Abstract: The invention is a nanowire-comprising device for detecting at least one target biomolecule. The nanowires are placed between a substrate and a multilayer structure. The multilayer structure lies between the nanowires and a sample liable to contain a target biomolecule. The multilayer structure comprises a functionalization surface making contact with the sample. When light is emitted from the functionalization surface, fluorescence light for example, at least one nanowire allows the light to be detected. The fluorescence light may indicate the presence or absence of a target biomolecule on the functionalization surface.