Patents by Inventor Aloysius Wilhelmus Rudolphus Hubertus Teunissen
Aloysius Wilhelmus Rudolphus Hubertus Teunissen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11944104Abstract: The present invention relates to a variant polypeptide having maltogenic alpha-amylase activity wherein the polypeptide comprises an amino acid sequence, which, when aligned with an amino acid sequence of SEQ ID NO: 1, comprises an amino acid substitution F188L/I, S200N, and D261G, and optionally a further amino acid substitution T288P, wherein the amino acid substitutions are determined with reference to SEQ ID NO: 1, and wherein the polypeptide has an amino acid sequence which has at least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or at least 99% identity to the amino acid sequence of SEQ ID NO: 1. The invention also relates to a process for the preparation of a dough or a baked product wherein the variant polypeptide is used.Type: GrantFiled: April 4, 2019Date of Patent: April 2, 2024Assignee: DSM IP ASSETS B.V.Inventors: Joke Anneleen Putseys, Aloysius Wilhelmus Rudolphus Hubertus Teunissen, René Marcel De Jong, Jeroen Godefrooij, Helma Arina Stolze-Lagerweij
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Publication number: 20210161158Abstract: The present invention relates to a variant polypeptide having maltogenic alpha-amylase activity wherein the polypeptide comprises an amino acid sequence, which, when aligned with an amino acid sequence of SEQ ID NO: 1, comprises an amino acid substitution F188L/I, S200N, and D261G, and optionally a further amino acid substitution T288P, wherein the amino acid substitutions are determined with reference to SEQ ID NO: 1, and wherein the polypeptide has an amino acid sequence which has at least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or at least 99% identity to the amino acid sequence of SEQ ID NO: 1. The invention also relates to a process for the preparation of a dough or a baked product wherein the variant polypeptide is used.Type: ApplicationFiled: April 4, 2019Publication date: June 3, 2021Inventors: Joke Anneleen PUTSEYS, Aloysius Wilhelmus Rudolphus Hubertus TEUNISSEN, René Marcel DE JONG, Jeroen GODEFROOIJ, Helma Arina STOLZE-LAGERWEIJ
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Publication number: 20210115418Abstract: The present invention relates to a variant polypeptide having a lipase activity, wherein the polypeptide may be a polypeptide that has an amino acid sequence which has at least 70% identity to a mature amino acid sequence of SEQ ID NO: 1 or SEQ ID NO: 6, wherein the polypeptide comprises an amino acid substitution at position 295 and at least one further amino acid substitution at position 113, 121, 179 and/or 284, wherein the positions are defined with reference to SEQ ID NO: 1 or SEQ ID NO: 6. The invention further relates to a process for preparing a dough wherein a polypeptide as disclosed herein is used and baked product prepared from the dough.Type: ApplicationFiled: June 18, 2019Publication date: April 22, 2021Inventors: Joke Anneleen PUTSEYS, Neil CARR, René Marcel DE JONG, Aloysius Wilhelmus Rudolphus Hubertus TEUNISSEN
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Patent number: 10941421Abstract: The present invention relates to processes for producing ethanol from lignocellulosic hydrolysates comprising, hexoses, pentoses and acetic acid, whereby genetically modified yeast cells are use that comprise an exogenous gene encoding an acetaldehyde dehydrogenase and a bacterial gene encoding an enzyme with NAD+-linked glycerol dehydrogenase activity. The process is further characterised in that glycerol is present in or fed into the culture medium, whereby the modified yeast cell ferments the hexoses, pentoses, acetic acid and glycerol to ethanol. The invention further relates to yeast cells for use in such processes. The yeast cells advantageously comprise genetic modifications that improve glycerol utilization such as modifications that increase one or more of dihydroxyacetone kinase activity and transport of glycerol into the cell.Type: GrantFiled: May 3, 2018Date of Patent: March 9, 2021Assignee: DSM IP ASSETS B.V.Inventors: Johannes Adrianus Maria De Bont, Aloysius Wilhelmus Rudolphus Hubertus Teunissen, Paul Klaassen, Wouter Willem Antonius Hartman, Shimaira Van Beusekom
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Publication number: 20200140900Abstract: The invention relates to a cell capable of converting one or more pentose sugar and one or more hexose sugar into fermentation product constitutively expressing one or more heterologous or homologous polypeptide having the amino acid sequence set out in SEQ ID NO: 20, or a variant polypeptide thereof having at least 45% identity to SEQ ID NO 20. In an embodiment the heterologous polypeptide has glyoxalase activity.Type: ApplicationFiled: January 17, 2020Publication date: May 7, 2020Inventors: Maria Bernedina Elizabeth JONKERS, Paul KLAASSEN, Aloysius Wilhelmus Rudolphus Hubertus TEUNISSEN, Rene Marcel DE JONG
