Patents by Inventor Alison Tsan

Alison Tsan has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • COMPOSITIONS AND METHODS FOR DETECTION OF HUMAN PARAINFLUENZA VIRUSES 1-4 (HPIV 1-4)
    Publication number: 20240124946
    Abstract: Methods for the rapid detection of the presence or absence of Human Parainfluenza Viruses (HPIV), including HPIV 1-4 in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers and probes targeting HPIV 1-4, and kits are provided that are designed for the detection of target regions of HPIV 1-4. Also described are kits, reaction mixtures, and oligonucleotides (e.g., primer and probe) for the amplification and detection of HPIV 1-4.
    Type: Application
    Filed: February 4, 2022
    Publication date: April 18, 2024
    Inventors: Chitra Manohar, Ramani S. Ravirala, Jingtao Sun, Alison Tsan, Michelle E. Yee
  • DETECTION OF TARGET NUCLEIC ACID BY SOLID-PHASE MOLOGRAPHY
    Publication number: 20220298555
    Abstract: The present invention describes methods for performing real-time PCR for detection and quantitation of target nucleic acids using tagged oligonucleotide probes and solid-phase molography.
    Type: Application
    Filed: December 19, 2019
    Publication date: September 22, 2022
    Inventors: Dieter Heindl, Igor Kozlov, Nikolaus-Peter Stengele, Alison Tsan, Volker Gatterdam
  • Methods for performing multiplexed real-time PCR
    Patent number: 11345958
    Abstract: The present invention describes methods for performing higher multiplexed real-time PCR for detection and quantitation of target nucleic acids using tagged hydrolysis probes.
    Type: Grant
    Filed: May 7, 2021
    Date of Patent: May 31, 2022
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Igor Kozlov, Amar Gupta, Randall Saiki, Alison Tsan
  • Methods for performing multiplexed real-time PCR
    Patent number: 11198903
    Abstract: The present invention describes methods for performing higher multiplexed real-time PCR for detection and quantitation of target nucleic acids using tagged hydrolysis probes.
    Type: Grant
    Filed: October 7, 2019
    Date of Patent: December 14, 2021
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Igor Kozlov, Amar Gupta, Randall Saiki, Alison Tsan
  • METHODS FOR PERFORMING MULTIPLEXED REAL-TIME PCR
    Publication number: 20210269857
    Abstract: The present invention describes methods for performing higher multiplexed real-time PCR for detection and quantitation of target nucleic acids using tagged hydrolysis probes.
    Type: Application
    Filed: May 7, 2021
    Publication date: September 2, 2021
    Inventors: Igor Kozlov, Amar Gupta, Randall Saiki, Alison Tsan
  • Methods for performing multiplexed real-time PCR
    Patent number: 11034997
    Abstract: The present invention describes methods for performing higher multiplexed real-time PCR for detection and quantitation of target nucleic acids using tagged hydrolysis probes.
    Type: Grant
    Filed: September 15, 2017
    Date of Patent: June 15, 2021
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Igor Kozlov, Amar Gupta, Randall Saiki, Alison Tsan
  • Methods for performing multiplexed PCR
    Patent number: 11028433
    Abstract: The present invention describes methods for performing higher multiplexed PCR for detection and quantitation of target nucleic acids using tagged hydrolysis probes.
    Type: Grant
    Filed: September 15, 2017
    Date of Patent: June 8, 2021
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Igor Kozlov, Amar Gupta, Randall Saiki, Alison Tsan
  • Methods for performing multiplexed real-time PCR in a self-contained nucleic acid analysis pouch
    Patent number: 10590469
    Abstract: The present invention describes methods for performing higher multiplexed real-time PCR for detection and quantitation of target nucleic acids using TAGS hydrolysis probes.
    Type: Grant
    Filed: September 25, 2018
    Date of Patent: March 17, 2020
    Assignee: Roche Molecular Svstems, Inc.
    Inventors: Amar Gupta, Igor Kozlov, Randall Saiki, Alison Tsan
  • METHODS FOR PERFORMING MULTIPLEXED REAL-TIME PCR
    Publication number: 20200048682
    Abstract: The present invention describes methods for performing higher multiplexed real-time PCR for detection and quantitation of target nucleic acids using tagged hydrolysis probes.
    Type: Application
    Filed: October 7, 2019
    Publication date: February 13, 2020
    Inventors: Igor Kozlov, Amar Gupta, Randall Saiki, Alison Tsan
  • Methods and compositions for detecting mutation in the human EZH2 gene
    Patent number: 10260102
    Abstract: The invention comprises reagents and methods for detecting cancer-associated mutations in the human EZH2 gene. Further, a method of detecting the mutations and a method of treatment are disclosed.
    Type: Grant
    Filed: October 7, 2014
    Date of Patent: April 16, 2019
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Xiaoju Max Ma, Chitra Manohar, Alison Tsan
  • METHODS FOR PERFORMING MULTIPLEXED REAL-TIME PCR IN A SELF-CONTAINED NUCLEIC ACID ANALYSIS POUCH
    Publication number: 20190024155
    Abstract: The present invention describes methods for performing higher multiplexed real-time PCR for detection and quantitation of target nucleic acids using TAGS hydrolysis probes.
