Abstract: An integrated cell culture system may include one or more cell culture vessels, manipulation apparatus, pumping apparatus, cell release apparatus, monitoring apparatus, and a control apparatus. The control apparatus may be used to monitor and control the system to facilitate effective cell culturing. The cell release apparatus may be used to release a plurality of cells adhered to the cell culture surfaces of the cell culture vessels.

Abstract: The present invention relates generally to a multi-layer system for containing cells in culture, and a system for visualizing cells cultured in the multi-layered system. More specifically, the present invention relates to a multi-layer cell culture device having a specialized bottom plate and a microscope adaptor which can accommodate a microscope to allow microscopic visualization of cells cultured in the device.

Abstract: A spheroid cell culture article including: a frame having a chamber including: an opaque side wall surface; a top aperture; a gas-permeable, transparent bottom; and optionally a chamber annex surface and second bottom, and at least a portion of the transparent bottom includes at least one concave arcuate surface, is disclosed. Methods of making and using the article are also disclosed.

Abstract: A multilayered cell culture apparatus for the culturing of cells is disclosed. The cell culture apparatus is defined as an integral structure having a plurality of cell culture chambers in combination with tracheal space(s). The body of the apparatus has imparted therein gas permeable membranes in combination with tracheal spaces that will allow the free flow of gases between the cell culture chambers and the external environment. The flask body also includes an aperture that will allow access to the cell growth chambers by means of a needle or cannula. The size of the apparatus, and location of an optional neck and cap section, allows for its manipulation by standard automated assay equipment, further making the apparatus ideal for high throughput applications.

Abstract: A multilayered cell culture apparatus for the culturing of cells is disclosed. The cell culture apparatus is defined as an integral structure having a plurality of cell culture chambers in combination with tracheal space(s). The body of the apparatus has imparted therein gas permeable membranes in combination with tracheal spaces that will allow the free flow of gases between the cell culture chambers and the external environment. The flask body also includes an aperture that will allow access to the cell growth chambers by means of a needle or cannula. The size of the apparatus, and location of an optional neck and cap section, allows for its manipulation by standard automated assay equipment, further making the apparatus ideal for high throughput applications.

Abstract: A multilayered cell culture apparatus for the culturing of cells is disclosed. The cell culture apparatus is defined as an integral structure having a plurality of cell culture chambers in combination with tracheal space(s). The body of the apparatus has imparted therein gas permeable membranes in combination with tracheal spaces that will allow the free flow of gases between the cell culture chambers and the external environment. The flask body also includes an aperture that will allow access to the cell growth chambers by means of a needle or cannula. The size of the apparatus, and location of an optional neck and cap section, allows for its manipulation by standard automated assay equipment, further making the apparatus ideal for high throughput applications.

Abstract: A multilayered cell culture apparatus for the culturing of cells is disclosed. The cell culture apparatus is defined as an integral structure having a plurality of cell culture chambers in combination with tracheal space(s). The body of the apparatus has imparted therein gas permeable membranes in combination with tracheal spaces that will allow the free flow of gases between the cell culture chambers and the external environment. The flask body also includes an aperture that will allow access to the cell growth chambers by means of a needle or cannula. The size of the apparatus, and location of an optional neck and cap section, allows for its manipulation by standard automated assay equipment, further making the apparatus ideal for high throughput applications.

Abstract: A multilayered cell culture apparatus for the culturing of cells is disclosed. The cell culture apparatus is defined as an integral structure having a plurality of cell culture chambers in combination with tracheal space(s). The body of the apparatus has imparted therein gas permeable membranes in combination with tracheal spaces that will allow the free flow of gases between the cell culture chambers and the external environment. The flask body also includes an aperture that will allow access to the cell growth chambers by means of a needle or cannula. The size of the apparatus, and location of an optional neck and cap section, allows for its manipulation by standard automated assay equipment, further making the apparatus ideal for high throughput applications.

