Abstract: In certain embodiments, the invention relates to systems and methods for in vivo tomographic imaging of fluorescent probes and/or bioluminescent reporters, wherein a fluorescent probe and a bioluminescent reporter are spatially co-localized (e.g., located at distances equivalent to or smaller than the scattering mean free path of light) in a diffusive medium (e.g., biological tissue). Measurements obtained from bioluminescent and fluorescent modalities are combined per methods described herein.

Abstract: An objective lens switching device includes a prismatic cuvette perpendicular to a plane parallel to a direction of detection and a lighting direction, which are mutually perpendicular. The cross-section of the prism is a polygon with more than four sides with pairs of mutually perpendicular faces. At least several pairs of faces include one face designed to receive a planar light beam in the lighting direction and one face with a coupled lens or objective lens to detect fluorescent light in the direction of detection. The cuvette can rotate about an axis perpendicular to said plane, allowing a specific objective lens to be oriented in the direction of detection.

Abstract: In certain embodiments, the invention relates to systems and methods for in vivo tomographic imaging of fluorescent probes and/or bioluminescent reporters, wherein a fluorescent probe and a bioluminescent reporter are spatially co-localized (e.g., located at distances equivalent to or smaller than the scattering mean free path of light) in a diffusive medium (e.g., biological tissue). Measurements obtained from bioluminescent and fluorescent modalities are combined per methods described herein.

Abstract: In certain embodiments, the invention relates to systems and methods for in vivo tomographic imaging of fluorescent probes and/or bioluminescent reporters, wherein a fluorescent probe and a bioluminescent reporter are spatially co-localized (e.g., located at distances equivalent to or smaller than the scattering mean free path of light) in a diffusive medium (e.g., biological tissue). Measurements obtained from bioluminescent and fluorescent modalities are combined per methods described herein.

Abstract: The invention describes an objective lens switching device (1) comprising a prismatic cuvette (2) perpendicular to a plane parallel to a direction of detection (DD) and a lighting direction (DI) which are mutually perpendicular. The cross-section of the prism is a polygon with more than four sides with pairs of mutually perpendicular faces (2a, 2a?; 2b, 2b?; 2c, 2c?; 2d, 2d). Furthermore, at least several pairs of faces (2a, 2a?; 2b, 2b?; 2c, 2e; 2d, 2d) comprise one face (2a?, 2b?, 2e, 2d?) designed to receive a planar light beam (6) in the lighting direction (DI) and one face (2a, 2b, 2c, 2c) with a coupled lens or objective lens (9a, 9b, 9c, 9d) to detect fluorescent light (8) in the direction of detection (DD). The cuvette (2) can rotate about an axis perpendicular to said plane, allowing a specific objective lens (9a, 9b, 9c, 9d) to be oriented in the direction of detection (DD).

Abstract: The invention relates to a microscope and a method for producing 3D images of various transparent or semi-transparent samples, fundamentally comprising: causing a relative movement according to the detection direction between the sheet of light and the sample while maintaining a constant angle of acquisition; producing, for said angle of acquisition, a single 2D projection image formed by a representative parameter for each pixel; modifying the angle of acquisition by means of a relative rotation between the sheet of light and the sample, combined with a relative vertical translation between the sheet of light and the sample, and repeating the previous steps; and generating a 3D image of each of the samples from the set of 2D projection images that are produced.

Abstract: The invention relates to a microscope and a method for producing 3D images of various transparent or semi-transparent samples, fundamentally comprising: causing a relative movement according to the detection direction between the sheet of light and the sample while maintaining a constant angle of acquisition; producing, for said angle of acquisition, a single 2D projection image formed by a representative parameter for each pixel; modifying the angle of acquisition by means of a relative rotation between the sheet of light and the sample, combined with a relative vertical translation between the sheet of light and the sample, and repeating the previous steps; and generating a 3D image of each of the samples from the set of 2D projection images that are produced.

Abstract: In certain embodiments, the invention relates to systems and methods for in vivo tomographic imaging of fluorescent probes and/or bioluminescent reporters, wherein a fluorescent probe and a bioluminescent reporter are spatially co-localized (e.g., located at distances equivalent to or smaller than the scattering mean free path of light) in a diffusive medium (e.g., biological tissue). Measurements obtained from bioluminescent and fluorescent modalities are combined per methods described herein.