Abstract: System, apparatus and method of testing a sample capable of transporting oxygen and including a dye, wherein the system includes: an illumination device to generate a first light pulse for a first period and second light pulses for each second period, the first pulse and second pulses illuminating the sample; a first detector to collect absorbance spectrum measurements from the sample illuminated by the first pulse; a second detector to collect phosphorescence measurements emitted by the dye of the sample illuminated by the second pulses, each phosphorescence measurement collected during a third period after a corresponding second pulse; a microcontroller to generate an absorbance spectrum average of the spectrum measurements, and to generate a phosphorescence average of phosphorescence measurements; and a computing device to calculate SatHb based on the spectrum average and calculate PO2 based on the phosphorescence average, and plot a point SatHb, PO2 on an oxygen equilibrium curve.

Abstract: A method of distinguishing between proteinaceous and non-proteinaceous particulates in a fluid sample includes the steps of acquiring a brightfield background image of a membrane filter, introducing a fluid sample onto the membrane filter, acquiring a brightfield image of filtered particles resting on the membrane filter, generating a particle mask based on the brightfield background image and the brightfield image of filtered particles, introducing a fluorescent dye onto the membrane filter, detecting fluorescence on the particle mask, and distinguishing between proteinaceous and non-proteinaceous particulates based on the detected fluorescence. A method for detecting other types of particles, such as polysorbate particles, silicone oil or protein monomers is also disclosed.

Abstract: A method of distinguishing between proteinaceous and non-proteinaceous particulates in a fluid sample includes the steps of acquiring a brightfield background image of a membrane filter, introducing a fluid sample onto the membrane filter, acquiring a brightfield image of filtered particles resting on the membrane filter, generating a particle mask based on the brightfield background image and the brightfield image of filtered particles, introducing a fluorescent dye onto the membrane filter, detecting fluorescence on the particle mask, and distinguishing between proteinaceous and non-proteinaceous particulates based on the detected fluorescence. A method for detecting other types of particles, such as polysorbate particles, silicone oil or protein monomers is also disclosed.

Abstract: A method of distinguishing between proteinaceous and non-proteinaceous particulates in a fluid sample includes the steps of acquiring a brightfield background image of a membrane filter, introducing a fluid sample onto the membrane filter, acquiring a brightfield image of filtered particles resting on the membrane filter, generating a particle mask based on the brightfield background image and the brightfield image of filtered particles, introducing a fluorescent dye onto the membrane filter, detecting fluorescence on the particle mask, and distinguishing between proteinaceous and non-proteinaceous particulates based on the detected fluorescence. A method for detecting other types of particles, such as polysorbate particles, silicone oil or protein monomers is also disclosed.

Abstract: The present invention includes a device useful for evaluating the size, shape, surface texture, mobility, rigidity, flexibility, tensile resistance or turnover rate of red blood cells in a blood sample from an individual. The device of the present invention may be used to determine if the individual suffers from a red blood cell abnormality, such as sickle cell disease.