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Patent number: 10604773Abstract: The invention relates to a cell capable of converting one or more pentose sugar and one or more hexose sugar into fermentation product constitutively expressing one or more heterologous or homologous polypeptide having the amino acid sequence set out in SEQ ID NO: 20, or a variant polypeptide thereof having at least 45% identity to SEQ ID NO 20. In an embodiment the heterologous polypeptide has glyoxalase activity.Type: GrantFiled: March 13, 2019Date of Patent: March 31, 2020Assignee: DSM IP ASSETS B.V.Inventors: Maria Bernedina Elizabeth Jonkers, Paul Klaassen, Aloysius Wilhelmus Rudolphus Hubertus Teunissen, Rene Marcel De Jong
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Patent number: 10273507Abstract: The invention relates to a cell capable of converting one or more pentose sugar and one or more hexose sugar into fermentation product constitutively expressing one or more heterologous or homologous polypeptide having the amino acid sequence set out in SEQ ID NO: 20, or a variant polypeptide thereof having at least 45% identity to SEQ ID NO 20. In an embodiment the heterologous polypeptide has glyoxalase activity.Type: GrantFiled: October 15, 2013Date of Patent: April 30, 2019Assignee: DSM IP ASSETS B.V.Inventors: Maria Bernedina Elizabeth Jonkers, Paul Klaassen, Aloysius Wilhelmus Rudolphus Hubertus Teunissen, Rene Marcel De Jong
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Publication number: 20180371447Abstract: The present invention relates to eukaryotic cells which have the ability to isomerise xylose directly into xylulose. The cells have acquired this ability by transformation with nucleotide sequences coding for a xylose isomerase that has one or more specific sequence elements typical for isomerases having the ability of functional expression in yeasts, such as e.g. xylose isomerases obtainable from a bacterium of the genera Clostridium and Fusobacterium or a tunicate form the genus Ciona. The cell preferably is a yeast or a filamentous fungus, more preferably a yeast is capable of anaerobic alcoholic fermentation.Type: ApplicationFiled: September 4, 2018Publication date: December 27, 2018Inventors: Aloysius Wilhelmus Rudolphus Hubertus TEUNISSEN, Johannes Adrianus Maria De Bont
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Patent number: 10093914Abstract: The present invention relates to eukaryotic cells which have the ability to isomerize xylose directly into xylulose. The cells have acquired this ability by transformation with nucleotide sequences coding for a xylose isomerase that has one or more specific sequence elements typical for isomerases having the ability of functional expression in yeasts, such as e.g. xylose isomerases obtainable from a bacterium of the genera Clostridium and Fusobacterium or a tunicate form the genus Ciona. The cell preferably is a yeast or a filamentous fungus, more preferably a yeast is capable of anaerobic alcoholic fermentation.Type: GrantFiled: April 7, 2016Date of Patent: October 9, 2018Assignee: DSM IP ASSETS B.V.Inventors: Aloysius Wilhelmus Rudolphus Hubertus Teunissen, Johannes Adrianus Maria De Bont
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Publication number: 20180273929Abstract: The present invention relates to a polypeptide having asparaginase activity selected from the group consisting of: (i) a polypeptide having an amino acid sequence comprising the mature polypeptide sequence of SEQ ID NO: 1; (ii) a polypeptide comprising an amino acid sequence that has at least 50% sequence identity with the mature polypeptide sequence of SEQ ID NO: 1; (iii) a polypeptide encoded by a nucleic acid comprising a sequence that hybridizes under medium stringency conditions to the complementary strand of the mature polypeptide encoding sequence of SEQ ID NO: 2; and (iv) a polypeptide comprising an amino acid sequence encoded by a nucleic acid that has at least 50% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 2. The polypeptide may be used in the preparation of a food product.Type: ApplicationFiled: September 16, 2016Publication date: September 27, 2018Applicant: DSM IP ASSETS B.V.Inventors: Jan Metske VAN DER LAAN, Siew-loon OOI, Aloysius Wilhelmus Rudolphus Hubertus TEUNISSEN, Maarten Hotse WILBRINK
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Patent number: 9988649Abstract: The present invention relates to processes for producing ethanol from lignocellulosic hydrolysates comprising hexoses, pentoses and acetic acid, whereby genetically modified yeast cells are use that comprise an exogenous gene encoding an acetaldehyde dehydrogenase and a bacterial gene encoding an enzyme with NAD+-linked glycerol dehydrogenase activity. The process is further characterized in that glycerol is present in or fed into the culture medium, whereby the modified yeast cell ferments the hexoses, pentoses, acetic acid and glycerol to ethanol. The invention further relates to yeast cells for use in such processes. The yeast cells advantageously comprise genetic modifications that improve glycerol utilization such as modifications that increase one or more of dihydroxyacetone kinase activity and transport of glycerol into the cell.Type: GrantFiled: November 26, 2012Date of Patent: June 5, 2018Assignee: DSM IP ASSETS B.V.Inventors: Johannes Adrianus Maria De Bont, Aloysius Wilhelmus Rudolphus Hubertus Teunissen, Paul Klaassen, Wouter Willem Antonius Hartman, Shimaira Van Beusekom
