Patents by Inventor Alfred M. Handler

Alfred M. Handler has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • System for gene targeting and producing stable genomic transgene insertions
    Patent number: 7700356
    Abstract: The novel germ-line transformation systems disclosed in this patent application allow the physical deletion of transposon DNA following the transformation process, and the targeting of transgene integrations into predefined target sites. In this way, transposase-mediated mobilization of genes-of-interest is excluded mechanistically and random genomic integrations eliminated. In contrast to conventional germ-line transformation technology, our systems provide enhanced stability to the transgene insertion. Furthermore, DNA sequences required for the transgene modification (e.g. transformation marker genes, transposase or recombinase target sites), are largely removed from the genome after the final transgene insertion, thereby eliminating the possibility for instability generated by these processes.
    Type: Grant
    Filed: November 7, 2003
    Date of Patent: April 20, 2010
    Assignee: The United States of America as represented by the Secretary of Agriculture
    Inventors: Carsten Horn, Alfred M. Handler
  • Systems for gene targeting and producing stable genomic transgene insertions
    Publication number: 20090083870
    Abstract: The novel germ-line transformation systems disclosed in this patent application allow the physical deletion of transposon DNA following the transformation process, and the targeting of transgene integrations into predefined target sites. In this way, transposase-mediated mobilization of genes-of-interest is excluded mechanistically and random genomic integrations eliminated. In contrast to conventional germ-line transformation technology, our systems provide enhanced stability to the transgene insertion. Furthermore, DNA sequences required for the transgene modification (e.g. transformation marker genes, transposase or recombinase target sites), are largely removed from the genome after the final transgene insertion, thereby eliminating the possibility for instability generated by these processes.
    Type: Application
    Filed: July 11, 2008
    Publication date: March 26, 2009
    Inventors: Carsten Horn, Alfred M. Handler
  • PiggyBac transformation system
    Patent number: 7129083
    Abstract: The present invention is directed to a transformation systems and vectors for making transgenic organisms that includes a vector containing a modified piggyBac transposon into which is inserted at least one fluorescent protein gene linked to a polyubiquitin promoter sequence. A helper transposase vector that includes an hsp70 promoter sequence upstream of the putative piggyBac promoter that increases the transformation frequency of this system can also be included.
    Type: Grant
    Filed: July 27, 2001
    Date of Patent: October 31, 2006
    Assignee: The United States of America as represented by the Secretary of Agriculture
    Inventor: Alfred M. Handler
  • PiggyBac transformation system
    Patent number: 7005296
    Abstract: The present invention is directed to a transformation system for making transgenic organisms that includes a vector containing a modified piggyBac transposon into which is inserted an enhanced green fluorescent protein gene linked to a polyubiquitin promoter sequence and a nuclear localizing sequence; and a helper transposase vector that includes an hsp7O promoter sequence upstream of the putative piggyBac promoter that increases the transformation frequency of this system.
    Type: Grant
    Filed: March 21, 2002
    Date of Patent: February 28, 2006
    Assignee: The United States of America as represented by the Secretary of Agriculture
    Inventor: Alfred M. Handler
  • PiggyBac transformation system
    Patent number: 6773914
    Abstract: The present invention is directed to a transformation system for making transgenic organisms that includes a vector containing a modified piggyBac transposon into which is inserted an enhanced green fluorescent protein gene linked to a polyubiquitin promoter sequence and a nuclear localizing sequence; and a helper transposase vector that includes an hsp70 promoter sequence upstream of the putative piggyBac promoter that increases the transformation frequency of this system.
    Type: Grant
    Filed: August 19, 1999
    Date of Patent: August 10, 2004
    Assignee: The United States of America as represented by the Secretary of Agriculture
    Inventor: Alfred M. Handler