Abstract: The present invention relates to a reaction unit (10) configured to receive a reaction solution or culture media and configured to be placed inside a thermocycler, said reaction unit (10) comprising an elongated hollow body (12) extending along a flowing axis X, the hollow body (12) thus displaying a first opening (14) at its first extremity (16) and a second opening (18) at its second extremity (20). The walls of the hollow body (12) are at least partially made of a thermally conductive material. The reaction unit (10) further comprises at least one filter element (22) extending inside the hollow body (12), the filter element (22) being sealingly secured to the walls of the hollow body (12) over its complete circumference, leading any fluid flowing from the first opening (14) to the second opening (18) to cross the at least one filter element (22).

Abstract: Thermocycling multistep reactions device (10) comprising: a reaction plate (12) comprising at least one well (14), at least one internal thermoregulating fluid circulation circuit configured to be arranged in direct contact with the at least one well (14), an evacuation system, a control unit configured to manage the temperature of the internal thermoregulating fluid circuit. The temperature inside the at least one well (14) is regulated and controlled by means of the control unit. Each well (14) is connected to the evacuation system by means of at least one filter membrane, and the thermoregulating fluid circuit is made of a heat conducting material while the reaction plate is made (12) of a polymerous material.