Patents by Inventor Alexandre Lebedev

Alexandre Lebedev has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20220289786
    Abstract: Provided herein are methods and compositions for synthesizing 5?Capped RNAs wherein the initiating capped oligonucleotide primers have the general form m7Gppp[N2?Ome]n[N]m wherein m7G is N7-methylated guanosine or any guanosine analog, N is any natural, modified or unnatural nucleoside, “n” can be any integer from 0 to 4 and “m” can be an integer from 1 to 9.
    Type: Application
    Filed: May 26, 2022
    Publication date: September 15, 2022
    Applicant: TriLink Biotechnologies, LLC
    Inventors: Richard I. HOGREFE, Alexandre LEBEDEV, Anton P. MCCAFFREY, Dongwon SHIN
  • Patent number: 11414453
    Abstract: Provided herein are methods and compositions for synthesizing 5?Capped RNAs wherein the initiating capped oligonucleotide primers have the general form m7Gppp[N2?Ome]n[N]m wherein m7G is N7-methylated guanosine or any guanosine analog, N is any natural, modified or unnatural nucleoside, “n” can be any integer from 0 to 4 and “m” can be an integer from 1 to 9.
    Type: Grant
    Filed: August 2, 2021
    Date of Patent: August 16, 2022
    Assignee: TriLink Biotechnologies, LLC
    Inventors: Richard I. Hogrefe, Alexandre Lebedev, Anton P. McCaffrey, Dongwon Shin
  • Publication number: 20210395729
    Abstract: The present invention includes compositions and methods for the treatment of a medical condition or disease utilizing editing oligonucleotides. The editing oligonucleotides contain a an oligonucleotide strand of about 10 to about 50 nucleotides on each side of the editing moiety which may contain a sugar or linker that positions the active editing moiety in the proper location for hybridization to the target nucleic acid. The editing oligonucleotides may also contain at least one nucleotide sequence change from the targeted sequence in the genome. The method includes modifying a genomic sequence within a cell utilizing an editing oligonucleotide without additional proteins or nucleic acids to assist in the editing process. The editing oligonucleotide may comprise backbone modifications that increase the nuclease stability of the oligonucleotide as compared to unmodified oligonucleotides or oligonucleotides having three phosphorothioates on each terminus.
    Type: Application
    Filed: December 11, 2015
    Publication date: December 23, 2021
    Inventors: Tod M. WOOLF, Alexandre LEBEDEV, Richard I. HOGREFE
  • Publication number: 20210371452
    Abstract: Provided herein are methods and compositions for synthesizing 5?Capped RNAs wherein the initiating capped oligonucleotide primers have the general form m7Gppp[N2?Ome]n[N]m wherein m7G is N7-methylated guanosine or any guanosine analog, N is any natural, modified or unnatural nucleoside, “n” can be any integer from 0 to 4 and “m” can be an integer from 1 to 9.
    Type: Application
    Filed: August 2, 2021
    Publication date: December 2, 2021
    Applicant: TriLink Biotechnologies, LLC
    Inventors: Richard I. HOGREFE, Alexandre LEBEDEV, Anton P. MCCAFFREY, Dongwon SHIN
  • Publication number: 20210261597
    Abstract: Provided herein are methods and compositions for synthesizing 5?Capped RNAs wherein the initiating capped oligonucleotide primers have the general form m7 Gppp[N2?Ome]n[N]m wherein m7G is N7-methylated guanosine or any guanosine analog, N is any natural, modified or unnatural nucleoside, “n” can be any integer from 0 to 4 and “m” can be an integer from 1 to 9.
    Type: Application
    Filed: November 6, 2020
    Publication date: August 26, 2021
    Applicant: TriLink Biotechnologies, LLC
    Inventors: Richard I. HOGREFE, Alexandre LEBEDEV, Anton P. MCCAFFREY, Dongwon SHIN
  • Patent number: 10913768
    Abstract: Provided herein are methods and compositions for synthesizing 5?Capped RNAs wherein the initiating capped oligonucleotide primers have the general form m7Gppp[N2?Ome]n[N]m wherein m7G is N7-methylated guanosine or any guanosine analog, N is any natural, modified or unnatural nucleoside, “n” can be any integer from 0 to 4 and “m” can be an integer from 1 to 9.
    Type: Grant
    Filed: September 20, 2016
    Date of Patent: February 9, 2021
    Assignee: TRILINK BIOTECHNOLOGIES, INC.
    Inventors: Richard I. Hogrefe, Alexandre Lebedev, Anton P. McCaffrey, Dongwon Shin
  • Patent number: 10519189
    Abstract: Provided herein are methods and compositions for synthesizing 5?Capped RNAs wherein the initiating capped oligonucleotide primers have the general form m7Gppp[N2?Ome]n[N]m wherein m7G is N7-methylated guanosine or any guanosine analog, N is any natural, modified or unnatural nucleoside, “n” can be any integer from 0 to 4 and “m” can be an integer from 1 to 9.