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Publication number: 20160230162Abstract: The present invention relates to eukaryotic cells which have the ability to isomerise xylose directly into xylulose. The cells have acquired this ability by transformation with nucleotide sequences coding for a xylose isomerase that has one or more specific sequence elements typical for isomerases having the ability of functional expression in yeasts, such as e.g. xylose isomerases obtainable from a bacterium of the genera Clostridium and Fusobacterium or a tunicate form the genus Ciona. The cell preferably is a yeast or a filamentous fungus, more preferably a yeast is capable of anaerobic alcoholic fermentation.Type: ApplicationFiled: April 7, 2016Publication date: August 11, 2016Inventors: Aloysius Wilhelmus Rudolphus Hubertus Teunissen, Johannes Adrianus Maria De Bont
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Patent number: 9334488Abstract: The present invention relates to eukaryotic cells which have the ability to isomerize xylose directly into xylulose by transformation with nucleotide sequences encoding a xylose isomerase that has one or more specific sequence elements typical for isomerases which are functionally expressed in yeasts, such as xylose isomerases obtainable from bacteria of the genera Clostridium and Fusobacterium or a tunicate from the genus Ciona. The cell preferably is a yeast or a filamentous fungus capable of anaerobic alcoholic fermentation. The cells may further comprise one or more genetic modifications that increase the flux of the pentose phosphate pathway or, reduce unspecific aldose reductase activity. The cell preferably has the ability to produce a fermentation product such as ethanol, lactic acid, 3-hydroxy-propionic acid, ?-lactam antibiotics and cephalosporins. Also provided are processes for producing these fermentation products from xylose.Type: GrantFiled: December 24, 2009Date of Patent: May 10, 2016Assignee: DSM IP ASSETS B.V.Inventors: Aloysius Wilhelmus Rudolphus Hubertus Teunissen, Johannes Adrianus Maria De Bont
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Publication number: 20150275235Abstract: The invention relates to a cell capable of converting one or more pentose sugar and one or more hexose sugar into fermentation product constitutively expressing one or more heterologous or homologous polypeptide having the amino acid sequence set out in SEQ ID NO: 20, or a variant polypeptide thereof having at least 45% identity to SEQ ID NO 20. In an embodiment the heterologous polypeptide has glyoxalase activity.Type: ApplicationFiled: October 15, 2013Publication date: October 1, 2015Inventors: Maria Bernedina Elizabeth Jonkers, Paul Klaassen, Aloysius Wilhelmus Rudolphus Hubertus Teunissen, Rene Marcel De Jong
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Publication number: 20150176032Abstract: The present invention relates to processes for producing ethanol from lignocellulosic hydrolysates comprising hexoses, pentoses and acetic acid, whereby genetically modified yeast cells are use that comprise an exogenous gene encoding an acetaldehyde dehydrogenase and a bacterial gene encoding an enzyme with NAD+-linked glycerol dehydrogenase activity. The process is further characterised in that glycerol is present in or fed into the culture medium, whereby the modified yeast cell ferments the hexoses, pentoses, acetic acid and glycerol to ethanol. The invention further relates to yeast cells for use in such processes. The yeast cells advantageously comprise genetic modifications that improve glycerol utilization such as modifications that increase one or more of dihydroxyacetone kinase activity and transport of glycerol into the cell.Type: ApplicationFiled: November 26, 2012Publication date: June 25, 2015Inventors: Johannes Adrianus Maria De Bont, Aloysius Wilhelmus Rudolphus Hubertus Teunissen, Paul Klaassen, Wouter Willem Antonius Hartman, Shimaira Van Beusekom
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Publication number: 20110318790Abstract: The present invention relates to eukaryotic cells which have the ability to isomerise xylose directly into xylulose. The cells have acquired this ability by transformation with nucleotide sequences coding for a xylose isomerase that has one or more specific sequence elements typical for isomerases having the ability of functional expression in yeasts, such as e.g. xylose isomerases obtainable from a bacterium of the genera Clostridiumand Fusobacteriumor a tunicate form the genus Ciona. The cell preferably is a yeast or a filamentous fungus, more preferably a yeast is capable of anaerobic alcoholic fermentation.Type: ApplicationFiled: December 24, 2009Publication date: December 29, 2011Inventors: Aloysius Wilhelmus Rudolphus Hubertus Teunissen, Johannes Adrianus Maria De Bont