Patents by Inventor Allon Moshe Klein

Allon Moshe Klein has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20240043893
    Abstract: The present invention generally relates to microfluidics and labeled nucleic acids. For example, certain aspects are generally directed to systems and methods for labeling nucleic acids within microfluidic droplets or other compartments, for instance, arising from a cell. In one set of embodiments, particles may be prepared containing oligonucleotides that can be used to determine target nucleic acids, e.g., attached to the surface of the particles. The oligonucleotides may include “barcodes” or unique sequences that can be used to distinguish nucleic acids in a droplet from those in another droplet, for instance, even after the nucleic acids are pooled together or removed from the droplets. Certain embodiments of the invention are generally directed to systems and methods for attaching additional or arbitrary sequences to the nucleic acids within microfluidic droplets or other compartments, e.g.
    Type: Application
    Filed: July 14, 2023
    Publication date: February 8, 2024
    Applicant: President and Fellows of Harvard College
    Inventors: David A. Weitz, Huidan Zhang, John Heyman, Allon Moshe Klein
  • Patent number: 11746367
    Abstract: The present invention generally relates to microfluidics and labeled nucleic acids. In one aspect, the present invention is generally directed to a method, wherein the method includes providing a plurality of droplets comprising particles, the particles comprising oligonucleotides, and attaching a nucleic acid sequence to the oligonucleotides. Certain embodiments are generally directed to systems and methods for splitting a droplet into two or more droplets. Certain embodiments are generally directed to systems and methods for sorting fluidic droplets in a liquid.
    Type: Grant
    Filed: April 15, 2016
    Date of Patent: September 5, 2023
    Assignee: President and Fellows of Harvard College
    Inventors: David A. Weitz, Huidan Zhang, John Heyman, Allon Moshe Klein
  • Publication number: 20210379555
    Abstract: The present invention generally relates to microfluidics and labeled nucleic acids. For example, certain aspects are generally directed to systems and methods for labeling nucleic acids within microfluidic droplets. In one set of embodiments, the nucleic acids may include “barcodes” or unique sequences that can be used to distinguish nucleic acids in a droplet from those in another droplet, for instance, even after the nucleic acids are pooled together. In some cases, the unique sequences may be incorporated into individual droplets using particles and attached to nucleic acids contained within the droplets (for example, released from lysed cells). In some cases, the barcodes may be used to distinguish tens, hundreds, or even thousands of nucleic acids, e.g., arising from different cells or other sources.
    Type: Application
    Filed: May 18, 2021
    Publication date: December 9, 2021
    Applicants: President and Fellows of Harvard College, Vilnius University
    Inventors: David A. Weitz, Allon Moshe Klein, Ilke Akartuna, Linas Mazutis, Marc W. Kirschner
  • Patent number: 11052368
    Abstract: The present invention generally relates to microfluidics and labeled nucleic acids. For example, certain aspects are generally directed to systems and methods for labeling nucleic acids within microfluidic droplets. In one set of embodiments, the nucleic acids may include “barcodes” or unique sequences that can be used to distinguish nucleic acids in a droplet from those in another droplet, for instance, even after the nucleic acids are pooled together. In some cases, the unique sequences may be incorporated into individual droplets using particles and attached to nucleic acids contained within the droplets (for example, released from lysed cells). In some cases, the barcodes may be used to distinguish tens, hundreds, or even thousands of nucleic acids, e.g., arising from different cells or other sources.
    Type: Grant
    Filed: May 29, 2018
    Date of Patent: July 6, 2021
    Assignees: Vilnius University, President and Fellows of Harvard College
    Inventors: David A. Weitz, Allon Moshe Klein, Ilke Akartuna, Linas Mazutis, Marc W. Kirschner
  • Patent number: 10596541
    Abstract: The present invention generally relates to microfluidics and labeled nucleic acids. For example, certain aspects are generally directed to systems and methods for labeling nucleic acids within microfluidic droplets. In one set of embodiments, the nucleic acids may include “barcodes” or unique sequences that can be used to distinguish nucleic acids in a droplet from those in another droplet, for instance, even after the nucleic acids are pooled together. In some cases, the unique sequences may be incorporated into individual droplets using particles and attached to nucleic acids contained within the droplets (for example, released from lysed cells). In some cases, the barcodes may be used to distinguish tens, hundreds, or even thousands of nucleic acids, e.g., arising from different cells or other sources.
