end portion of the main body within the fluid filled space.