Abstract: An assembly for culturing cells includes a housing defining a reservoir for containing cell culture media and an opening in fluid communication with the reservoir. The cell culture assembly further includes a filter valve assembly configured to sealingly engage the opening. The filter valve assembly includes a flexible membrane, a contiguous side wall, an end wall, and a microbial filter. The end wall has an opening and extends across one end of the side wall to form a major cavity within the side wall and the end wall. The flexible membrane has a slit spanning the membrane and is disposable within the major cavity such that the membrane sealingly divides the major cavity into (i) a chamber formed between the end wall and the membrane and (ii) a minor cavity formed by the membrane and a portion of the side wall. The minor cavity is in fluid communication with the reservoir.

Abstract: The present invention relates generally to a multi-layer system for containing cells in culture, and a system for visualizing cells cultured in the multi-layered system. More specifically, the present invention relates to a multi-layer cell culture device having a specialized bottom plate and a microscope adaptor which can accommodate a microscope to allow microscopic visualization of cells cultured in the device.

Abstract: The present invention provides a method for treating cell culture vessels including multi-layer cell culture vessels with ultrasonic energy to dissociate cells growing on surfaces of cell culture vessels.

Abstract: The present invention provides a method for treating cell culture vessels including multi-layer cell culture vessels with ultrasonic energy to dissociate cells growing on surfaces of cell culture vessels.

Abstract: A cell culture vessel includes a housing defining a reservoir, an inlet and an outlet in fluid communication with the reservoir, a valve assembly, and an antimicrobial filter. The valve assembly includes (i) a side wall defining a passageway for fluid flow between the reservoir and the outlet, (ii) a floating element disposed in the passageway and configured to float on the culture medium, (iii) a stop feature configured to sealingly engage the floating element to prevent the culture medium from flowing from the reservoir through the outlet, and (iv) a capture feature configured to retain the floating element within the passageway and to allow fluid to flow from the reservoir through the passageway. The microbial filter is positioned such that air flowing in the outlet to the passageway passes through the filter.

Abstract: An assembly for culturing cells includes a housing defining a reservoir for containing cell culture media and an opening in fluid communication with the reservoir. The cell culture assembly further includes a filter valve assembly configured to sealingly engage the opening. The filter valve assembly includes a flexible membrane, a contiguous side wall, an end wall, and a microbial filter. The end wall has an opening and extends across one end of the side wall to form a major cavity within the side wall and the end wall. The flexible membrane has a slit spanning the membrane and is disposable within the major cavity such that the membrane sealingly divides the major cavity into (i) a chamber formed between the end wall and the membrane and (ii) a minor cavity formed by the membrane and a portion of the side wall. The minor cavity is in fluid communication with the reservoir.

Abstract: A multilayered cell culture apparatus for the culturing of cells is disclosed. The cell culture apparatus is defined as an integral structure having a plurality of flexible cell culture compartments in combination with a spacer material maintaining air space(s). The expandable compartments of the cell culture apparatus have imparted therein gas permeable membranes in combination with air spaces that will allow the free flow of gases between the cell culture compartments and the external environment. Furthermore an interconnecting passageway is provided between each compartment and between the cell culturing vessel and the external environment. The expandable vessel promotes the growth of large numbers of adherent or suspension cells by providing the volumes of nutrient medium and gaseous exchange.

Abstract: A class of designs is provided for a permeable support where the design principle includes a permeable support layer, such as a membrane, bonded midway between two arrays of wells or multiwell plates, attached at their bottom portions, forming a double-sided multiwell plate. Thus, opposite facing wells on either side of the permeable support layer are accessible by inversion of the double-sided multiwell plate. Well fluid is held in place in the novel multiwell plate by capillary forces in the case of aligned upper and lower well arrays or by surface tension on patterned well regions on a permeable membrane layer. No additional components are necessary to form compartments for fluid retention.