Abstract: Contemporary gene sequencing techniques, including “Next Generation Sequencing” techniques, can include sequencing a plurality of fragments of a target polynucleotide. However, tire limitations of existing sequencing techniques, and the often repetitive or otherwise difficult-to-sequence structure of natural polynucleotides, means that it can be difficult and/or expensive to generate accurate sequences. Methods provided herein include inserting dual polynucleotide ‘barcodes,’ along with neighboring primer sequences, into a target polynucleotide prior to other sequencing processes. These inserted barcodes can improve the accuracy of sequences generated for the target by adding ‘noise’ into the target, allowing subsequent sequencing techniques (e.g., alignment, stitching, etc.) to more accurately estimate the target-plus-barcodes sequence.

Abstract: Contemporary gene sequencing techniques, including “Next. Generation Sequencing” techniques, can include sequencing a plurality of fragments of a target polynucleotide. However, the limitations of existing sequencing techniques means that it can be difficult and/or expensive to align the generated read fragments. Methods provided herein include inserting dual polynucleotide ‘bar-codes’ into a target poly nucleotide that remain mechanically connected via a Tinker.’ Tire barcodes can then be ‘grown’ via. a. pool-split-pool process such that polynucleotide fragments that are linked by linkers exhibit the same complete barcode sequence that is different, from the complete barcode sequence exhibited by non-linked polynucleotide fragments. The joined fragments can then be separated and sequenced.

Abstract: This disclosure describes methods and compositions for protein and peptide sequencing.

Abstract: A method is provided for determining a sample genome from a plurality of read fragments and a reference genome. The method includes: (i) applying a first putative variant event, selected from a set of candidate variant events, to the sample genome to update the sample genome; (ii) mapping the plurality of read fragments to the updated sample genome; (hi) based on the mapping of the plurality of read fragments to the updated sample genome, determining a first read mapping cost function; and (iv) based on the first read mapping cost function, retaining the updated sample genome and removing the first putative variant event from the set of candidate variant events.

Abstract: This disclosure describes methods and compositions for protein and peptide sequencing.

Abstract: Contemporary gene sequencing techniques, including “Next Generation Sequencing” techniques, can include sequencing a plurality of fragments of a target polynucleotide. These fragment sequences are then used to determine a sequence for the target as a whole. This can include aligning the fragment sequences to each other anchor to a reference genome. However, the limitations of existing sequencing techniques, and the often repetitive or otherwise difficult-to-sequence structure of natural polynucleotides, means that it can be difficult and/or expensive to generate accurate sequences. Methods provided herein include inserting polynucleotide ‘barcodes’ into a target polynucleotide prior to fragmentation or other sequencing processes. These inserted barcodes can improve the accuracy of sequences generated for the target by adding ‘noise’ into the target, allowing subsequent sequencing techniques (e.g., alignment, stitching, etc.) to more accurately estimate the target-plus-barcodes sequence.

Abstract: Contemporary gene sequencing techniques, including “Next Generation Sequencing” techniques, can include sequencing a plurality of fragments of a target polynucleotide. However, tire limitations of existing sequencing techniques, and the often repetitive or otherwise difficult-to-sequence structure of natural polynucleotides, means that it can be difficult and/or expensive to generate accurate sequences. Methods provided herein include inserting dual polynucleotide ‘barcodes,’ along with neighboring primer sequences, into a target polynucleotide prior to other sequencing processes. These inserted barcodes can improve the accuracy of sequences generated for the target by adding ‘noise’ into the target, allowing subsequent sequencing techniques (e.g., alignment, stitching, etc.) to more accurately estimate the target-plus-barcodes sequence.

Abstract: This disclosure describes methods and compositions for protein and peptide sequencing.

Abstract: This disclosure describes methods and compositions for protein and peptide sequencing.

Abstract: Methods, devices, and systems for performing intermittent detection during analytical reactions are provided. Such methods facilitate collection of reaction data from disparate reaction times. Further, such methods are useful for reducing photo-induced damage of one or more reactants in an illuminated analytical reaction at a given reaction time. In preferred embodiments, the reaction mixture is subjected to at least one illuminated and non-illuminated period and allowed to proceed such that the time in which the reaction mixture is illuminated is less than a photo-induced damage threshold period.

Abstract: Methods, devices, and systems for performing intermittent detection during analytical reactions are provided. Such methods facilitate collection of reaction data from disparate reaction times. Further, such methods are useful for reducing photo-induced damage of one or more reactants in an illuminated analytical reaction at a given reaction time. In preferred embodiments, the reaction mixture is subjected to at least one illuminated and non-illuminated period and allowed to proceed such that the time in which the reaction mixture is illuminated is less than a photo-induced damage threshold period.