    Type: Grant
    Filed: October 3, 2017
    Date of Patent: March 24, 2020
    Assignees: President and Fellows of Harvard College, Vilnius University
    Inventors: David A. Weitz, Allon Moshe Klein, Ilke Akartuna, Linas Mazutis, Marc W. Kirschner
  • Publication number: 20180304222
    Abstract: The present invention generally relates to microfluidics and labeled nucleic acids. For example, certain aspects are generally directed to systems and methods for labeling nucleic acids within microfluidic droplets. In one set of embodiments, the nucleic acids may include “barcodes” or unique sequences that can be used to distinguish nucleic acids in a droplet from those in another droplet, for instance, even after the nucleic acids are pooled together. In some cases, the unique sequences may be incorporated into individual droplets using particles and attached to nucleic acids contained within the droplets (for example, released from lysed cells). In some cases, the barcodes may be used to distinguish tens, hundreds, or even thousands of nucleic acids, e.g., arising from different cells or other sources.
    Type: Application
    Filed: May 29, 2018
    Publication date: October 25, 2018
    Inventors: David A. Weitz, Allon Moshe Klein, Ilke Akartuna, Linas Mazutis, Marc W. Kirschner
  • Publication number: 20180087078
    Abstract: The present invention generally relates to microfluidics and labeled nucleic acids. In one aspect, the present invention is generally directed to a method, wherein the method includes providing a plurality of droplets comprising particles, the particles comprising oligonucleotides, and attaching a nucleic acid sequence to the oligonucleotides. Certain embodiments are generally directed to systems and methods for splitting a droplet into two or more droplets. Certain embodiments are generally directed to systems and methods for sorting fluidic droplets in a liquid.
    Type: Application
    Filed: April 15, 2016
    Publication date: March 29, 2018
    Applicant: President and Fellows of Harvard College
    Inventors: David A. Weitz, Huidan Zhang, Hohn Heyman, Allon Moshe Klein
  • Publication number: 20180071705
    Abstract: The present invention generally relates to microfluidics and labeled nucleic acids. For example, certain aspects are generally directed to systems and methods for labeling nucleic acids within microfluidic droplets. In one set of embodiments, the nucleic acids may include “barcodes” or unique sequences that can be used to distinguish nucleic acids in a droplet from those in another droplet, for instance, even after the nucleic acids are pooled together. In some cases, the unique sequences may be incorporated into individual droplets using particles and attached to nucleic acids contained within the droplets (for example, released from lysed cells). In some cases, the barcodes may be used to distinguish tens, hundreds, or even thousands of nucleic acids, e.g., arising from different cells or other sources.
    Type: Application
    Filed: October 3, 2017
    Publication date: March 15, 2018
    Inventors: David A. Weitz, Allon Moshe Klein, Ilke Akartuna, Linas Mazutis, Marc W. Kirschner
  • Publication number: 20150298091
    Abstract: The present invention generally relates to microfluidics and labeled nucleic acids. For example, certain aspects are generally directed to systems and methods for labeling nucleic acids within microfluidic droplets. In one set of embodiments, the nucleic acids may include “barcodes” or unique sequences that can be used to distinguish nucleic acids in a droplet from those in another droplet, for instance, even after the nucleic acids are pooled together. In some cases, the unique sequences may be incorporated into individual droplets using particles and attached to nucleic acids contained within the droplets (for example, released from lysed cells). In some cases, the barcodes may be used to distinguish tens, hundreds, or even thousands of nucleic acids, e.g., arising from different cells or other sources.
    Type: Application
    Filed: June 9, 2015
    Publication date: October 22, 2015
    Inventors: David A. Weitz, Allon Moshe Klein, Ilke Akartuna, Linas Mazutis, Marc W. Kirschner