Patents by Inventor Alexandra E. Grier

Alexandra E. Grier has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • LONG POLY(A) PLASMIDS AND METHODS FOR INTRODUCTION OF LONG POLY(A) SEQUENCES INTO THE PLASMID
    Publication number: 20230054471
    Abstract: Disclosed herein are polynucleotides having a plurality of thymine nucleotides and an endonuclease recognition site inserted therein, methods of engineering the polynucleotides having a plurality of thymine nucleotides and an endonuclease recognition site inserted therein, and methods of enhancing transcription, translation, and increasing stability of a polynucleotide.
    Type: Application
    Filed: July 11, 2022
    Publication date: February 23, 2023
    Inventors: Andrew M. Scharenberg, Kyle Jacoby, Alexandra E. Grier
  • Long poly(A) plasmids and methods for introduction of long poly(A) sequences into the plasmid
    Patent number: 11400169
    Abstract: Disclosed herein are polynucleotides having a plurality of thymine nucleotides and an endonuclease recognition site inserted therein, methods of engineering the polynucleotides having a plurality of thymine nucleotides and an endonuclease recognition site inserted therein, and methods of enhancing transcription, translation, and increasing stability of a polynucleotide.
    Type: Grant
    Filed: April 11, 2018
    Date of Patent: August 2, 2022
    Assignee: SEATTLE CHILDREN'S HOSPITAL
    Inventors: Andrew M. Scharenberg, Kyle Jacoby, Alexandra E. Grier
  • ENHANCING ENDONUCLEASE BASED GENE EDITING IN PRIMARY CELLS
    Publication number: 20200377911
    Abstract: Disclosed herein are nuclease-based systems for genome editing and methods of using the system for genome editing. Also, disclosed are approaches to enhance Cas9-mediated gene editing efficiency in primary human cells with minimal toxicity when using adeno-associated virus vectors (AAV) to express the guide RNAs necessary for CRISPR/Cas9-based genome editing in the presence of helper proteins.
    Type: Application
    Filed: January 13, 2020
    Publication date: December 3, 2020
    Inventors: Andrew M. Scharenberg, David J. Rawlings, Michael C. Jensen, Kamila Sabina Gwiazda, Alexandra E. Grier
  • LONG POLY(A) PLASMIDS AND METHODS FOR INTRODUCTION OF LONG POLY(A) SEQUENCES INTO THE PLASMID
    Publication number: 20180272007
    Abstract: Disclosed herein are polynucleotides having a plurality of thymine nucleotides and an endonuclease recognition site inserted therein, methods of engineering the polynucleotides having a plurality of thymine nucleotides and an endonuclease recognition site inserted therein, and methods of enhancing transcription, translation, and increasing stability of a polynucleotide.
    Type: Application
    Filed: April 11, 2018
    Publication date: September 27, 2018
    Inventors: Andrew M. Scharenberg, Kyle Jacoby, Alexandra E. Grier
  • Long poly(A) plasmids and methods for introduction of long poly(A) sequences into the plasmid
    Patent number: 9943612
    Abstract: Disclosed herein are polynucleotides having a plurality of thymine nucleotides and an endonuclease recognition site inserted therein, methods of engineering the polynucleotides having a plurality of thymine nucleotides and an endonuclease recognition site inserted therein, and methods of enhancing transcription, translation, and increasing stability of a polynucleotide.
    Type: Grant
    Filed: October 8, 2015
    Date of Patent: April 17, 2018
    Assignee: Seattle Children's Hospital
    Inventors: Andrew M. Scharenberg, Kyle Jacoby, Alexandra E. Grier
  • LONG POLY(A) PLASMIDS AND METHODS FOR INTRODUCTION OF LONG POLY(A) SEQUENCES INTO THE PLASMID
    Publication number: 20160243258
    Abstract: Disclosed herein are polynucleotides having a plurality of thymine nucleotides and an endonuclease recognition site inserted therein, methods of engineering the polynucleotides having a plurality of thymine nucleotides and an endonuclease recognition site inserted therein, and methods of enhancing transcription, translation, and increasing stability of a polynucleotide.
    Type: Application
    Filed: October 8, 2015
    Publication date: August 25, 2016
    Inventors: Andrew M. Scharenberg, Kyle Jacoby, Alexandra E. Grier