Abstract: A device for vacuum-assisted in vivo delivery of an agent into cells of tissue includes a housing that defines a chamber and at least one opening into the chamber. A port extends through the housing, is remote from the at least one opening, and is connectable to a vacuum source. The port is configured to communicate vacuum pressure from the vacuum source to the chamber for drawing tissue through the opening and into the chamber.

Abstract: Techniques for treating subjects with an injected agent include applying vacuum pressure to tissue adjacent the injection and optionally electroporating said tissue. A device for applying such treatment includes a housing defining a receptacle and an opening into the receptacle. A port extends through the housing remote from the opening and is connectable to a vacuum source, such that the port can communicate vacuum pressure from the vacuum source to the receptacle. The device includes an insert that is receivable within the receptacle and that defines an internal chamber, which is in communication with the vacuum source when the insert resides in the receptacle. The insert includes at least one electrode positioned within the chamber and configured to deliver one or more electroporation pulses to a targeted portion of tissue extending through the at least one opening and at least momentarily held in the chamber responsive to the vacuum pressure.

Abstract: The invention relates to compositions which are useful for inhibiting ribonucleotide reductase enzymes, including the mammalian ribonucleotide reductase enzyme. The compositions include, but are not limited to, linear peptides, cyclic peptides, peptide analogs, and peptidomimetics. Methods of using the compositions of the invention to treat cancer and viral and bacterial infections are disclosed.