Abstract: The invention relates to a device (1) for creating a patterned evanescent field on the surface (S) of a dioptre comprising an objective lens (O), in an image focal plane of which the surface (S) of the dioptre is arranged, a light injection element (2) emitting a collimated light beam (B), an optical assembly (4) between the element (2) and the objective lens (O) by which the object plane of the objective lens (O) is optically conjugate with the image plane of the element (2), the assembly (4) being configured so that a collimated light beam (B) from the element (4) is emitted towards the objective lens (O) to be reflected towards the surface (S) of the dioptre with an angle of incidence greater than or equal to the critical angle of the dioptre, an optical device (FS1) for forming patterns is in the object plane of the element (2), so that the pattern formed by the optical device for forming patterns in transmitted light on the light beam in the object plane of the element (2) is found on the surface of the

Abstract: A disclosed method involves: receiving, by a processing device, a first plurality of photon count values indicating a number of photons detected by an optical system during a plurality of first time periods as a result of laser beam excitation of an observation volume of a sample during fluorescence fluctuation microscopy analysis; calculating, by the processing device based on the first plurality of photon count values, a first count rate per molecule indicating the average number of photons detected per molecule of the observation volume; and generating, by the processing device based on the first count rate per molecule, a control signal for configuring a phase modulation device of the optical system.

Abstract: A disclosed method involves: receiving, by a processing device, a first plurality of photon count values indicating a number of photons detected by an optical system during a plurality of first time periods as a result of laser beam excitation of an observation volume of a sample during fluorescence fluctuation microscopy analysis; calculating, by the processing device based on the first plurality of photon count values, a first count rate per molecule indicating the average number of photons detected per molecule of the observation volume; and generating, by the processing device based on the first count rate per molecule, a control signal for configuring a phase modulation device of the optical system.