Abstract: A device for vacuum-assisted in vivo delivery of an agent into cells of tissue includes a housing that defines a chamber and at least one opening into the chamber. A port extends through the housing, is remote from the at least one opening, and is connectable to a vacuum source. The port is configured to communicate vacuum pressure from the vacuum source to the chamber for drawing tissue through the opening and into the chamber.

Abstract: Techniques for treating subjects with an injected agent include applying vacuum pressure to tissue adjacent the injection and optionally electroporating said tissue. A device for applying such treatment includes a housing defining a receptacle and an opening into the receptacle. A port extends through the housing remote from the opening and is connectable to a vacuum source, such that the port can communicate vacuum pressure from the vacuum source to the receptacle. The device includes an insert that is receivable within the receptacle and that defines an internal chamber, which is in communication with the vacuum source when the insert resides in the receptacle. The insert includes at least one electrode positioned within the chamber and configured to deliver one or more electroporation pulses to a targeted portion of tissue extending through the at least one opening and at least momentarily held in the chamber responsive to the vacuum pressure.

Abstract: An injection device for in vivo delivery of an agent includes a tubular body defining a lumen that extends along a central axis that is oriented along a longitudinal direction. A distal end of the lumen is occluded and the tubular body defines at least one side-port extending from the lumen to an outer surface of the tubular body. The at least one side-port is elongated along the outer surface of the tubular body.

Abstract: A device for vacuum-assisted in vivo electroporation of tissue includes a housing that defines a chamber and at least one opening into the chamber. A port extends through the housing, is remote from the at least one opening, and is connectable to a vacuum source. The port is configured to communicate vacuum pressure from the vacuum source to the chamber. A plurality of electrodes are positioned within the chamber and are configured to deliver one or more electroporation pulses to a targeted portion of tissue extending through the opening and held in the chamber responsive to the vacuum pressure.