Patents by Inventor Alfons Nichtl
Alfons Nichtl has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220317119Abstract: Disclosed is a method for measurement of an analyte in a microparticle-based analyte-specific binding assay, wherein the microparticles are coated with the first partner of a binding pair, the method involving mixing the coated microparticles, an analyte-specific binding agent conjugated to the second partner of the binding pair, and a sample suspected of containing or containing the analyte, wherein the second partner of the binding pair is bound to the analyte-specific binding agent via a linker having from 12 to 30 ethylene glycol units (PEG 12 to 30), thereby binding the analyte via the conjugated analyte-specific binding agent to the coated microparticles, separating the microparticles having the analyte bound via the binding pair and the analyte-specific binding agent from the mixture and measuring the analyte bound to the microparticles.Type: ApplicationFiled: June 13, 2022Publication date: October 6, 2022Inventors: Toralf ZARNT, Dieter HEINDL, Alfons NICHTL, Frank SICHERL-BIRK
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Patent number: 11391733Abstract: Disclosed is a method for measurement of an analyte in a microparticle-based analyte-specific binding assay, wherein the microparticles are coated with the first partner of a binding pair, the method involving mixing the coated microparticles, an analyte-specific binding agent conjugated to the second partner of the binding pair, and a sample suspected of containing or containing the analyte, wherein the second partner of the binding pair is bound to the analyte-specific binding agent via a linker having from 12 to 30 ethylene glycol units (PEG 12 to 30), thereby binding the analyte via the conjugated analyte-specific binding agent to the coated microparticles, separating the microparticles having the analyte bound via the binding pair and the analyte-specific binding agent from the mixture and measuring the analyte bound to the microparticles.Type: GrantFiled: February 19, 2018Date of Patent: July 19, 2022Assignee: Roche Diagnostics Operations, Inc.Inventors: Toralf Zarnt, Dieter Heindl, Alfons Nichtl, Frank Sicherl-Birk
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Recombinant immunoglobulin heavy chains comprising a sortase conjugation loop and conjugates thereof
Patent number: 11136376Abstract: The disclosure describes a recombinant immunoglobulin heavy chain comprising a sortase conjugation loop, methods for conjugating and/or labeling such recombinant immunoglobulin heavy chains by use of the enzyme sortase and to the conjugates/labeled products obtained via the method.Type: GrantFiled: March 23, 2018Date of Patent: October 5, 2021Assignee: Roche Diagnostics Operations, Inc.Inventors: Alfred Engel, Pavel Kubalec, Alfons Nichtl, Tobias Oelschlaegel, Rainer Schlecht -
RECOMBINANT IMMUNOGLOBULIN HEAVY CHAINS COMPRISING A SORTASE CONJUGATION LOOP AND CONJUGATES THEREOF
Publication number: 20210009660Abstract: The disclosure describes a recombinant immunoglobulin heavy chain comprising a sortase conjugation loop, methods for conjugating and/or labeling such recombinant immunoglobulin heavy chains by use of the enzyme sortase and to the conjugates/labeled products obtained via the method.Type: ApplicationFiled: March 23, 2018Publication date: January 14, 2021Applicant: Roche Diagnostics Operations, Inc.Inventors: Alfred Engel, Pavel Kubalec, Alfons Nichtl, Tobias Oelschlaegel, Rainer Schlecht -
Publication number: 20180172680Abstract: Disclosed is a method for measurement of an analyte in a microparticle-based analyte-specific binding assay, wherein the microparticles are coated with the first partner of a binding pair, the method involving mixing the coated microparticles, an analyte-specific binding agent conjugated to the second partner of the binding pair, and a sample suspected of containing or containing the analyte, wherein the second partner of the binding pair is bound to the analyte-specific binding agent via a linker having from 12 to 30 ethylene glycol units (PEG 12 to 30), thereby binding the analyte via the conjugated analyte-specific binding agent to the coated microparticles, separating the microparticles having the analyte bound via the binding pair and the analyte-specific binding agent from the mixture and measuring the analyte bound to the microparticles.Type: ApplicationFiled: February 19, 2018Publication date: June 21, 2018Applicant: Roche Diagnostics Operations, Inc.Inventors: Toralf Zarnt, Dieter Heindl, Alfons Nichtl, Frank Sicherl-Birk
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Patent number: 9140709Abstract: The present invention concerns an immunological test for determining NT-proBNP comprising at least two antibodies to NT-proBNP, wherein at least one of the antibodies to NT-proBNP is a monoclonal antibody. The epitopes recognized by the antibodies can slightly overlap.Type: GrantFiled: January 15, 2009Date of Patent: September 22, 2015Assignee: Roche Diagnostics Operations, Inc.Inventors: Juergen Spinke, Alfons Nichtl, Volker Klemt, Klaus Hallermayer, Michael Grol, Anneliese Borgya, Andreas Gallusser
