Patents by Inventor Allon Moshe Klein
Allon Moshe Klein has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Publication number: 20240043893Abstract: The present invention generally relates to microfluidics and labeled nucleic acids. For example, certain aspects are generally directed to systems and methods for labeling nucleic acids within microfluidic droplets or other compartments, for instance, arising from a cell. In one set of embodiments, particles may be prepared containing oligonucleotides that can be used to determine target nucleic acids, e.g., attached to the surface of the particles. The oligonucleotides may include “barcodes” or unique sequences that can be used to distinguish nucleic acids in a droplet from those in another droplet, for instance, even after the nucleic acids are pooled together or removed from the droplets. Certain embodiments of the invention are generally directed to systems and methods for attaching additional or arbitrary sequences to the nucleic acids within microfluidic droplets or other compartments, e.g.Type: ApplicationFiled: July 14, 2023Publication date: February 8, 2024Applicant: President and Fellows of Harvard CollegeInventors: David A. Weitz, Huidan Zhang, John Heyman, Allon Moshe Klein
-
Patent number: 11746367Abstract: The present invention generally relates to microfluidics and labeled nucleic acids. In one aspect, the present invention is generally directed to a method, wherein the method includes providing a plurality of droplets comprising particles, the particles comprising oligonucleotides, and attaching a nucleic acid sequence to the oligonucleotides. Certain embodiments are generally directed to systems and methods for splitting a droplet into two or more droplets. Certain embodiments are generally directed to systems and methods for sorting fluidic droplets in a liquid.Type: GrantFiled: April 15, 2016Date of Patent: September 5, 2023Assignee: President and Fellows of Harvard CollegeInventors: David A. Weitz, Huidan Zhang, John Heyman, Allon Moshe Klein
-
Publication number: 20210379555Abstract: The present invention generally relates to microfluidics and labeled nucleic acids. For example, certain aspects are generally directed to systems and methods for labeling nucleic acids within microfluidic droplets. In one set of embodiments, the nucleic acids may include “barcodes” or unique sequences that can be used to distinguish nucleic acids in a droplet from those in another droplet, for instance, even after the nucleic acids are pooled together. In some cases, the unique sequences may be incorporated into individual droplets using particles and attached to nucleic acids contained within the droplets (for example, released from lysed cells). In some cases, the barcodes may be used to distinguish tens, hundreds, or even thousands of nucleic acids, e.g., arising from different cells or other sources.Type: ApplicationFiled: May 18, 2021Publication date: December 9, 2021Applicants: President and Fellows of Harvard College, Vilnius UniversityInventors: David A. Weitz, Allon Moshe Klein, Ilke Akartuna, Linas Mazutis, Marc W. Kirschner
-
Patent number: 11052368Abstract: The present invention generally relates to microfluidics and labeled nucleic acids. For example, certain aspects are generally directed to systems and methods for labeling nucleic acids within microfluidic droplets. In one set of embodiments, the nucleic acids may include “barcodes” or unique sequences that can be used to distinguish nucleic acids in a droplet from those in another droplet, for instance, even after the nucleic acids are pooled together. In some cases, the unique sequences may be incorporated into individual droplets using particles and attached to nucleic acids contained within the droplets (for example, released from lysed cells). In some cases, the barcodes may be used to distinguish tens, hundreds, or even thousands of nucleic acids, e.g., arising from different cells or other sources.Type: GrantFiled: May 29, 2018Date of Patent: July 6, 2021Assignees: Vilnius University, President and Fellows of Harvard CollegeInventors: David A. Weitz, Allon Moshe Klein, Ilke Akartuna, Linas Mazutis, Marc W. Kirschner
-