    Type: Grant
    Filed: December 26, 2018
    Date of Patent: December 31, 2019
    Assignee: TRILINK BIOTECHNOLOGIES, INC.
    Inventors: Richard I. Hogrefe, Alexandre Lebedev, Anton P. McCaffrey, Dongwon Shin
  • Patent number: 10494399
    Abstract: Provided herein are methods and compositions for synthesizing 5?Capped RNAs wherein the initiating capped oligonucleotide primers have the general form m7Gppp[N2?Ome]n[N]m wherein m7G is N7-methylated guanosine or any guanosine analog, N is any natural, modified or unnatural nucleoside, “n” can be any integer from 0 to 4 and “m” can be an integer from 1 to 9.
    Type: Grant
    Filed: April 1, 2019
    Date of Patent: December 3, 2019
    Assignee: TRILINK BIOTECHNOLOGIES, INC.
    Inventors: Richard I. Hogrefe, Alexandre Lebedev, Anton P. McCaffrey, Dongwon Shin
  • Publication number: 20190270766
    Abstract: Provided herein are methods and compositions for synthesizing 5?Capped RNAs wherein the initiating capped oligonucleotide primers have the general form m7Gppp[N2?Ome]n[N]m wherein m7G is N7-methylated guanosine or any guanosine analog, N is any natural, modified or unnatural nucleoside, “n” can be any integer from 0 to 4 and “m” can be an integer from 1 to 9.
    Type: Application
    Filed: April 1, 2019
    Publication date: September 5, 2019
    Applicant: TRILINK BIOTECHNOLOGIES, INC.
    Inventors: Richard I. HOGREFE, Alexandre LEBEDEV, Anton P. MCCAFFREY, Dongwon SHIN
  • Publication number: 20190144490
    Abstract: Provided herein are methods and compositions for synthesizing 5?Capped RNAs wherein the initiating capped oligonucleotide primers have the general form m7Gppp[N2?Ome]n[N]m wherein m7G is N7-methylated guanosine or any guanosine analog, N is any natural, modified or unnatural nucleoside, “n” can be any integer from 0 to 4 and “m” can be an integer from 1 to 9.
    Type: Application
    Filed: December 26, 2018
    Publication date: May 16, 2019
    Applicant: TRILINK BIOTECHNOLOGIES, INC.
    Inventors: Richard I. Hogrefe, Alexandre Lebedev, Anton P. McCaffrey, Dongwon Shin
  • Publication number: 20180273576
    Abstract: Provided herein are methods and compositions for synthesizing 5?Capped RNAs wherein the initiating capped oligonucleotide primers have the general form m7Gppp[N2?Ome]n[N]m wherein m7G is N7-methylated guanosine or any guanosine analog, N is any natural, modified or unnatural nucleoside, “n” can be any integer from 0 to 4 and “m” can be an integer from 1 to 9.
    Type: Application
    Filed: September 20, 2016
    Publication date: September 27, 2018
    Applicant: TRILINK BIOTECHNOLOGIES, INC.
    Inventors: Richard I. HOGREFE, Alexandre LEBEDEV, Anton P. MCCAFFREY, Dongwon SHIN
  • Publication number: 20170275672
    Abstract: Provided herein are methods for ligation of polynucleotides containing modified ligation components, particularly modified ligase cofactors, modified acceptors and modified donors. The methods readily applied to ligation-based assays for detection of a nucleic acid sequence where the use of the modified cofactor improves discrimination between matched and mismatched templates. Furthermore, the use of the modified ligation components reduces or eliminates the ligation in the absence of nucleic acid template. In addition, methods are applied to the preparation of nucleic acid libraries using modified acceptor probes and modified donor probes that reduce or eliminate probe dimerization during the ligation process.
    Type: Application
    Filed: April 24, 2017
    Publication date: September 28, 2017
    Applicant: TriLink BioTechnologies, Inc.
    Inventors: Natasha Paul, Sabrina Shore, Jonathan Shum, Alexandre Lebedev, Gerald Zon
  • Patent number: 9631227
    Abstract: Provided herein are methods for ligation of polynucleotides containing modified ligation components, particularly modified ligase cofactors, modified acceptors and modified donors. The methods readily applied to ligation-based assays for detection of a nucleic acid sequence where the use of the modified cofactor improves discrimination between matched and mismatched templates. Furthermore, the use of the modified ligation components reduces or eliminates the ligation in the absence of nucleic acid template. In addition, methods are applied to the preparation of nucleic acid libraries using modified acceptor probes and modified donor probes that reduce or eliminate probe dimerization during the ligation process.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: April 25, 2017
    Assignee: TRILINK BIOTECHNOLOGIES, INC.
