Abstract: A system is provided having a guide member with features, such as grid and axes, which spatially correlate to tissue with a lesion adjacent the guide member, and a template device upon the tissue aligned with the features having a first aperture over the guide member, and a second aperture over the tissue with the lesion. A microscope captures one or more images of the tissue via the second aperture at one or more locations each spatially correlated with a location selected or being mapped along the guide member in the first aperture. Prior to imaging and template device placement, marks are recorded along the guide member when part of a tracing sheet is positioned in a folded state over the lesion to establish spatial correlation of the features of the guide member to the lesion when in an unfolded state and positioned for use with the template device and microscope.