Abstract: A process for the biological purification of effluent which is contaminated with nitrogen in the form of ammonium, nitrite or nitrate ions or in the form of organic compounds, by oxidizing the nitrogen under aerobic conditions and reducing the nitrogen under anaerobic conditions, wherein the sewage to be treated, mixed with activated sludge, is subjected, in an aeration basin, to oxygen-containing gas bubbles introduced from below and is thereby simultaneously enriched with dissolved oxygen, to oxidize carbon compounds and nitrogen compounds, and transported upward, by the buoyancy of the gas bubbles, releases gas at the surface of the liquid and, due to the higher average density of the mixture of lower gas content, in part automatically again flows downward in the aeration basin, to undergo further aeration, while in part it overflows into a denitrification basin which communicates with the aeration basin through an opening at the liquid surface, and an opening at the bottom of the partition, is mixed, in t