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Patent number: 8981070Abstract: An oligonucleotide-solid phase conjugate, wherein the solid phase is thiophilic and the oligonucleotide comprises at least one thiooxonucleobase according to Formula I, wherein X is CH or N, R1 is H or NH2, --- indicates a covalent bond, and said oligonucleotide is bound to said solid phase via the sulfur atom of said thiooxonucleotide. Also disclosed are methods for producing such conjugate as well as various uses for such oligonucleotide metal conjugate.Type: GrantFiled: April 19, 2013Date of Patent: March 17, 2015Assignee: Roche Diagnostics Operations, Inc.Inventors: Simone Budow, Ping Ding, Dieter Heindl, Alfons Nichtl, Frank Seela
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Publication number: 20130210009Abstract: An oligonucleotide-solid phase conjugate, wherein the solid phase is thiophilic and the oligonucleotide comprises at least one thiooxonucleobase according to Formula I, wherein X is CH or N, R1 is H or NH2, --- indicates a covalent bond, and said oligonucleotide is bound to said solid phase via the sulfur atom of said thiooxonucleotide. Also disclosed are methods for producing such conjugate as well as various uses for such oligonucleotide metal conjugate.Type: ApplicationFiled: April 19, 2013Publication date: August 15, 2013Applicant: ROCHE DIAGNOSTICS OPERATIONS, INC.Inventors: Simone Budow, Ping Ding, Dieter Heindl, Alfons Nichtl, Frank Seela
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Publication number: 20100291696Abstract: A process for detecting nucleic acids, having the following steps: providing at least one nanoparticle that is functionalised for the nucleic acid to be detected by means of at least one oligonucleotide that is bound to it and that is able to hybridize with at least one segment of a nucleic acid to be detected; bringing the functionalised nanoparticle into contact with a sample in which the nucleic acid is to be detected; and measuring a property that provides information about the degree of hybridization of the at least one oligonucleotide with the nucleic acid to be detected. In addition, the process includes the step of exciting the nanoparticles to generate heat, for example by means of a photothermal effect. The invention is suitable, in particular, for high-throughput DNA analysis.Type: ApplicationFiled: May 27, 2008Publication date: November 18, 2010Applicant: LUDWIG-MAXIMILIANS-UNIVERSITÄT MÜNCHENInventors: Joachim A. Stehr, Thomas A. Klar, Jochen Feldmann, Calin Hrelescu, Wolfgang Parak, Gunnar Raschke, Ralf Sperling, Michael Wunderlich, Konrad Kürzinger, Dieter Heindl, Alfons Nichtl
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Patent number: 7662920Abstract: The present invention relates to a method of producing a fluorescent polypeptide complex characterized in that an isolated fluorescent polypeptide is inter-molecularly cross-linked with itself or one or more other polypeptides and wherein said complex is of 40 nm to 500 nm in size, it also relates to a complex obtainable by such method, to conjugates comprising such a fluorescent polypeptide complex and to the use of such complex or conjugate.Type: GrantFiled: October 5, 2006Date of Patent: February 16, 2010Assignee: Roche Diagnostics Operations, Inc.Inventors: Albert Geiger, Hans Hornauer, Alfons Nichtl, Peter Sluka
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Publication number: 20090123947Abstract: The present invention concerns an immunological test for determining NT-proBNP comprising at least two antibodies to NT-proBNP, wherein at least one of the antibodies to NT-proBNP is a monoclonal antibody. The epitopes recognized by the antibodies can slightly overlap.Type: ApplicationFiled: January 15, 2009Publication date: May 14, 2009Inventors: Juergen Spinke, Alfons Nichtl, Volker Klemt, Klaus Hallermayer, Michael Grol, Anneliese Borgya, Andreas Gallusser
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Patent number: 7507550Abstract: The present invention concerns an immunological test for determining NT-proBNP comprising at least two antibodies to NT-proBNP, wherein at least one of the antibodies to NT-proBNP is a monoclonal antibody. One of these antibodies is directed at least against parts of the epitope of NT-proBNP comprising the amino acids 38 to 50. In addition, one of these antibodies is directed at least against parts of the epitope of NT-proBNP comprising the amino acids 1 to 37 or 43 to 76. The epitope recognized by the antibodies can slightly overlap.Type: GrantFiled: November 22, 2004Date of Patent: March 24, 2009Assignee: Roche Diagnostics Operations, Inc.Inventors: Juergen Spinke, Alfons Nichtl, Volker Klemt, Klaus Hallermayer, Michael Grol, Anneliese Borgya, Andreas Gallusser
