Abstract: The present invention is directed to a recirculation system for use in microfluidic centrifugal disc platforms for reusing and mixing an entire sample. The present invention features a system comprising a reservoir, an input channel, a detection array, a pressure chamber, and a recirculation channel connecting the pressure chamber to the reservoir. The recirculation channel may have a resistance lower than the channel upstream resistance. When the CD platform spins at a high RPM, the liquid may be directed from the reservoir into the pressure chamber. When the RPM of the CD platform decreases rapidly, the liquid may be from the pressure chamber through the channel and through the recirculation channel to the reservoir, such that the liquid travels through the recirculation channel faster than the liquid travels through the channel.

Abstract: The present invention is directed to an apparatus and method of fabricating a sealed storage system for environmentally sensitive aqueous materials with liquid flow control and on-demand distribution. The present invention features a sealed storage apparatus capable of compactly containing a liquid reagent with liquid control capabilities. The apparatus may comprise a liquid-impermeable, air-impermeable, resistant to force-induced tearing pouch body capable of being penetrated through the application of a laser. The apparatus may further comprise a pouch cavity disposed within the pouch body for holding the liquid reagent. The apparatus may further comprise a liquid-impermeable, air-impermeable, and resistant to force-induced tearing sealing adhesive disposed over the pouch cavity to seal the liquid reagent within the pouch body capable of being penetrated through the application of the laser.