Abstract: The present invention relates to a method for continuous recovering of a protein from a fluid, comprising precipitating the protein in the fluid and separating the precipitated protein from the fluid. The invention also provides an inclined plate settler that can be used for such continuous protein recovering.

Abstract: The invention refers to a single-chain circular permuted caspase-2 comprising the following structure from N- to C-terminus: i) a small subunit of a caspase-2, or a functionally active variant thereof; and ii) a large subunit of a caspase-2, or a functionally active variant thereof, wherein said cp caspase-2 comprises one or more amino acid substitutions increasing P1? tolerance of said cp caspase-2 compared to a cp caspase-2 without said amino acid substitutions.

Abstract: A system and a method for on-demand reconstituting solid process material is disclosed. The system comprises a feeding device for continuously feeding said solid process material, a mixing vessel, optionally a hold tank, optionally one or more mixing reactors, and optionally a sterile filter unit, wherein the system is configured to operate continuously.

Abstract: Provided is a method for producing and/or purifying measles virus (MV) particles from a sample, the method comprising in sequential order the following steps loading a sample containing MV particles and one or more impurities onto a stationary phase material for carrying out flow-through chromatography to bind at least a fraction of the impurities contained in the sample and to produce a flow-through comprising at least a fraction of the MV particles contained in the sample; carrying out filtration, preferably ultrafiltration, and obtaining a retentate having an increased MV titer relative to the MV titer comprised in the flow-through. Further provided is a system for producing and/or purifying MV particles, comprising at least one bioreactor; a clarification unit, preferably a dead end filter unit, downstream to the bioreactor; a flow through chromatography unit downstream to the clarification unit; and a filtration unit, downstream to the flow through chromatography unit.

Abstract: This disclosure relates to a bottom section for being connected to an assembly for separating a solid component from a fluid. The assembly includes an inclined plate settler with at least one sedimentation channel for letting a solid component to be separated settle, said plate settler comprising a lower portion and an upper portion, wherein said at least one sedimentation channel extends from the lower portion to the upper portion. The bottom section is configured to be connected to the lower portion of the inclined plate settler. The bottom section comprises at least one inlet channel for feeding a fluid comprising the solid component to be separated to the plate settler, and at least one collection channel for collecting a settled solid component descending from the at least one sedimentation channel.

Abstract: The invention relates to a method and device which allows in real-time the determination of concentration, purity and potency of a biological product during purification and/or concentration processes in order to intervene into the process, either for process control or real time release. The properties of the process stream are continuously monitored by at least two online sensors and with the aid of multivariate statistical analysis so that concentration, purity and potency is determined in real time.

Abstract: The present invention provides novel methods of cell disruption and release of biomolecules from a cell. The invention comprises the use of positively and/or negatively charged microparticles comprising ground resin. It is particularly useful for purification of biomolecules from cell culture.

Abstract: The present invention provides a novel adsorbent composition for recovering biomolecules from a fluid. The composition comprises positively and negatively charged microparticles in the form of ground particles. The adsorbent is particularly useful for purification of biomolecules from the cell culture.

Abstract: This disclosure relates to a bottom section for being connected to an assembly for separating a solid component from a fluid. The assembly includes an inclined plate settler with at least one sedimentation channel for letting a solid component to be separated settle, said plate settler comprising a lower portion and an upper portion, wherein said at least one sedimentation channel extends from the lower portion to the upper portion. The bottom section is configured to be connected to the lower portion of the inclined plate settler. The bottom section comprises at least one inlet channel for feeding a fluid comprising the solid component to be separated to the plate settler, and at least one collection channel for collecting a settled solid component descending from the at least one sedimentation channel.

Abstract: This application relates to methods for purification of proteins such as antibodies using a hydrophilic polymer (e.g., PEG), a fatty acid (e.g., caprylic acid), and/or another agent (e.g., calcium chloride).

Abstract: This disclosure relates to methods for isolating antibodies from cell-free culture supernatant.

Abstract: The present invention provides a novel process for desalting protein solutions using micropore anion and cation exchange resins. In addition, a continuous process for desalting biopharmaceutical solutions was developed. Devices for use in the process for desalting biopharmaceutical solutions were also developed.

Abstract: The present invention provides novel methods of cell disruption and release of biomolecules from a cell. The invention comprises the use of positively and/or negatively charged microparticles comprising ground resin. It is particularly useful for purification of biomolecules from cell culture.

Abstract: The present invention relates a method for incubating liquids, to a method for preparing a biopharmaceutical drug, and to a device for the preparation of a biopharmaceutical drug.

Abstract: The present invention provides novel methods of cell disruption and release of biomolecules from a cell. The invention comprises the use of positively and/or negatively charged microparticles comprising ground resin. It is particularly useful for purification of biomolecules from cell culture.

Abstract: Disclosed is a method for refolding recombinantly produced polypeptides comprising the steps of (a)providing inclusion bodies comprising recombinantly produced polypeptides comprising the recombinantly produced polypeptides, (b)dissolving the inclusion bodies under chaotropic conditions in so as to obtain denatured recombinantly produced polypeptides, and (c)refolding the denatured recombinantly produced polypeptides inclusion bodies by addition of a refolding buffer,so as to obtain refolded re-combinantly produced polypeptides, and (d) further processing the refolded recombinantly produced polypeptides by at least one processing step, wherein at least steps (c) and (d) are performed in a tubular reactor in a continuous manner.

Abstract: This application relates to methods for purification of proteins such as antibodies using a hydrophilic polymer (e.g., PEG), a fatty acid (e.g., caprylic acid), and/or another agent (e.g., calcium chloride).

Abstract: The present invention provides novel methods of cell disruption and release of biomolecules from a cell. The invention comprises the use of positively and/or negatively charged microparticles comprising ground resin. It is particularly useful for purification of biomolecules from cell culture.

Abstract: The present invention provides a novel adsorbent composition for recovering biomolecules from a fluid. The composition comprises positively and negatively charged microparticles in the form of ground particles. The adsorbent is particularly useful for purification of biomolecules from the cell culture.

Abstract: The invention relates to an isolated waterproof polymeric nanomembrane comprising pores of different geometric shapes and of a controlled size between 10 and 1000 nm, which is larger than the thickness of the membrane, and a method of producing the same comprising the process steps a. Providing a sacrifice layer on a surface of a solid support; b. Providing a polymerized layer of less than 1000 nm thickness on the surface of the sacrifice layer, by depositing a mixture of a polymer or a polymer precursor with a geometrically undefined pore template which is larger than the thickness of the polymerized layer, optionally followed by polymerization and/or crosslinking; c. Removing the pore template to obtain the polymerized layer with a controlled pore size; and d. Removing the sacrifice layer, thereby separating the solid support from the polymerized layer.