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Patent number: 7118875Abstract: The invention concerns an analytical element for the determination of an analyte containing in or on material which enables liquid transport between zones, a sample application zone and a detection zone located downstream thereof, wherein the detection zone contains a partner 1 of a specific binding pair 1 immobilized in such a manner that it is able to bind to partner 2 of the specific binding pair 1 which is not the analyte when it contacts it, wherein a labelled partner 1 of a specific binding pair 2 is present upstream of the detection zone impregnated on a material such that it can be detached by liquid and is able to bind to partner 2 of the specific binding pair 2 which is not the analyte when this contacts it as well as a method for the determination of an analyte using this analytical element.Type: GrantFiled: December 30, 2003Date of Patent: October 10, 2006Assignee: Roche Diagnostics GmbHInventors: Jürgen Klepp, Gerhard Hiller, Alfons Nichtl, Thomas Fischer, Martina Hösch
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Publication number: 20050118662Abstract: The present invention concerns an immunological test for determining NT-proBNP comprising at least two antibodies to NT-proBNP, wherein at least one of the antibodies to NT-proBNP is a monoclonal antibody. One of these antibodies is directed at least against parts of the epitope of NT-proBNP comprising the amino acids 38 to 50. In addition, one of these antibodies is directed at least against parts of the epitope of NT-proBNP comprising the amino acids 1 to 37 or 43 to 76. The epitope recognized by the antibodies can slightly overlap.Type: ApplicationFiled: November 22, 2004Publication date: June 2, 2005Inventors: Juergen Spinke, Alfons Nichtl, Volker Klemt, Klaus Hallermayer, Michael Grol, Anneliese Borgya, Andreas Gallusser
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Patent number: 6833275Abstract: A composition which contains stabilized conjugates composed of colloidal particles and biomolecules is obtained by adding a detergent to a solution containing biomolecules before or/and during treatment of colloidal particles with this solution.Type: GrantFiled: August 7, 2000Date of Patent: December 21, 2004Assignee: Roche Diagnostics GmbHInventor: Alfons Nichtl
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Publication number: 20040152142Abstract: The invention concerns an analytical element for the determination of an analyte containing in or on material which enables liquid transport between zones, a sample application zone and a detection zone located downstream thereof, wherein the detection zone contains a partner 1 of a specific binding pair 1 immobilized in such a manner that it is able to bind to partner 2 of the specific binding pair 1 which is not the analyte when it contacts it, wherein a labelled partner 1 of a specific binding pair 2 is present upstream of the detection zone impregnated on a material such that it can be detached by liquid and is able to bind to partner 2 of the specific binding pair 2 which is not the analyte when this contacts it as well as a method for the determination of an analyte using this analytical element.Type: ApplicationFiled: December 30, 2003Publication date: August 5, 2004Inventors: Jurgen Klepp, Gerhard Hiller, Alfons Nichtl, Thomas Fischer, Martina Hosch
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Patent number: 6703196Abstract: The invention concerns an analytical element for the determination of an analyte containing in or on material which enables liquid transport between zones, a sample application zone and a detection zone located downstream thereof, wherein the detection zone contains a partner 1 of a specific binding pair 1 immobilized in such a manner that it is able to bind to partner 2 of the specific binding pair 1 which is not the analyte when it contacts it, wherein a labelled partner 1 of a specific binding pair 2 is present upstream of the detection zone impregnated on a material such that it can be detached by liquid and is able to bind to partner 2 of the specific binding pair 2 which is not the analyte when this contacts it as well as a method for the determination of an analyte using this analytical element.Type: GrantFiled: December 18, 1998Date of Patent: March 9, 2004Assignee: Roche Diagnostics CorporationInventors: Jürgen Klepp, Gerhard Hiller, Alfons Nichtl, Ulrike Fischer, Martina Hösch
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Patent number: 5972720Abstract: The invention concerns a composition comprising colloidal particles to the surface of which biomolecules are adsorbed, wherein the composition additionally contains a polyethylene glycol substituted by thiol or/and disulfide groups. This composition can be used as a detection reagent in immunological test methods.Type: GrantFiled: June 5, 1997Date of Patent: October 26, 1999Assignee: Roche Diagnostics GmbHInventors: Alfons Nichtl, Peter Sluka