Abstract: Disclosed herein are dielectrophoresis (DEP) devices having an alignment region, a DEP region, and an analysis region for characterizing particles. Disclosed herein are methods of characterizing particles, the methods include aligning particles along one or more walls of a microfluidic device, applying a non-uniform electric field to the particles, receiving the particles in a plurality of outlet channels, respectively, based on one or more electrical properties of the plurality of particles, passing at least one of the particles in one of the plurality of outlet channels through an impedance detector, and/or detecting an impedance measurement characteristic of at least one property of the at least one of the particles.

Abstract: Disclosed herein are microfluidic devices and methods of making microfluidic devices formed with low melting point metallic electrodes. Disclosed methods include adding a melted electrode material to an electrode channel of a microfluidic device and cooling the melted electrode to form an electrode.