Abstract: The invention provides a method for labeling a nucleic acid comprising N6-methyl adenine. The method comprises forming an alpha-amino radical on the N6-methyl group of N6mAde, and capturing the alpha-amino radical with a radical acceptor comprising a nitrosopyridyl group. The presence of N6mAde in a nucleic acid may then be established by detection of the labeled nucleic acid, or the labeled nucleic acid may be extracted or further modified using the label. Also provided is a method for mapping the position of N6mAde within a target nucleic acid, and probe molecules and kits for use in the method.

Abstract: Disclosed herein are aqueous compositions that include a pyridine complex and a polynucleotide. The compositions can be used to detect methylcytosine and/or hydroxymethylcytosine in the polynucleotide. In some examples, the compositions may be used as part of a TET-assisted borane sequencing workflow.

Abstract: Disclosed herein are methods of using photoredox reactions to modify 5-methylcytosine (5-mC), 5-hydroxymethylcytosine (5-hmC), 5-formlcytosine (5-fC), or 5-carboxylcytosine (5-caC) in a polynucleotide. In some examples, a photoredox reaction is used to install a functional group at the 5-position of the 5-caC, wherein the installed functional group further reacts with the 5-caC to form a product having at least two rings. In other examples, a photoredox reaction is used to install a functional group at the 5-methyl group of the 5-mC, wherein the installed functional group further reacts with the 5-mC to form a product having at least two rings. In other examples, a photoredox reaction is used to oxidize the 5-mC or 5-hmC to 5-fC; and a functional group at the 5-position of the 5-fC, wherein the installed functional group further reacts with the 5-fC to form a product having at least two rings.

Abstract: Embodiments of the present disclosure relate to nucleotide and nucleoside molecules with 3?-O-glycoside blocking groups. Also provided herein are methods to prepare such nucleotide and nucleoside molecules, and methods and kits for sequencing applications.

Abstract: The invention provides a method for labeling a nucleic acid comprising N6-methyl adenine. The method comprises forming an alpha-amino radical on the N6-methyl group of N6mAde, and capturing the alpha-amino radical with a radical acceptor comprising a nitrosopyridyl group. The presence of N6mAde in a nucleic acid may then be established by detection of the labeled nucleic acid, or the labeled nucleic acid may be extracted or further modified using the label. Also provided is a method for mapping the position of N6mAde within a target nucleic acid, and probe molecules and kits for use in the method.