    Type: Application
    Filed: September 25, 2018
    Publication date: January 24, 2019
    Inventors: Amar Gupta, Igor Kozlov, Randall Saiki, Alison Tsan
  • METHODS FOR PERFORMING MULTIPLEXED PCR
    Publication number: 20180073056
    Abstract: The present invention describes methods for performing higher multiplexed PCR for detection and quantitation of target nucleic acids using tagged hydrolysis probes.
    Type: Application
    Filed: September 15, 2017
    Publication date: March 15, 2018
    Inventors: Igor Kozlov, Amar Gupta, Randall Saiki, Alison Tsan
  • METHODS FOR PERFORMING MULTIPLEXED REAL-TIME PCR
    Publication number: 20180073064
    Abstract: The present invention describes methods for performing higher multiplexed real-time PCR for detection and quantitation of target nucleic acids using tagged hydrolysis probes.
    Type: Application
    Filed: September 15, 2017
    Publication date: March 15, 2018
    Inventors: Igor Kozlov, Amar Gupta, Randall Saiki, Alison Tsan
  • Non-invasive early detection of solid organ transplant rejection by quantitative analysis of mixtures by deep sequencing of HLA gene amplicons using next generation systems
    Patent number: 9752189
    Abstract: The invention is a method of detecting or assessing solid organ graft (transplant) rejection by detecting donor-specific HLA alleles in a blood sample of a graft (transplant) recipient. The invention further comprises a method of detecting the presence of maternal cells in a blood sample of an offspring.
    Type: Grant
    Filed: May 8, 2014
    Date of Patent: September 5, 2017
    Assignees: Roche Molecular Systems, Inc., The Scripps Research Institute
    Inventors: Henry Erlich, Bryan Hoglund, Cherie Holcomb, Priscilla Moonsamy, Nick Newton, Melinda Rastrou, Nancy Schoenbrunner, Alison Tsan, Daniel Salomon
  • Complex mutations in the epidermal growth factor receptor kinase domain
    Patent number: 9738935
    Abstract: Six new mutations were found in exon 19 of the EGFR gene, the exon that is often mutated in tumors. The invention comprises methods of detecting the mutations, methods of prognosis and methods of predicting response to treatment based on the presence of absence of the mutations.
    Type: Grant
    Filed: October 30, 2012
    Date of Patent: August 22, 2017
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Wei-min Liu, Alison Tsan
  • COMPOSITIONS FOR IMPROVED ALLELE-SPECIFIC PCR
    Publication number: 20160369330
    Abstract: The present invention includes compositions for allele-specific amplification, utilizing an allele-specific oligonucleotide, at least partially complementary to more than one variant of the target sequence, but having at least one selective nucleotide complementary to only one variant of the target sequence and incorporating at least one G-damp nucleotide.
    Type: Application
    Filed: September 1, 2016
    Publication date: December 22, 2016
    Inventors: Veeraiah Bodepudi, Nancy Schoenbrunner, Alison Tsan
  • Allele-specific amplification
    Patent number: 9506111
    Abstract: The present invention includes a method of allele-specific amplification, utilizing an allele-specific oligonucleotide, at least partially complementary to more than one variant of the target sequence, but having a 3?-terminal nucleotide complementary to only one variant of the target sequence and having at least one nucleotide with a base covalently modified at the exocyclic amino group, wherein the allele-specific oligonucleotide is extended by a nucleotide-incorporating biocatalyst predominantly when hybridized to the variant of the target sequence for which it has said complementary 3?-terminal nucleotide.
    Type: Grant
    Filed: October 11, 2013
    Date of Patent: November 29, 2016
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Stephen G. Will, Alison Tsan, Nicolas Newton
  • Use of G-clamp for improved allele-specific PCR
    Patent number: 9476091
    Abstract: The present invention includes a method of allele-specific amplification, utilizing an allele-specific oligonucleotide, at least partially complementary to more than one variant of the target sequence, but having at least one selective nucleotide complementary to only one variant of the target sequence and incorporating at least one “G-clamp” nucleotide.
    Type: Grant
    Filed: September 21, 2012
    Date of Patent: October 25, 2016
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Veeraiah Bodepudi, Nancy Schoenbrunner, Alison Tsan
  • Primers with modified phosphate and base in allele-specific PCR
    Patent number: 9382581
    Abstract: The present invention includes a method of allele-specific amplification, utilizing an allele-specific oligonucleotide, at least partially complementary to more than one variant of the target sequence, but having at least one selective nucleotide complementary to only one variant of the target sequence and incorporating both a nucleotide with a base covalently modified at the exocyclic amino group and a modified phosphate.
    Type: Grant
    Filed: November 27, 2013
    Date of Patent: July 5, 2016
    Assignee: ROCHE MOLECULAR SYSTEMS, INC.
    Inventors: Stefanie Froehner, Dieter Heindl, Dirk Kessler, Nancy Schoenbrunner, Alison Tsan
  • METHODS AND COMPOSITIONS FOR DETECTING MUTATIONS IN THE HUMAN PI3KCA (PIK3CA) GENE
    Publication number: 20160160297
    Abstract: The invention comprises reagents and methods for detecting cancer-associated mutations in the human PI3KCA (PIK3CA) gene and assessing the patients based thereon.
    Type: Application
    Filed: February 5, 2016
    Publication date: June 9, 2016
    Inventor: Alison Tsan