    Inventors: Natasha Paul, Sabrina Shore, Jonathan Shum, Alexandre Lebedev, Gerald Zon
  • Publication number: 20140323354
    Abstract: Provided herein are methods for ligation of polynucleotides containing modified ligation components, particularly modified ligase cofactors, modified acceptors and modified donors. The methods readily applied to ligation-based assays for detection of a nucleic acid sequence where the use of the modified cofactor improves discrimination between matched and mismatched templates. Furthermore, the use of the modified ligation components reduces or eliminates the ligation in the absence of nucleic acid template. In addition, methods are applied to the preparation of nucleic acid libraries using modified acceptor probes and modified donor probes that reduce or eliminate probe dimerization during the ligation process.
    Type: Application
    Filed: March 15, 2013
    Publication date: October 30, 2014
    Applicant: Trilink BioTechnologies, Inc.
    Inventors: Natasha Paul, Sabrina Shore, Jonathan Shum, Alexandre Lebedev, Gerald Zon
  • Patent number: 8728725
    Abstract: Provided herein are methods for ligation of polynucleotides containing modified ligation components, particularly modified ligase cofactors, modified acceptors and modified donors. The methods readily applied to ligation-based assays for detection of a nucleic acid sequence where the use of the modified cofactor improves discrimination between matched and mismatched templates. Furthermore, the use of the modified ligation components reduces or eliminates the ligation in the absence of nucleic acid template.
    Type: Grant
    Filed: July 6, 2010
    Date of Patent: May 20, 2014
    Assignee: Trilink Biotechnologies
    Inventors: Natasha Paul, Jonathan Shum, Alexandre Lebedev, Gerald Zon
  • Publication number: 20140038181
    Abstract: Provided herein are methods for ligase mediated nucleic acid replication and amplification of oligo- and probes containing substituted ligase components, particularly substituted ligase cofactors, substituted oligo- and probe acceptors, substituted oligo- and probe donors, substituted adenylated oligo- and polynucleotide donor intermediates carrying thermolabile group or groups. The substituted ligase components are not active until Hot Start activation step converts them into unsubstituted or natural ligase components, which fully support ligase reaction. The described methods are readily applied to ligation-based assays, especially utilizing Ligase Chain Reaction (LCR), for detection of a nucleic acid sequence where the use of the substituted ligase components improves an overall efficiency of LCR, increase discrimination between matched and mismatched templates and reduces or eliminates appearance of false positive signal.
    Type: Application
    Filed: July 3, 2013
    Publication date: February 6, 2014
    Inventors: Alexandre LEBEDEV, Inna KOUKHAREVA
  • Patent number: 8361753
    Abstract: The present invention provides methods and compositions for nucleic acid amplification. These methods involve the use of oligonucleotide primers in temperature dependent nucleic acid amplification reactions. In certain aspects, the methods are accomplished by use of certain modified oligonucleotide primers which provide utility in nucleic acid amplification. In preferred embodiments, the oligonucleotide primers are modified with particular chemical groups such as esters.
    Type: Grant
    Filed: May 17, 2007
    Date of Patent: January 29, 2013
    Assignee: TriLink BioTechnologies, Inc.
    Inventors: Gerald Zon, Alexandre Lebedev
  • Patent number: 8133669
    Abstract: Provided herein are methods and compositions for nucleic acid replication. These methods involve the use of 3?-substituted nucleoside 5?-triphosphates or 3?-substituted terminated primers in nucleic acid replication reactions. In certain aspects, the methods are accomplished by use of 3?-substituted NTPs and/or 3?-substituted terminated primers which provide utility in nucleic acid replication. In preferred embodiments, the NTPs and/or primers are substituted at the 3?-position with particular heat labile chemical groups such as ethers, esters or carbonate esters.
    Type: Grant
    Filed: May 21, 2009
    Date of Patent: March 13, 2012
    Assignee: Trilink Biotechnologies
    Inventors: Alexandre Lebedev, Inna Koukhareva
  • Publication number: 20110008788
    Abstract: Provided herein are methods for ligation of polynucleotides containing modified ligation components, particularly modified ligase cofactors, modified acceptors and modified donors. The methods readily applied to ligation-based assays for detection of a nucleic acid sequence where the use of the modified cofactor improves discrimination between matched and mismatched templates. Furthermore, the use of the modified ligation components reduces or eliminates the ligation in the absence of nucleic acid template.
    Type: Application
    Filed: July 6, 2010
    Publication date: January 13, 2011
    Inventors: Natasha Paul, Jonathan Shum, Alexandre Lebedev, Gerald Zon
  • Publication number: 20100003724
    Abstract: Provided herein are methods and compositions for nucleic acid replication. These methods involve the use of 3?-substituted nucleoside 5?-triphosphates or 3?-substituted terminated primers in nucleic acid replication reactions. In certain aspects, the methods are accomplished by use of 3?-substituted NTPs and/or 3?-substituted terminated primers which provide utility in nucleic acid replication. In preferred embodiments, the NTPs and/or primers are substituted at the 3?-position with particular heat labile chemical groups such as ethers, esters or carbonate esters.
    Type: Application
    Filed: May 21, 2009
    Publication date: January 7, 2010
    Inventors: Alexandre Lebedev, Inna Koukhareva