Patent number: 10596541Abstract: The present invention generally relates to microfluidics and labeled nucleic acids. For example, certain aspects are generally directed to systems and methods for labeling nucleic acids within microfluidic droplets. In one set of embodiments, the nucleic acids may include “barcodes” or unique sequences that can be used to distinguish nucleic acids in a droplet from those in another droplet, for instance, even after the nucleic acids are pooled together. In some cases, the unique sequences may be incorporated into individual droplets using particles and attached to nucleic acids contained within the droplets (for example, released from lysed cells). In some cases, the barcodes may be used to distinguish tens, hundreds, or even thousands of nucleic acids, e.g., arising from different cells or other sources.Type: GrantFiled: October 3, 2017Date of Patent: March 24, 2020Assignees: President and Fellows of Harvard College, Vilnius UniversityInventors: David A. Weitz, Allon Moshe Klein, Ilke Akartuna, Linas Mazutis, Marc W. Kirschner
-
Publication number: 20180304222Abstract: The present invention generally relates to microfluidics and labeled nucleic acids. For example, certain aspects are generally directed to systems and methods for labeling nucleic acids within microfluidic droplets. In one set of embodiments, the nucleic acids may include “barcodes” or unique sequences that can be used to distinguish nucleic acids in a droplet from those in another droplet, for instance, even after the nucleic acids are pooled together. In some cases, the unique sequences may be incorporated into individual droplets using particles and attached to nucleic acids contained within the droplets (for example, released from lysed cells). In some cases, the barcodes may be used to distinguish tens, hundreds, or even thousands of nucleic acids, e.g., arising from different cells or other sources.Type: ApplicationFiled: May 29, 2018Publication date: October 25, 2018Inventors: David A. Weitz, Allon Moshe Klein, Ilke Akartuna, Linas Mazutis, Marc W. Kirschner
-
Publication number: 20180087078Abstract: The present invention generally relates to microfluidics and labeled nucleic acids. In one aspect, the present invention is generally directed to a method, wherein the method includes providing a plurality of droplets comprising particles, the particles comprising oligonucleotides, and attaching a nucleic acid sequence to the oligonucleotides. Certain embodiments are generally directed to systems and methods for splitting a droplet into two or more droplets. Certain embodiments are generally directed to systems and methods for sorting fluidic droplets in a liquid.Type: ApplicationFiled: April 15, 2016Publication date: March 29, 2018Applicant: President and Fellows of Harvard CollegeInventors: David A. Weitz, Huidan Zhang, Hohn Heyman, Allon Moshe Klein
-
Publication number: 20180071705Abstract: The present invention generally relates to microfluidics and labeled nucleic acids. For example, certain aspects are generally directed to systems and methods for labeling nucleic acids within microfluidic droplets. In one set of embodiments, the nucleic acids may include “barcodes” or unique sequences that can be used to distinguish nucleic acids in a droplet from those in another droplet, for instance, even after the nucleic acids are pooled together. In some cases, the unique sequences may be incorporated into individual droplets using particles and attached to nucleic acids contained within the droplets (for example, released from lysed cells). In some cases, the barcodes may be used to distinguish tens, hundreds, or even thousands of nucleic acids, e.g., arising from different cells or other sources.Type: ApplicationFiled: October 3, 2017Publication date: March 15, 2018Inventors: David A. Weitz, Allon Moshe Klein, Ilke Akartuna, Linas Mazutis, Marc W. Kirschner
-
Publication number: 20150298091Abstract: The present invention generally relates to microfluidics and labeled nucleic acids. For example, certain aspects are generally directed to systems and methods for labeling nucleic acids within microfluidic droplets. In one set of embodiments, the nucleic acids may include “barcodes” or unique sequences that can be used to distinguish nucleic acids in a droplet from those in another droplet, for instance, even after the nucleic acids are pooled together. In some cases, the unique sequences may be incorporated into individual droplets using particles and attached to nucleic acids contained within the droplets (for example, released from lysed cells). In some cases, the barcodes may be used to distinguish tens, hundreds, or even thousands of nucleic acids, e.g., arising from different cells or other sources.Type: ApplicationFiled: June 9, 2015Publication date: October 22, 2015Inventors: David A. Weitz, Allon Moshe Klein, Ilke Akartuna, Linas Mazutis, Marc W. Kirschner